Evaluations of Different Sources of Saccharomyces cerevisiae to Binding Capacity of Aflatoxin B1 Utilizing their Adsorption Isotherms

Gonçalves, Bruna Caroline Marques; Gonçalves, Cristiana; Rosim, Roice Eliana; Oliveira, Carlos Augusto Fernandes de; Corassin, Carlos Humberto

doi:10.17756/jfcn.2017-048

Cited by 7 publications

(4 citation statements)

References 24 publications

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“…The adsorption values of AFB 1 observed under our study were similar to those stated by Pereyra et al [32] for S. cerevisiae cell walls. Higher efficiences of ZEN, OTA and AFB 1 adsorption at lower concentration of yeast cell walls or β -glucan preparations and higher concentrations of toxins in test tubes were presented by other authors [21,40,45,46,47].…”

Section: Discussionmentioning

confidence: 54%

Candida utilis ATCC 9950 Cell Walls and β(1,3)/(1,6)-Glucan Preparations Produced Using Agro-Waste as a Mycotoxins Trap

Bzducha-Wróbel

Bryła

Gientka

et al. 2019

Toxins

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Mycotoxins are harmful contaminants of food and feed worldwide. Feed additives with the abilities to trap mycotoxins are considered substances which regulate toxin transfer from feed to tissue, reducing its absorption in animal digestive tract. Market analysis emphasizes growing interest of feed producers in mycotoxins binders obtained from yeast biomass. The aim of the study was to prescreen cell walls (CW) and β(1,3)/(1,6)-glucan (β-G) preparations isolated from Candida utilis ATCC 9950 cultivated on waste potato juice water with glycerol as adsorbents for aflatoxin B1 (AFB1), zearalenone (ZEN), ochratoxin A (OTA), deoxynivalenol (DON), nivalenol (NIV), T-2 toxin (T-2) and fumonisin B1 (FB1). The adsorption was studied in single concentration tests at pH 3.0 and 6.0 in the presence of 1% of the adsorbent and 500 ng/mL of individual toxin. Evaluated CW and β-G preparations had the potential to bind ZEN, OTA and AFB1 rather than DON, NIV, T-2 toxin and FB1. The highest percentage of adsorption (about 83%), adsorption capacity (approx. 41 µg/ g preparation) and distribution coefficient (458.7mL/g) was found for zearalenone when CW preparation was used under acidic conditions. Higher protein content in CW and smaller particles sizes of the formulation could influence more efficient binding of ZEN, OTA, DON and T-2 toxin at appropriate pH compared to purified β-G. Obtained results show the possibility to transform the waste potato juice water into valuable Candida utilis ATCC 9950 preparation with mycotoxins adsorption properties. Further research is important to improve the binding capacity of studied preparations by increasing the active surface of adsorption.

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Section: Discussionmentioning

confidence: 54%

Candida utilis ATCC 9950 Cell Walls and β(1,3)/(1,6)-Glucan Preparations Produced Using Agro-Waste as a Mycotoxins Trap

Bzducha-Wróbel

Bryła

Gientka

et al. 2019

Toxins

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“…According to their findings, the mean AFM1 concentration in raw milk samples was 10.7 ± 0.89 ppb, indicating that the raw milk samples exceeded the EU permissible limits (50 ng•L −1 ) and Egyptian standards (50 ng•L −1 ) of AFM1 in milk; the rate of AFM1 detoxification was between 86.1% and 97.7%. In a study, highly active sodium bentonite (SB) soil (SB-E) was used to absorb AF, the results of which showed the maximum The application of enzymes and microorganisms in AF bio-detoxification is a good alternative to conventional techniques in the food industry [189][190][191][192][193][194][195][196][197][198][199][200][201][202][203][204][205] (Figure 5a,b). There are two mechanisms for AF detoxification by microbial methods, these are: cell wall component adhesion and microbial enzymes.…”

Section: Methods Of Aflatoxin Detoxificationmentioning

confidence: 99%

Characteristics, Occurrence, Detection and Detoxification of Aflatoxins in Foods and Feeds

Nazhand

Durazzo²,

Lucarini³

et al. 2020

Foods

112

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Mycotoxin contamination continues to be a food safety concern globally, with the most toxic being aflatoxins. On-farm aflatoxins, during food transit or storage, directly or indirectly result in the contamination of foods, which affects the liver, immune system and reproduction after infiltration into human beings and animals. There are numerous reports on aflatoxins focusing on achieving appropriate methods for quantification, precise detection and control in order to ensure consumer safety. In 2012, the International Agency for Research on Cancer (IARC) classified aflatoxins B1, B2, G1, G2, M1 and M2 as group 1 carcinogenic substances, which are a global human health concern. Consequently, this review article addresses aflatoxin chemical properties and biosynthetic processes; aflatoxin contamination in foods and feeds; health effects in human beings and animals due to aflatoxin exposure, as well as aflatoxin detection and detoxification methods.

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“…The carbohydrate component of the yeast cell wall is a common site for toxin binding, although the toxin may have a different binding site. The decrease in toxin by β-glucan can be caused by the formation of a toxin-glucan molecular complex on the van der Waals bond through adsorption isotherm because this mechanism will not change the molecular structure of β-glucan; however, there will be a change in energy due to the interaction [27,28,30,80].…”

Section: Aflatoxin-reducing Activity Of Native Yeast and β-Glucanmentioning

confidence: 99%

“…Yeast has shown an ability to reduce aflatoxins through an isotherm adsorption mechanism [27]. β-glucan from yeast cell walls has potential as a mycotoxin binder, with an adsorption mechanism involving different adsorption centers and can easily access hydrogen bonds and ionic or hydrophobic interactions [28].…”

Section: Introductionmentioning

confidence: 99%

Antifungal and Aflatoxin-Reducing Activity of β-Glucan Isolated from Pichia norvegensis Grown on Tofu Wastewater

Utama

Suraloka

Rialita

et al. 2021

Foods

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Yeast can be isolated from tofu wastewater and the cell wall in the form of β-glucan can act as a natural decontaminant agent. This study aimed to isolate and characterize native yeast from tofu wastewater, which can be extracted to obtain β-glucan and then identify the yeast and its β-glucan activity regarding antifungal ability against Aspergillus flavus and aflatoxin-reducing activity towards aflatoxin B1 (AFB1) and B2 (AFB2). Tofu wastewater native yeast was molecularly identified, and the growth observed based on optical density for 96 h and the pH also measured. β-glucan was extracted from native yeast cell walls with the acid-base method and then the inhibition activity towards A. flavus was tested using the well diffusion method and microscopic observation. AFB1 and AFB2 reduction were identified using HPLC LC-MS/MS. The results showed that the native yeast isolated was Pichia norvegensis with a β-glucan yield of 6.59%. Pichia norvegensis and its β-glucan showed an inhibition zone against Aspergillus flavus of 11.33 ± 4.93 and 7.33 ± 3.51 mm, respectively. Total aflatoxin-reducing activity was also shown by Pichia norvegensis of 26.85 ± 2.87%, and β-glucan of 27.30 ± 1.49%, while AFB1- and AFB2-reducing activity by Pichia norvegensis was 36.97 ± 3.07% and 27.13 ± 1.69%, and β-glucan was 27.13 ± 1.69% and 32.59 ± 4.20%, respectively.

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Evaluations of Different Sources of Saccharomyces cerevisiae to Binding Capacity of Aflatoxin B1 Utilizing their Adsorption Isotherms

Cited by 7 publications

References 24 publications

Candida utilis ATCC 9950 Cell Walls and β(1,3)/(1,6)-Glucan Preparations Produced Using Agro-Waste as a Mycotoxins Trap

Candida utilis ATCC 9950 Cell Walls and β(1,3)/(1,6)-Glucan Preparations Produced Using Agro-Waste as a Mycotoxins Trap

Characteristics, Occurrence, Detection and Detoxification of Aflatoxins in Foods and Feeds

Antifungal and Aflatoxin-Reducing Activity of β-Glucan Isolated from Pichia norvegensis Grown on Tofu Wastewater

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