Evaluation of Total Phenolic Contents and Antioxidant Activity of Carrot Callus Extracts as Affected by Phenylalanine Precursor

Abstract

Carrot (Daucus carota) is a valuable plant with both therapeutic and horticultural potential. Stem, petiole and root derived calli of carrot were obtained on solid MS supplemented with 1 mg/l BAP + 2 mg/l NAA. Callus cultures supplemented with different L-phenylalanine (PHE) concentrations under light and dark conditions were evaluated for their antioxidant activity and total phenolic contents. The authors showed that PHE supplementation in Daucus carota cultures was necessary to raise the extraction yield percentage. Antioxidant assays such as DPPH scavenging activity and β-carotene bleaching have been carried out. In DPPH system, callus extracts from different explants grown under light conditions displayed lower DPPH radical scavenging activity at all PHE levels compared with that grown under dark conditions. Moreover, under both light and dark conditions callus cultures grown on MS supplemented with 1 mg/l BAP + 2 mg/l NAA plus 1000 mg/l PHE were recorded to yield the maximum values as antioxidant activities. Regarding β-carotene bleaching assay, under light condition the callus extract of stem, root and petiole recorded an inhibition of linolic acid 47.9, 41.43 and 39%) which is lower compared with dark grown cultures, respectively (52.46, 72.71 and 73.26%). Effect of different concentrations of phenylalanine on the total phenolic content of carrot callus extract examined under light conditions varied from 0.33 to 2 mg/g DW and 0.51 to 3.69 mg/g DW under dark conditions as expressed *Authors for correspondence: <usamajp@yahoo.com>. 208Arafa et al.by gallic acid equivalent (GAE). Our results have demonstrated that Daucus carota can be further extended to exploit its possible application for the preservation of food products as well as a health supplement and neutraceutical.

Rutin accumulation in gardenia calli cultures as a response to phenyl alanine and salicylic acid

“…Our findings (Table 3) are in line with the results reported earlier by Arafa et al (2015) and Arafa and Aly (2018), who detected that the highest scavenging activity values in calli of stem, root, and petiole explants of Daucua carota L. carota was recorded with using phenylalanine as amino acid precursor and Bemani et al (2012) who increased more amounts of Taxol and antioxidant activity by increment of phenylalanine supply in the extract of suspension-cultured hazel (Corylus avellana L.) cells. Furthermore, our investigations are in partial harmony with those of Ghasemzadeh et al (2012) who reported that SA improved the production of some chemical structures such as fisetin and anthocyanin who possess more potency as antioxidant activity than the control in ginger varieties and Hadian-Deljou et al (2017) who attained more total phenolics and antioxidant activity by salicylic acid application using 2 mM concentration at the earlier stages of cold storage and using 1 mM concentration at the end of cold storage for red delicious apples.…”

Evaluation of Acute Toxicity and Antiinflammatory Activity of Callus Extracts of Pulicaria Incisa (Lam.) Dc.

“…Regarding the HPLC analysis, 3-Hydroxy-4-methoxycinnamic acid, was appeared to be the main phenolic acid in callus infusion, this result is consistent with the findings of Arafa et al (2015) and Azeez et al (2017). These authors have reported that it is the major compound of callus produced under in vitro conditions.…”

Influence of Different Precursors on Content of Polyphenols in Camellia sinensis In Vitro Callus Culture

“…According to the data obtained by us, in tea callus cultures grown on the main medium and on media with various precursors, it was PhA that caused rapid activation of their accumulation and the effect remained throughout the cultivation period ( Figure 2 ). The stimulating effect of this precursor on the PCs formation has been repeatedly noted for various plant objects [ 54 , 55 , 56 , 57 ].…”