Evaluation of wheat stillage for ethanol production by recombinant Zymomonas mobilis

Davis, Linda; Jeon, Yong Jin; Svenson, Charles J.; Rogers, Peter J.; Pearce, J. H.; Peiris, Paul

doi:10.1016/j.biombioe.2005.02.006

Cited by 72 publications

(31 citation statements)

References 23 publications

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“…Therefore, it is most suitable as an immobilization matrix for entrapping biomolecules and microorganisms (Bashay 2003). Traditionally, the yeast Saccharomyces cerevisiae has been used all over the world as the major ethanol fermenting microorganism (Davis et al 2005). However, in recent years, research is being focused on processes involving the gram-negative anaerobic bacterium, Zymomonas mobilis, because of its better fermentation attributes, such as high specific rates of sugar uptake as well as that it converts sugar almost stoichiometrically to ethanol and CO 2 , grows more rapidly and demonstrates highest ethanol productivity during fermenta-Ethanol fermentation of mahula flowers 417 tion when compared to other fermentation organisms (Queresi & Manderson 1995;Rogers et al 1997;Davis et al 2005Davis et al , 2006.…”

Section: Introductionmentioning

confidence: 99%

Ethanol fermentation of mahula (Madhuca latifolia) flowers using free and immobilized bacteria Zymomonas mobilis MTCC 92

2010

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Mahula (Madhuca latifolia L.) is a deciduous tree commonly found in the tropical rain forests of Asian and Australian continent. Corolla, the edible part of its flowers, is rich in fermentable sugar (37 ± 0.23%; on dry weight basis). Batch fermentation of mahula flowers was carried out using Zymomonas mobilis MTCC 92 free cells and cells immobilized in calcium alginate matrix. The ethanol productions were 122.9 ± 0.972 and 134.6 ± 0.104 g/kg flowers on dry weight basis using free and immobilized cells, respectively, after 96 h of fermentation, which showed that cells entrapped in calcium alginate matrix yielded 8.7% more ethanol than free cells. Further, the immobilized cells were physiologically active up to three more cycles of fermentation producing 132.7 ± 0.095, 130.5 ± 0.09 and 128.7 ± 0.056 g ethanol per kg flower in first, second and third cycle, respectively.

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Section: Introductionmentioning

confidence: 99%

Ethanol fermentation of mahula (Madhuca latifolia) flowers using free and immobilized bacteria Zymomonas mobilis MTCC 92

2010

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“…Both microorganisms performed quite similarly, producing ethanol concentrations of 29 and 23 g/L and obtaining yields of 0.47 and 0.40 g/product/g sugar consumed, respectively. Davis et al [17] have also used hemicellulosic hydrolyzate from wheat kernels, reaching an ethanol concentration of 11 g/L and a residual xylose of 12 g/L using the ZM4 (pZB5) strain without supplementation. In subsequent studies, 5 g/L yeast extract and 40 g/L glucose was added to the medium, obtaining 28 g/L ethanol and 2.6 g/L residual xylose after 18 h.…”

Section: Application Of Genetic Engineering In Z Mobilis Cellsmentioning

confidence: 99%

“…Some researchers also found it difficult in the pentose fermentation, generating residual concentrations xylose, as described by Davis et al [17], reaching 11 g/ L of bioethanol and 12 g/ L of residual xylose concentration during 11 hours of process. Therefore, as indicated by several authors and proven in this study, the metabolic adaptation processes, as well as optimizations using the recombinant strain of Z. mobilis must be continuous with respect to maximizing the production of ethanol and microbial biomass.…”

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METABOLIC ADAPTATION STRATEGY USING Zymomonas mobilis CP4 CELLS FOR THE PRODUCTION OF SECOND GENERATION ETHANOL

Martins

Borges

Reis³

et al. 2015

JBT

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During the past few decades, considerable effort has been made to utilize agricultural and forest residues as biomass feedstock for the production of bioethanol as an alternative fuel. The bacterium Zymomonas mobilis was shown to be extremely attractive for the production of second-generation ethanol from glucose of the cellulose fraction due to its ability to uptake high amounts of this sugar, resulting in high ethanol productivity values. However, the wild-type strains are unable to metabolize xylose that arises from the hemicellulose fraction. Molecular biology techniques were incorporated to render the strain used in this study capable of fermenting xylose into ethanol and thus increase the efficiency of secondgeneration ethanol production. Thus, the aim of this study was to evaluate the performance of a recombinant strain of Z. mobilis in simultaneous saccharification and co-fermentation (SSCF) processes, in which the fermentation of both sugars (glucose and xylose) occurs in one step. Regarding the genetic transformation,the 1,565 kb Z. mobilis plasmid pZMO1 was chemically synthesized and cloned into a synthetic vector that contains the E. coli and Z. mobilis replication checkmark origin,the XI, XK, TAL, and TKL genes and tetracycline resistance. Metabolic adaptation was performed by transferring the recombinant strain to media containing increased xylose concentrations. Then, an experimental response surface methodology was used to evaluatethe addition of glucose and xylose with different concentrations, as well as the incorporation of hemicellulosic hydrolyzate in different proportions. The recombinant Z. mobilis CP4 strain reached 25 g/L ethanol, confirming that approximately 50% of this pentose was consumed in the SSCF process when using 30% solids, 20.5% hemicellulose hydrolysate, 10 mg/L tetracycline, an enzyme load of 25 FPU/g cellulignin, and 10% of the initial inoculum.

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“…Outra alternativa para a produção de etanol a partir de pentoses é o emprego de micro-organismos recombinantes, os quais vêm sendo muito estudados (ELIASSON et al, 2000;DAVIS et al, 2005 ROBERTO et al, 1991;HAHN-HAGERDAL et al, 1994;NIGAM, 2001b;2002;CHANDEL et al, 2007;FERREIRA;DUSSÁN; CADETE et al, 2012;LIN et al, 2012;SILVA et al, 2012;BELLIDO et al, 2013;HICKERT et al, 2013 (NIGAM et al, 1985), insetos habitantes de madeira (TOIVOLA et al, 1984;SUH et al, 2003), madeira em decomposição (TOIVOLA et al, 1984; CADETE et al, 2009) SLININGER et al, 1987). A via das fosfopentoses consiste de uma fase oxidativa que converte hexoses fosfato em pentoses fosfato, suprindo o NADPH necessário na via biossintética, e uma fase não-oxidativa, na qual as pentoses fosfato são convertidas em hexoses fosfato e trioses fosfato (JEFFRIES, 1983).…”

Section: Micro-organismos Produtores De Etanolunclassified

Produção de bioetanol a partir de hidrolisado hemicelulósico de bagaço de cana-de-açúcar empregando as leveduras Scheffersomyces (Pichia) stipitis NRRL Y-7124 e Candida shehatae UFMG HM 52.2 visando à aplicação em bioprocessos com campo eletromagnético

Dussán¹

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Aos técnicos do laboratório por sempre estarem presentes para me auxiliar no meu trabalho.Às queridas e atenciosas Sandra, Cida e Bia pelo apoio e auxílio.A minhas queridas amigas Raquel, Ellen, Débora e em especial a Adriana por toda a compreensão, paciência, apoio e carinho. Porque graças a elas tenho minha segunda família no Brasil.Marly, Deize, Andreia, Lilian, Beto, Aron, Alec e meu quase irmãozinho Felipe, em fim a todos os Dilon, que me acolheram com muito carinho em sua família.Aos colegas e amigos com os quais compartilhei meus dias de trabalho: Eduardo Machado, Bruna Karadi, Samira, Rafael, Sabrina, Danielle, Juliana, Mauricio, Otavio, Paulo, Bruna Caroline, Ivy, Patricia Miléo, Luciana Chaud, Priscila Vaz, Bruno Guedes, Rafael Cunha, Flavio, Juan Daniel, Brunosideo e a minha amiga equatoriana Cris.A meus novos amigos da UENF: Geraldo, Patrícia Rodrigues, Diana Catalina, Wanessa e Renê Lemos.Ao Departamento de Biotecnologia -DEBIQ -EEL da Universidade de São Paulo -USP, pela oportunidade da realização do Doutorado. Atualmente é grande o interesse pelo aproveitamento da biomassa vegetal visando à obtenção de bioetanol de segunda geração. Neste contexto, o presente trabalho teve como objetivo avaliar a produção de etanol de segunda geração a partir de hidrolisado hemicelulósico de bagaço de cana-de-açúcar pelas leveduras Scheffersomyces stipitis NRRL Y-7124 e Candida shehatae UFMG HM 52.2 visando à aplicação em bioprocessos com campo eletromagnético. Salienta-se ainda que a importância do presente trabalho se dá, não apenas pelo caráter inovador desta pesquisa, mas também pelo fato do bioeletromagnetismo e particularmente biorreatores assistidos por campos eletromagnéticos constituírem uma recente e promissora área de pesquisa na biotecnologia onde é possível estudar alternativas que resultem no aumento do rendimento e/ou da produtividade do processo de fermentação. Numa primeira etapa, foram realizados experimentos em bateladas simples em meio contendo hidrolisado hemicelulósico de bagaço de cana-de-açúcar previamente destoxificado e suplementado com extrato de levedura para a avaliação da influência das variáveis pH inicial , agitação e vazão de ar no fator de conversão de açúcares em etanol para cada uma das leveduras, de acordo com um planejamento fatorial completo 2 3 com três repetições no ponto central. As melhores condições operacionais obtidas para a levedura S. stipitis NRRL Y-7124 foram 100 rpm, 0,7 vvm e pH inicial de 6,50 com um k L a inicial de 3 h -1 as quais exerceram uma influência positiva sobre o processo fermentativo, obtendo-se valores de Y P/S de 0,16 g/g e Q P de 0,10 g L -1 h -1. Para a levedura C. shehatae UFMG HM 52.2 as condições foram 100 rpm, 0,1 vvm e pH inicial de 6,50 com um k L a inicial de 0,2 h -1 obtendo-se valores de Y P/S de 0,44 g/g e Q P de 0,25 g L -1 h -1. Segundo os resultados obtidos, observou-se que a levedura C. shehatae apresentou maiores valores de fator de rendimento e produtividade em etanol quando comparados à levedura S. stipitis. Posteriormente, foi av...

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Evaluation of wheat stillage for ethanol production by recombinant Zymomonas mobilis

Cited by 72 publications

References 23 publications

Ethanol fermentation of mahula (Madhuca latifolia) flowers using free and immobilized bacteria Zymomonas mobilis MTCC 92

Ethanol fermentation of mahula (Madhuca latifolia) flowers using free and immobilized bacteria Zymomonas mobilis MTCC 92

METABOLIC ADAPTATION STRATEGY USING Zymomonas mobilis CP4 CELLS FOR THE PRODUCTION OF SECOND GENERATION ETHANOL

Produção de bioetanol a partir de hidrolisado hemicelulósico de bagaço de cana-de-açúcar empregando as leveduras Scheffersomyces (Pichia) stipitis NRRL Y-7124 e Candida shehatae UFMG HM 52.2 visando à aplicação em bioprocessos com campo eletromagnético

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