Evaluation of Tryptic Podocin Peptide in Urine Sediment Using LC-MS-MRM Method as a Potential Biomarker of Glomerular Injury in Dogs with Clinical Signs of Renal and Cardiac Disorders

Szczepankiewicz, Barbara; Bąchor, Remigiusz; Pasławski, Robert; Siwińska, Natalia; Pasławska, Urszula; Konieczny, Andrzej; Szewczuk, Zbigniew

doi:10.3390/molecules24173088

Cited by 14 publications

(16 citation statements)

References 35 publications

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“…It is used to detect many proteins (including podocin) in human and animal medicine [ 23 , 24 ]. Another method, LC-MS-MRM, which was applied in this study, has been well described and recommended in humans and small animals to detect selected podocin tryptic peptides [ 6 , 16 , 21 , 22 ]. Very recently, the authors have tested the applicability of the LC-MS-MRM method to identify the podocin tryptic peptide in feline [ 21 ] and canine [ 22 ] urine samples collected from animals with diagnosed chronic kidney disease and with glomerular injury (cardiorenal syndrome due to myxomatous mitral valve disease).…”

Section: Introductionmentioning

confidence: 99%

Evaluation of podocin in urine in horses using qualitative and quantitative methods

et al. 2020

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No sensitive method for diagnosing early kidney dysfunction in horses has been identified so far. Many studies carried out in humans and small animals show that podocin can be useful to diagnose various kidney diseases, mainly affecting the glomeruli. The aim of this study was to perform a qualitative and quantitative analysis of podocin in urine samples obtained from healthy horses, horses with clinical kidney dysfunction and horses at risk of acute kidney injury. The study objectives aimed to assess: (1) whether the selected podocin tryptic peptide for LC-MS-MRM allows for podocin detection in horse; and (2) whether the species-specific ELISA test makes this detection possible as well;, (3) whether the chosen methods are sensitive enough to detect kidney dysfunction and glomerular injury, (4) whether the results of the tests applying both methods correspond with one another, (5) whether the results correlate with the hematological and biochemical data. The signals that may indicate the presence of trypsin fragments of podocin were found in three healthy horses, all the horses diagnosed with kidney dysfunction and half of the animals at risk for acute kidney injury. The concentration of podocin, diagnosed with the ELISA test was as follows: from 0.19 to 1.2 ng/ml in healthy animals, from 0.19 to 20.0 ng/ml in AKI horses, from 0.29 to 5.71 ng/ml in horses at risk for acute kidney injury. The results of both methods corresponded significantly. Podocin may be a potential biomarker of clinical kidney disease in horses and may be used in the detection of glomerular injury. However, its use is limited by the possibility of physiological podocyturia. LC-MS-MRM seems to be a more sensitive method to evaluate the presence of podocin than the ELISA test, whilst selected tryptic peptides of podocin appear to apply to horses. The ELISA test showed greater effectiveness in excluding the disease than in confirming it.

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Section: Introductionmentioning

confidence: 99%

Evaluation of podocin in urine in horses using qualitative and quantitative methods

et al. 2020

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“…In this group of dogs late parameters of kidney injury such as creatinine and urine in serum were in reference range. According to Szczepankiewicz et al 5 , 6 during MMVD occurs for the detachment of podocytes from the glomeruli and their entry into the urine. Podocytes are highly specialized cells of the glomerular capsule, crucial from the point of view of selective plasma filtration and primary urine production.…”

Section: Discussionmentioning

confidence: 99%

“…Determining the number of podocytes in the urine is of great diagnostic importance, as it can be regarded as a reflection of irreversible changes in the glomeruli. 5 , 6 …”

Section: Discussionmentioning

confidence: 99%

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Evaluation of the diagnostic value of the renal resistive index as a marker of the subclinical development of cardiorenal syndrome in MMVD dogs

Szczepankiewicz

Pasławska

Siwińska

et al. 2021

J Renin Angiotensin Aldosterone Syst

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Introduction: Myxomatous mitral valve disease (MMVD) in dogs inevitably causes renal dysfunction. These interactions are known as the cardiorenal syndrome (CRS). The main aims of the study were to evaluate whether renal resistive index (RRI) may be useful as a non-invasive marker in subclinical stage of kidney injury in dogs with MMVD and to compare RRI with SDMA and Cyst C. Methods: Forty-four dogs were divided into two groups: control—15 healthy dogs and the heart group—29 dogs with MMVD (ACVIM class Cc). Study protocol included: anamnesis, clinical examination, electrocardiography, echocardiography, chest radiography, abdominal ultrasonography with measurements of the renal resistive index (RRI), urine, and blood analysis. Results: The RRI in the heart group was significantly higher 0.725 ± 0.035 versus control group 0.665 ± 0.028 ( p < 0.00085). The RRI cut-off point in dogs with stable chronic heart failure (CHF) under 8 years is 0.775, in older 0.64. RRI was similar in MMVD dogs treated with ACE-I + furosemide and dogs treated ACE-I + torasemide + pimobendan + spironolactone. There was no correlation between RRI and SDMA or Cyst C. Conclusion: RRI is more sensitive than creatinine, SDMA and Cyst C to reveal kidney injury in MMVD dogs class Cc younger than 8 years.

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“…The retention time of 3.78 min corresponds to the studied compound. The presence of an additional signal at 3.32 min is associated with the presence of cis-trans isomerism of the proline residues(29)…”

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Early detection of active glomerular lesions in dogs and cats using podocin

Szczepankiewicz

Pasławska

Nowak

et al. 2019

Journal of Veterinary Research

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In veterinary medicine, sensitive and specific markers of the early stages of renal failure still remain to be established. Podocytes could be a promising diagnostic tool in veterinary nephrology, especially in the differentiation of active pathological disease and glomerulopathies. Podocin is one of the robust proteins exploitable in detection of podocyturia. This article presents podocyte detection in urine for diagnostic purposes in veterinary medicine using a variety of methods. We describe the advantages and disadvantages of the immunohistochemical technique currently used, and of scanning microscopy, chromatography, and immunostaining. The identification of podocin-positive cells is a promising diagnostic tool in the detection of the early stages of glomerular basement membrane damage. The detection of renal failure prior to the occurrence of azotaemia is of high clinical importance from the clinical and scientific points of view.

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Evaluation of Tryptic Podocin Peptide in Urine Sediment Using LC-MS-MRM Method as a Potential Biomarker of Glomerular Injury in Dogs with Clinical Signs of Renal and Cardiac Disorders

Cited by 14 publications

References 35 publications

Evaluation of podocin in urine in horses using qualitative and quantitative methods

Evaluation of podocin in urine in horses using qualitative and quantitative methods

Evaluation of the diagnostic value of the renal resistive index as a marker of the subclinical development of cardiorenal syndrome in MMVD dogs

Early detection of active glomerular lesions in dogs and cats using podocin

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