Even juvenile leaves of tobacco exhibit programmed cell death

Horii, Manabu; Marubashi, Wataru

doi:10.5511/plantbiotechnology.22.339

Cited by 7 publications

(9 citation statements)

References 21 publications

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“…In some species, such as tobacco (Nicotiana tabacum), almost 100% of leaf protoplasts yielded cell colonies and ultimately plants (Bourgin et al, 1979), suggesting that dividing protoplasts can be obtained from various differentiated cells. To attain high regeneration rates, it was recommended that young and nonstressed tissues be used for protoplast isolation (Chupeau et al, 1974) and that plant growth conditions be adjusted to avoid premature cell death (Horii and Marubashi, 2005).…”

Section: Introductionmentioning

confidence: 99%

Characterization of the Early Events Leading to Totipotency in an Arabidopsis Protoplast Liquid Culture by Temporal Transcript Profiling

Chupeau

Granier

Pichon³

et al. 2013

The Plant Cell

101

109

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The molecular mechanisms underlying plant cell totipotency are largely unknown. Here, we present a protocol for the efficient regeneration of plants from Arabidopsis thaliana protoplasts. The specific liquid medium used in our study leads to a high rate of reentry into the cell cycle of most cell types, providing a powerful system to study dedifferentiation/regeneration processes in independent somatic cells. To identify the early events in the establishment of totipotency, we monitored the genome-wide transcript profiles of plantlets and protoplast-derived cells (PdCs) during the first week of culture. Plant cells rapidly dedifferentiated. Then, we observed the reinitiation and reorientation of protein synthesis, accompanied by the reinitiation of cell division and de novo cell wall synthesis. Marked changes in the expression of chromatin-associated genes, especially of those in the histone variant family, were observed during protoplast culture. Surprisingly, the epigenetic status of PdCs and well-established cell cultures differed, with PdCs exhibiting rare reactivated transposons and epigenetic changes. The differentially expressed genes identified in this study are interesting candidates for investigating the molecular mechanisms underlying plant cell plasticity and totipotency. One of these genes, the plant-specific transcription factor ABERRANT LATERAL ROOT FORMATION4, is required for the initiation of protoplast division.

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Section: Introductionmentioning

confidence: 99%

Characterization of the Early Events Leading to Totipotency in an Arabidopsis Protoplast Liquid Culture by Temporal Transcript Profiling

Chupeau

Granier

Pichon³

et al. 2013

The Plant Cell

101

109

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“…Horii and Marubashi (2005) showed evidence of PCD (DNA laddering by agarose gel electrophoresis and DNA fragmentation by terminal deoxynucleotidyl transferasemediated dUTP nick-end labeling assays) for juvenile and young leaves of Nicotiana tabacum. However, other reported that PCD-associated cleavage is restricted exclusively to the final brown stage of leaf development in cucumber .…”

Section: Introductionmentioning

confidence: 99%

“…Lee and Chen (2002) found no indication of DNA laddering in senescing rice leaves. Whereas, in all of these studies, the whole leaves from the seedlings have been used (Leśniewska et al 2000, Horii and Marubashi 2005, Lee and Chen 2002.…”

Section: Introductionmentioning

confidence: 99%

Detection of DNA ladder during cotyledon senescence in cotton

Qingen¹,

Liu²,

Yu³

et al. 2008

Biologia plant.

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The asynchronous programmed cell death (PCD) in different regions of the cotton cotyledon was studied during senescence. We showed that changes in chlorophyll contents do not occur at the same time in different parts of the cotyledon. By using light microscopy (LM) and the terminal deoxynucleotidyl transferase (TdT)-mediated dUTP nick end in situ labelling (TUNEL) methods, the symptoms of cell death were detected in cotyledon margins earlier than in the central part. DNA ladders were detected by gel electrophoresis in senescent cotyledon margins and in the centre respectively, but not in the whole cotyledons.

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“…However, Faraco and coworkers (2011) reported that the pluripotent protoplast does not necessarily exhibit plant regeneration because it primarily depends on the source of protoplasts. In somatic hybridization, the development of protoplast is a key step, and it can be obtained from a variety of differentiated young and non-stressed tissues/cells from, for example, leaf mesophyll and roots that can divide and produce cell colonies and then grow into plants (Horii and Marubashi, 2005;Eeckhaut and Van-Huylenbroeck, 2011). Protoplasts have several advantages in investigating plant cell lines, differentiation, dedifferentiation, stem-ness, and pluripotency (Grafi, 2004).…”

Section: Introductionmentioning

confidence: 99%

Protoplast isolation and plant regeneration in two cultivated coriander varieties, Co-1 and RS

Ali¹,

Mujib²,

Zafar³

et al. 2018

bta

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An efficient and reproducible protocol for plant regeneration from protoplasts has been developed in two Coriandrum sativum varieties-Co-1 and Rajendraswathi (RS). The RS coriander is rich in oil and shows resistance to diseases whereas Co-1 is known for better yield. In the present study, protoplast isolation-the first step of fusion was undertaken in cotyledon-derived embryogenic cell suspensions by using enzymatic solutions of different concentrations. Digestion with 2% cellulase Onozuka R-10, 1% pectinase and 0.2% macerozyme R-10 showed the maximum yield of protoplasts (4.60 × 10 6 /g of callus) when mannitol was added as the osmoticum. The cell division and plating efficiency, however, varied with the different levels of plant growth regulators (PGRs) used. In a liquid medium amended with 0.2 mg/l α-naphthalene acetic acid (NAA) and 0.2 mg/l 6-benzyladenine (BA), protoplasts showed a higher plating efficiency (6.2% in Co-1 and 5.4% in RS) compared to other PGR treatments. On a 2,4-dichlorophenoxy acetic acid (2,4-D)-supplemented medium, cells divided fast, produced micro-colonies, and formed the callus on which a heterogeneous mixture of somatic embryos was produced in both the varieties tested. The maximum number of somatic embryos was produced (47/callus mass) in a medium to which 1 mg/l 2,4-D was added. On a BA and gibberellic acid (GA 3)-supplemented medium,somatic embryos germinated into plantlets, and the maximum germination frequency (81%)was noted in a medium to which1 mg/l BA and 0.5 mg/l GA 3 was added. A successful somatic embryo-mediated plant regeneration was achieved from protoplasts in both coriander varieties, and the regenerated plants were morphologically similar to mother plants. The whole process of "protoplast to plant recovery", enzymatic mixtures, osmotic condition, and role of PGRs are discussed in this article.

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Even juvenile leaves of tobacco exhibit programmed cell death

Cited by 7 publications

References 21 publications

Characterization of the Early Events Leading to Totipotency in an Arabidopsis Protoplast Liquid Culture by Temporal Transcript Profiling

Characterization of the Early Events Leading to Totipotency in an Arabidopsis Protoplast Liquid Culture by Temporal Transcript Profiling

Detection of DNA ladder during cotyledon senescence in cotton

Protoplast isolation and plant regeneration in two cultivated coriander varieties, Co-1 and RS

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