Evaluation of Tualang honey as a supplement to fetal bovine serum in cell culture

The Effect of Tualang Honey on Human Periodontal Ligament Fibroblast Proliferation and Alkaline Phosphatase Level

“…This is consistent with the findings of Kannan et al (2009) where the highest cell proliferation for human osteoblast cell lines was also seen at 0.0195% honey in DMEM supplemented with 10% FBS and 1% penicillin streptomycin, after 72 h of incubation. In another study, Abuharfeil et al (1999) found that low concentrations of honey (0.1 and 0.2%) stimulated lymphocyte proliferation and activated phagocytes.…”

“…It can be deduced that honey can promote periodontal wound healing by stimulating proliferation of HPDLFs. Kannan et al (2009) reported that cell proliferation decreased in a dose-dependent manner. This is similar to our finding that at high concentrations (5%), honey was seen to inhibit HPDLFs proliferation.…”

“…Though the exact biochemical mechanism remains unclear, studies have shown that honey has anti-inflammatory, anti-oxidant, apoptotic and immunomodulatory properties (Hassan et al 2010). In addition, honey accelerates wound healing by forming new tissues and also has antimicrobial properties (Kannan et al 2009). …”

“…Honey is a substance produced by bees using nectar and sweet deposits from plants (Kannan et al 2009). It is acidic in nature (pH4) and consists of 80% sugars (fructose, glucose, sucrose, and maltose), 17-20% water and 4% of other substances (pollen grains, enzymes, hydrogen peroxide, amino acids, organic acids, polyphenols, vitamins and minerals).…”

“…Therefore, this study aimed to investigate the effect of honey on human periodontal ligament fibroblasts (HPDLFs) proliferation and ALP level, as the established medicinal properties of honey may prove beneficial to the healing and regeneration of the periodontium. A 3-(4,5-Dimethyl-2-thiazolyl)-2,5-diphenyl-2H-tetrazolium bromide (MTT) assay was performed to assess the cell proliferation based on a modification of the method described by Kannan et al (2009). The HPDLFs were plated in a 96-well plate at a density of 5000 cells/ well in 200 μL medium each and were allowed to attach for 24 h before being treated with different concentrations of honey (0.02, 0.3, 1 and 5%).…”