Evening and morning alterations in Obstructive Sleep Apnea red blood cell proteome

Abstract

This article presents proteomics data referenced in [1] Using proteomics-based evaluation of red blood cells (RBCs), we have identified differentially abundant proteins associated with Obstructive Sleep Apnea Syndrome (OSA). RBCs were collected from peripheral blood of patients with moderate/severe OSA or snoring at pre- (evening) and post-night (morning) polysomnography, so that proteome variations between these time points could be assessed. RBC cytoplasmic fraction depleted of hemoglobin, using Hemovoid™ system, were analyzed by two-dimensional fluorescence difference gel electrophoresis (2D-DIGE), the 2D image software-based analyzed and relevant differentially abundant proteins identified by mass spectrometry (MS). MS identified 31 protein spots differentially abundant corresponding to 21 unique proteins possibly due to the existence of post-translational modification regulations. Functional analysis by bioinformatics tools indicated that most proteins are associated with catalytic, oxidoreductase, peroxidase, hydrolase, ATPase and anti-oxidant activity. At morning a larger numbers of differential proteins including response to chemical stimulus, oxidation reduction, regulation of catalytic activity and response to stress were observed in OSA. The data might support further research in OSA biomarker discovery and validation.

Evening and morning peroxiredoxin-2 redox/oligomeric state changes in obstructive sleep apnea red blood cells: Correlation with polysomnographic and metabolic parameters

“…76 of these exhibited significant differences in abundance (fold change ≥ 1.2; Anova p < 0.05) between OSA and Snorer's at evening or morning times (Fig. 1 - Data in Brief [16]).…”

“…From these spots, 31 were identified by MS, corresponding to 21 unique proteins suggesting the existence of post-translational modification (PTM) regulations (Table 1-Data in Brief [16]).…”

“…2-Data in Brief [16]). Functional analysis by DAVID Bioinformatics Resources [18] showed that most identified proteins are associated with catalytic, oxidoreductase, peroxidase, hydrolase, ATPase and antioxidant activity and nucleoside binding.…”

“…Morning compared with evening in OSA showed a larger numbers of differential proteins thus a larger number of associated biological processes, which included response to chemical stimulus, oxidation reduction, regulation of catalytic activity and response to stress (Fig. 2-Data in Brief [16]).…”

“…RBC sample preparation, analysis by 2D-DIGE approach followed by mass spectrometry for protein identification and protein annotation and classification using dedicated bioinformatics tools are described in detail in [16]. …”