Evaluation of virulence factors in environmental isolates of Vibrio species

Rodrigues, Dália P.; Ribeiro, Roseli V.; Alves, Ronaldo Mendes; Hofer, Ernesto

doi:10.1590/s0074-02761993000400016

Cited by 24 publications

(15 citation statements)

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“…Markedly, the DNase was detected only in three species, V. parahaemolyticus, V. alginolyticus, and V. diabolicus, which were more or less related in phylogenetic affinities. The extracellular DNase acts as endonuclease contributing to DNA hydrolysis, thus expediting the bacterial virulence (Rodrigues et al, ). Phospho‐lipase production was shown by all the species (73%) except V. aestuarianus, but the lipase was detected from only seven isolates.…”

Section: Resultsmentioning

confidence: 99%

“…Amylase activity was examined by adding Gram's iodine onto the colonies grown in TSA supplemented with 0.2% (wt/vol) soluble starch (Rodrigues, Ribeiro, Alves, & Hofer, 1993). Isolates were streaked on DNase test agar (MB Cell) and 1 N HCl was added to the medium with well-grown colonies to detect the DNase activity.…”

Section: Phenotypic Pathogenicity Testsmentioning

confidence: 99%

“…Extracellular enzymes were detected from most of the isolates. Due to the putative virulence triggered by the exoenzyme expression, these enzymes have been used as an indicator of health risks associated with vibrios isolated from clinical sources, environment, and foodstuff (Lafisca, Pereira, Giaccone, & Rodrigues, 2008;Rodrigues et al, 1993). Caseinolytic activity has been associated with high bacterial toxicity (Gunnlaugsdóttir & Gudmundsdóttir, 1997), and gelatinase enzymes were reported to hydrolyze collagen, hemoglobin and some other biologically active peptides (Vergis et al, 2002).…”

Section: Antimicrobial Susceptibility Testingmentioning

confidence: 99%

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Vibrio spp. from Yesso scallop (Patinopecten yessoensis) demonstrating virulence properties and antimicrobial resistance

Silva

Hossain

Dahanayake

et al. 2019

Journal of Food Safety

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We report the prevalence and characterization of Vibrio spp. isolated from marketed Yesso scallop (Patinopecten yessoensis) in Korea. A total of 30 isolates including, V. parahaemolyticus (n = 2), V. alginolyticus (n = 9), V. fluvialis (n = 7), V. diabolicus (n = 7), V. anguillarum (n = 4) and V. aestuarianus (n = 1) were isolated and identified. The phenotypic pathogenicity tests demonstrated that, 18 (60%), 21 (70%), 18 (60%), 7 (23%), 22 (73%), 21 (70%), 9 (30%), and 11 (33%) of the isolates were positive for DNase, protease, gelatinase, lipase, phospho‐lipase, amylase, slime production, and haemolysis, respectively. PCR assays revealed the prevalence of toxR, tlh, VAC, vfh, hupO, and VPI genes among the isolates with varying combinations. A close genetic affinity among V. alginolyticus and V. diabolicus strains was observed. Also the virulence genes specific to one Vibrio species were detected among other species as well. In addition, 29/30 (97%) isolates were multidrug resistant, while higher resistance rates were shown for ampicillin, colistin, vancomycin, and cephalothin. The results imply that the scallops in Korean markets harbor Vibrio spp., which are potentially virulent and multidrug resistant, thus their public health implications should not be underrated. Practical applications For many decades, vibrios are known for its importance in seafoodborne illnesses. Yesso scallop is the most popular and extensively cultured scallop variety in Korea. Therefore, we sought to assess the marketed fresh Yesso scallops for the prevalence and molecular characterization of Vibrio species. A total of 30 strains were isolated and identified by a series of biochemical tests, subsequent gyrB gene sequencing and phylogenetic analyses. Six Vibrio spp. were identified with V. alginolyticus as the most prevalent. Interestingly, V. alginolyticus was genetically similar to V. diabolicus. Besides, the virulence genes specific to V. alginolyticus and V. parahaemolyticus were observed in other species as well. It suggests that the detection of the species‐specific genes does not ensure the correct identification of pathogenic vibrios. Further, the occurrence of V. parahaemolyticus‐specific virulence genes in other Vibrio spp. potentially complicates the correct tracking of V. parahaemolyticus infections. In addition, 73% of these Vibrio spp. isolates showed multiple antibiotic resistance (MAR) indices higher than 0.2, which signifies their high risk of infection. Collectively, these results provide important evidence that not only the well‐known pathogenic vibrios like V. parahaemolyticus, but also other Vibrio spp. can act alike because of their similar characteristics.

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Section: Resultsmentioning

confidence: 99%

Section: Phenotypic Pathogenicity Testsmentioning

confidence: 99%

Section: Antimicrobial Susceptibility Testingmentioning

confidence: 99%

See 1 more Smart Citation

Vibrio spp. from Yesso scallop (Patinopecten yessoensis) demonstrating virulence properties and antimicrobial resistance

Silva

Hossain

Dahanayake

et al. 2019

Journal of Food Safety

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“…All bacterial isolates from the Nile tilapia culture water were subjected to the tests of virulence factor expression, with the help of the following exoenzymes: gelatinase (GEL), elastase (Elas), caseinase (CAS), lipase (LIP), phospholipase (Phos) and haemolytic activity (β-hemolysis in sheep blood) (β-hem), following the methodologies of Rust, Messing, and Iglewski (1994), Rodrigues, Ribeiro, Alves, and Hofer (1993) and Furniss and Donovan (1979).…”

Section: Detection Of Potential Virulence Factorsmentioning

confidence: 99%

<b>Aquatic microbiota diversity in the culture of Nile tilapia (<i>Oreochromis niloticus</i>) using bioflocs or periphyton: virulence factors and biofilm formation

Silva

Cavalcante

Carvalho

et al. 2016

Acta Sci. Anim. Sci.

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The following research isolated and identified the main bacterial groups present in the culture of juvenile Nile tilapia in the presence of bioflocs and/or periphyton. The strains were also tested for the production of exoenzymes, indicative of potential virulence factors, and ability to form biofilm. The water samples were taken from tilapia cultured in the presence of bioflocs (T1), in the presence of bioflocs and periphyton (T2), from traditional culture (T3) and from culture in the presence of periphyton (T4). In the growth and selection of the bacterial groups, pour plate method was used, along with the following media: Plate Count Agar (PCA - DIFCO), Aero Pseudo Selective Agar (GSP - Himedia) and Nutrient Agar (AN - Merck). 46 strains were isolated in the following distribution: T1 (n = 12); T2 (n = 10); T3 (n = 14) and T4 (n = 10). Among the isolates, the most frequent genera were: Pseudomonas spp., Aeromonas spp., Staphylococcus spp., Bacillus spp., Mycobacterium spp., Micrococcus spp., and Corybacterium spp. Bacterial isolates in treatments T1 and T3 tested positive for five virulence profiles each, while those isolated from T2 and T4 for two and three virulence profiles, respectively. Treatments in bioflocs and periphyton (T2) or only periphyton (T4) yielded bacteria of less pathogenic potentials. In relation to the fish growth, T1 and T4 resulted in a higher final weight.

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“…In the animal models, V. fluvialis and the cholera toxin (CT) produced by V. cholerae O1 strains confirmed skin permeability factor (SPF). However, the antibodies against CT did not neutralize the SPF of V. fluvialis (Rodrigues et al, 1993; Ahsan et al, 1988). …”

Section: Virulence Factorsmentioning

confidence: 92%

Vibrio fluvialis: an emerging human pathogen

et al. 2014

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Vibrio fluvialis is a pathogen commonly found in coastal environs. Considering recent increase in numbers of diarrheal outbreaks and sporadic extraintestinal cases, V. fluvialis has been considered as an emerging pathogen. Though this pathogen can be easily isolated by existing culture methods, its identification is still a challenging problem due to close phenotypic resemblance either with Vibrio cholerae or Aeromonas spp. However, using molecular tools, it is easy to identify V. fluvialis from clinical and different environmental samples. Many putative virulence factors have been reported, but its mechanisms of pathogenesis and survival fitness in the environment are yet to be explored. This chapter covers some of the major discoveries that have been made to understand the importance of V. fluvialis.

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Evaluation of virulence factors in environmental isolates of Vibrio species

Cited by 24 publications

References 0 publications

Vibrio spp. from Yesso scallop (Patinopecten yessoensis) demonstrating virulence properties and antimicrobial resistance

Vibrio spp. from Yesso scallop (Patinopecten yessoensis) demonstrating virulence properties and antimicrobial resistance

<b>Aquatic microbiota diversity in the culture of Nile tilapia (<i>Oreochromis niloticus</i>) using bioflocs or periphyton: virulence factors and biofilm formation

Vibrio fluvialis: an emerging human pathogen

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