Evanescent wave DNA-aptamer biosensor based on long period gratings for the specific recognition of E. coli outer membrane proteins

A metrological assessment of grating-based optical fiber sensors is proposed with the aim of providing an objective evaluation of the performance of this sensor category. Attention was focused on the most common parameters, used to describe the performance of both optical refractometers and biosensors, which encompassed sensitivity, with a distinction between volume or bulk sensitivity and surface sensitivity, resolution, response time, limit of detection, specificity (or selectivity), reusability (or regenerability) and some other parameters of generic interest, such as measurement uncertainty, accuracy, precision, stability, drift, repeatability and reproducibility. Clearly, the concepts discussed here can also be applied to any resonance-based sensor, thus providing the basis for an easier and direct performance comparison of a great number of sensors published in the literature up to now. In addition, common mistakes present in the literature made for the evaluation of sensor performance are highlighted, and lastly a uniform performance assessment is discussed and provided. Finally, some design strategies will be proposed to develop a grating-based optical fiber sensing scheme with improved performance.

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“…Later, Queirós et al [83] reported an LPG functionalized with aptamers for the detection of E. Coli outer proteins with a LOD of 1 nM.…”

Section: Biosensorsmentioning

confidence: 99%

Towards a Uniform Metrological Assessment of Grating-Based Optical Fiber Sensors: From Refractometers to Biosensors

et al. 2017

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“…Not only antibodies but also nucleic acids, such as oligonucleotidic probes (DNA) [8,52], peptide nucleic acid probes (PNA) [53][54][55], and aptamers [34,35,56,57], have been also coupled to OFGs for the detection of different targets, consisting of short oligonucleotides, genomic DNA, and even proteins or small molecules (Table 2). Finally, a series of other BREs, based on biointeractions such as enzymatic processes [58,59], biotin/streptavidin affinity [18,33,[60][61][62], virus-specific recognition (bacteriophage T4/Escherichia coli bacteria) [63][64][65][66] and biomimetic affinity processes with molecularly imprinted polymers (MIPs) [67], have been implemented (Table 3).…”

Section: Applicationsmentioning

confidence: 99%

Biosensing with optical fiber gratings

et al. 2017

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Optical fiber gratings (OFGs), especially longperiod gratings (LPGs) and etched or tilted fiber Bragg gratings (FBGs), are playing an increasing role in the chemical and biochemical sensing based on the measurement of a surface refractive index (RI) change through a label-free configuration. In these devices, the electric field evanescent wave at the fiber/surrounding medium interface changes its optical properties (i.e. intensity and wavelength) as a result of the RI variation due to the interaction between a biological recognition layer deposited over the fiber and the analyte under investigation. The use of OFG-based technology platforms takes the advantages of optical fiber peculiarities, which are hardly offered by the other sensing systems, such as compactness, lightness, high compatibility with optoelectronic devices (both sources and detectors), and multiplexing and remote measurement capability as the signal is spectrally modulated. During the last decade, the growing request in practical applications pushed the technology behind the OFG-based sensors over its limits by means of the deposition of thin film overlays, nanocoatings, and nanostructures, in general. Here, we review efforts toward utilizing these nanomaterials as coatings for high-performance and low-detection limit devices. Moreover, we review the recent development in OFG-based biosensing and identify some of the key challenges for practical applications. While high-performance metrics are starting to be achieved experimentally, there are still open questions pertaining to an effective and reliable detection of small molecules, possibly up to single molecule, sensing in vivo and multi-target detection using OFG-based technology platforms.

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“…Beyond imaging, optical fibres have also been used extensively for sensing purposes. Optical fibre sensors have been explored for a wide range of applications including sensing of temperature [31][32][33], pressure [33,34], enzyme activity [35,36], presence of nucleic acids [36,37], pH [38,39], and oxygen saturation [38]. Detailed reviews can be found elsewhere [33,34,36,[40][41][42].…”

Section: Optical Fibres: From Telecommunications To Biomedical Imaginmentioning

confidence: 99%

Demonstrating the Use of Optical Fibres in Biomedical Sensing: A Collaborative Approach for Engagement and Education

Ehrlich

Parker

McNicholl

et al. 2020

Sensors

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This paper demonstrates how research at the intersection of physics, engineering, biology and medicine can be presented in an interactive and educational way to a non-scientific audience. Interdisciplinary research with a focus on prevalent diseases provides a relatable context that can be used to engage with the public. Respiratory diseases are significant contributors to avoidable morbidity and mortality and have a growing social and economic impact. With the aim of improving lung disease understanding, new techniques in fibre-based optical endomicroscopy have been recently developed. Here, we present a novel engagement activity that resembles a bench-to-bedside pathway. The activity comprises an inexpensive educational tool (<$70) adapted from a clinical optical endomicroscopy system and tutorials that cover state-of-the-art research. The activity was co-created by high school science teachers and researchers in a collaborative way that can be implemented into any engagement development process.

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Evanescent wave DNA-aptamer biosensor based on long period gratings for the specific recognition of E. coli outer membrane proteins

Cited by 49 publications

References 22 publications

Towards a Uniform Metrological Assessment of Grating-Based Optical Fiber Sensors: From Refractometers to Biosensors

Towards a Uniform Metrological Assessment of Grating-Based Optical Fiber Sensors: From Refractometers to Biosensors

Biosensing with optical fiber gratings

Demonstrating the Use of Optical Fibres in Biomedical Sensing: A Collaborative Approach for Engagement and Education

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