Evaluation of the use of RT-PCR for the early diagnosis of dengue fever

Grobusch, Martin P.; Niedrig, Matthias; Göbels, K; Klipstein‐Grobusch, Kerstin; Teichmann, Dieter

doi:10.1111/j.1469-0691.2006.01353.x

Cited by 22 publications

(10 citation statements)

References 11 publications

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“…A recent study documented ongoing CHIKV transmission in Kwale County, south of Mombasa [7]. Laboratory diagnosis of CHIKV and DENV during acute phase of illness can be done through virus isolation or real-time reverse-transcription polymerase chain reaction (RT-PCR) [11–13]. In a study by Riswari et al, CHIKV viremia preceded fever onset by up to six days, and CHIKV quantitative (qRT-PCR) testing on serial specimens indicated that viremia lasted for 11–13 days [14].…”

Section: Introductionmentioning

confidence: 99%

Dengue and Chikungunya Virus Infections among Young Febrile Adults Evaluated for Acute HIV-1 Infection in Coastal Kenya

et al. 2016

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BackgroundFever is common among patients seeking care in sub-Saharan Africa (sSA), but causes other than malaria are rarely diagnosed. We assessed dengue and chikungunya virus infections among young febrile adults evaluated for acute HIV infection (AHI) and malaria in coastal Kenya.MethodsWe tested plasma samples obtained in a cross-sectional study from febrile adult patients aged 18–35 years evaluated for AHI and malaria at urgent care seeking at seven health facilities in coastal Kenya in 2014–2015. Dengue virus (DENV) and chikungunya virus (CHIKV) were amplified using quantitative real-time reverse-transcription polymerase chain reaction. We conducted logistic regression analyses to determine independent predictors of dengue virus infection.Results489 samples that were negative for both AHI and malaria were tested, of which 43 (8.8%, 95% confidence interval [CI]: 6.4–11.7) were positive for DENV infection. No participant was positive for CHIKV infection. DENV infections were associated with clinic visits in the rainy season (adjusted odds ratio (AOR) = 3.0, 95% CI: 1.3–6.5) and evaluation at a private health facility (AOR 5.2, 95% CI: 2.0–13.1) or research health facility (AOR = 25.6, 95% CI: 8.9–73.2) instead of a public health facility.ConclusionA high prevalence of DENV infections was found in febrile young adult patients evaluated for AHI. Our data suggests that DENV, along with AHI and malaria, should be considered in the differential diagnosis of the adult patient seeking care for fever in coastal Kenya.

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Section: Introductionmentioning

confidence: 99%

Dengue and Chikungunya Virus Infections among Young Febrile Adults Evaluated for Acute HIV-1 Infection in Coastal Kenya

et al. 2016

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“…Case identification has become increasingly important in the differential diagnosis of the acute febrile illnesses and hemorrhagic fever of unknown flavivirus infections during the early symptomatic phase. Currently, molecular techniques (reverse transcription-PCR [RT-PCR]) have widely replaced traditional virus isolation methods in the early detection of DENV infections and differentiation of the four DENV serotypes and other related flavivirus infections (12,21). Although molecular detection provides a promising sensitivity rate and rapid diagnosis, the molecular approach is costly, as it requires specialized laboratory equipment and experienced technicians; these are notable limitations in many developing countries where dengue disease is endemic (29).…”

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confidence: 99%

Development of an Antigen Capture Immunoassay Based on Monoclonal Antibodies Specific for Dengue Virus Serotype 2 Nonstructural Protein 1 for Early and Rapid Identification of Dengue Virus Serotype 2 Infections

Qiu

Wen

et al. 2009

Clin Vaccine Immunol

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The dengue virus (DENV) has four distinct serotypes (DENV1, DENV2, DENV3, and DENV4) that require differentiation for effective prevention of morbid diseases. The recently developed DENV1-specific NS1 antigen capture enzyme-linked immunosorbent assay (ELISA) based on the monoclonal antibodies (MAbs) that recognize distinct epitopes on nonstructural protein 1 (NS1) of a specific DENV serotype is convenient and cost-effective, but assays have not yet been developed for DENV serotypes 2 to 4. This paper describes the development and validation of a DENV2-specific NS1 antigen capture ELISA by selection and optimization of the pair of well-characterized MAbs that recognized epitopes specific for DENV2 NS1 from a large panel of MAbs. The DENV2 NS1 ELISA displayed exclusive sensitivity with the DENV2 serotype and did not cross-react with the other three DENV serotypes. The sensitivity and specificity of the DENV2 NS1 ELISA were 83.3% (25/30) and 100% (504/504) when used to test 30 acute-phase serum samples from patients infected with DENV2 identified by virus isolation or reverse transcription-PCR serotyping and 504 serum samples from healthy individuals, respectively. The specificity of this assay was also evaluated using a panel of serum samples which were positive for DENV1, other flaviviruses, and nonflaviviruses; no cross-reactions were observed in these clinical samples. The DENV2 NS1 ELISA was eightfold more sensitive than a commercially available serotype-cross-reactive NS1 ELISA (Panbio Diagnostics, Brisbane, Australia) when the two assays were used to test the DENV2-infected cell culture supernatants in parallel. The Panbio NS1 ELISA displayed variation in sensitivity between DENV serotypes. The DENV2-specific NS1 antigen capture ELISA can be used as a tool for the rapid identification of DENV2 infections.The mosquito-borne dengue disease is caused by infections with the dengue virus (DENV). It is a major public health problem that affects over 100 tropical and subtropical countries. Moreover, the geographical territory affected by this disease has increased markedly since the 1970s; contributing factors may include a lack of control over the mosquito vector, global warming, viral evolution, and increased ease of global travel (23). The DENV has four known serotypes (DENV1, DENV2, DENV3, and DENV4).

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“…Also, there were no differences in the course of the disease in these patients. It is known that serum dengue IgM appears to peak about 2 weeks after the primary infection, declining to undetectable levels over 2-3 months whereas its persistence in CSF is about one month 20,21 .…”

Section: Discussionmentioning

confidence: 99%

“…PCR is usually positive in the first week of infection 20 . This test was negative in all of our cases probably due the longer time necessary to develop GBS after an infection; frequently more than one week.…”

Section: Discussionmentioning

confidence: 99%

Oligosymptomatic dengue infection: a potential cause of Guillain Barré syndrome

Soares

Cabral‐Castro

Oliveira³

et al. 2008

Arq. Neuro-Psiquiatr.

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-Background: Dengue infection may cause neurological manifestations such as encephalitis, myelitis, mononeuropathies, acute disseminated encephalomyelitis, and Guillain Barré syndrome (GBS). In endemic regions, the infection course can be oligosymptomatic making difficult the diagnosis of the neurological picture associated with dengue infection. Objective: To report dengue infection and GBS association, even in oligosymptomatic cases of this infection. Method: During the dengue epidemic in Rio de Janeiro city we looked for GBS cases, testing IgM antibodies for dengue and dengue polymerase chain reaction (PCR) in the cerebrospinal fluid (CSF) and serum. Results: We report seven cases (46.6%), presenting dengue positive IgM in serum but with poor or without clinical symptoms of the previous infection. Two of them had also positive IgM antibodies in CSF. Conclusion: These data show that search for dengue infection should be a routine in GBS cases living in endemic areas.Key WoRDS: dengue, polyradiculoneuritis, radiculoneuropathy, CSF, Guillain Barré syndrome. Dengue oligossintomática: causa potencial de síndrome de Guillain BarréResumo -Introdução: o vírus da dengue pode determinar várias manifestações neurológicas como: encefalite, mielite, mononeuropatias, encefalomielite disseminada aguda e a síndrome de Guillain Barré (SGB). em regiões endêmicas a infecção pode ser oligosintomática dificultando o diagnóstico neurológico associado a infecção por dengue. Objetivo: Relatar a associação entre SGB e o vírus da dengue, principalmente em formas oligosintomáticas da infecção. Método: Durante epidemia pelo vírus da dengue na cidade do Rio de Janeiro, casos de SGB foram selecionados e tiveram suas amostras de soro e líquido cefalorraqueano (LCR) testadas para anticorpos dengue IgM e PCR para dengue. Resultados: Descrevemos sete casos (46,6%) com diagnóstico de SGB, apresentando IgM positiva para dengue no soro, porém com poucos ou nenhum sintoma infeccioso prévio. Dois pacientes também apresentavam IgM positiva no LCR. Conclusão: estes dados sugerem que a pesquisa para o vírus da dengue deve ser realizada em todos os casos de SGB em áreas endêmicas.PALAvRAS-ChAve: dengue, polirradiculoneurite, radiculoneuropatia, líquido cefalorraqueano e síndrome de

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Evaluation of the use of RT-PCR for the early diagnosis of dengue fever

Cited by 22 publications

References 11 publications

Dengue and Chikungunya Virus Infections among Young Febrile Adults Evaluated for Acute HIV-1 Infection in Coastal Kenya

Dengue and Chikungunya Virus Infections among Young Febrile Adults Evaluated for Acute HIV-1 Infection in Coastal Kenya

Development of an Antigen Capture Immunoassay Based on Monoclonal Antibodies Specific for Dengue Virus Serotype 2 Nonstructural Protein 1 for Early and Rapid Identification of Dengue Virus Serotype 2 Infections

Oligosymptomatic dengue infection: a potential cause of Guillain Barré syndrome

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