Evidence for hexasomic inheritance in <i>Chrysanthemum morifolium</i> Ramat. based on analysis of EST-SSR markers

Fan, Min; Gao, Yike; Wu, Zhicheng; Haider, Saba; Zhang, Qixiang

doi:10.1139/gen-2020-0155

Cited by 5 publications

(1 citation statement)

References 34 publications

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“…The application of transcriptome sequencing technology has resulted in a rapid increase in the number of EST and EST-SSR markers in many plants, including tiger lily ( Sun et al, 2022 ), common buckwheat ( Liu et al, 2022 ), vegetable soybean ( Zhang et al, 2013 ), and Chrysanthemum morifolium Ramat ( Fan et al, 2022 ). However, there have been relatively few reports describing EST-SSR molecular markers in A. esculentus .…”

Section: Discussionmentioning

confidence: 99%

Development and Application of Fruit Color-Related Expressed Sequence Tag-Simple Sequence Repeat Markers in Abelmoschus esculentus on the Basis of Transcriptome Sequencing

Luo

Liu

et al. 2022

Front. Plant Sci.

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Abelmoschus esculentus is a medicinal and edible plant that contains large amounts of active ingredients, including anthocyanins, polysaccharides, flavonoids, and terpenoids. However, because of a relative lack of molecular research, there are few molecular markers applicable for this plant species. In this study, on the basis of A. esculentus fruit color-related transcriptome sequencing data, we analyzed the patterns of simple sequence repeats (SSRs) in differentially expressed genes (DEGs) and revealed the biological processes and metabolic pathways associated with the related genes. We also designed primers for SSR loci to develop SSR molecular markers. Primers were synthesized using a DEG associated with a protein–protein interaction network. Polymorphic SSR markers were screened for the subsequent examination of A. esculentus germplasm resources and fruit color association analysis. The results indicated that 24.98% of the unigenes contained SSR motifs. Single-base (mononucleotide) repeats were the main SSRs, followed by trinucleotide and dinucleotide repeats. We selected 47 expressed sequence tag (EST)-SSR primer pairs for the genotyping of 153 A. esculentus varieties/lines. We ultimately obtained 21 EST-SSR markers suitable for genotyping. A generalized linear model-based association analysis detected two EST-SSR markers significantly associated with A. esculentus fruit color. In conclusion, several EST-SSR and SSR molecular markers in A. esculentus were developed in this study. The fruit color-associated markers may be useful for the molecular marker-assisted breeding of new A. esculentus varieties.

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Section: Discussionmentioning

confidence: 99%

Development and Application of Fruit Color-Related Expressed Sequence Tag-Simple Sequence Repeat Markers in Abelmoschus esculentus on the Basis of Transcriptome Sequencing

Luo

Liu

et al. 2022

Front. Plant Sci.

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Defining autopolyploidy: Cytology, genetics, and taxonomy

Lv,

Addo Nyarko,

Ramtekey

et al. 2024

American J of Botany

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Autopolyploidy is taxonomically defined as the presence of more than two copies of each genome within an organism or species, where the genomes present must all originate within the same species. Alternatively, “genetic” or “cytological” autopolyploidy is defined by polysomic inheritance: random pairing and segregation of the four (or more) homologous chromosomes present, with no preferential pairing partners. In this review, we provide an overview of methods used to categorize species as taxonomic and cytological autopolyploids, including both modern and obsolete cytological methods, marker‐segregation‐based and genomics methods. Subsequently, we also investigated how frequently polysomic inheritance has been reliably documented in autopolyploids. Pure or predominantly polysomic inheritance was documented in 39 of 43 putative autopolyploid species where inheritance data was available (91%) and in seven of eight synthetic autopolyploids, with several cases of more mixed inheritance within species. We found no clear cases of autopolyploids with disomic inheritance, which was likely a function of our search methodology. Interestingly, we found seven species with purely polysomic inheritance and another five species with partial or predominant polysomic inheritance that appear to be taxonomic allopolyploids. Our results suggest that observations of polysomic inheritance can lead to relabeling of taxonomically allopolyploid species as autopolyploid and highlight the need for further cytogenetic and genomic investigation into polyploid origins and inheritance types.

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Development of EST-based methylation specific PCR (MSP) markers in Crocus sativus

et al. 2022

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Evidence for hexasomic inheritance in Chrysanthemum morifolium Ramat. based on analysis of EST-SSR markers

Cited by 5 publications

References 34 publications

Development and Application of Fruit Color-Related Expressed Sequence Tag-Simple Sequence Repeat Markers in Abelmoschus esculentus on the Basis of Transcriptome Sequencing

Development and Application of Fruit Color-Related Expressed Sequence Tag-Simple Sequence Repeat Markers in Abelmoschus esculentus on the Basis of Transcriptome Sequencing

Defining autopolyploidy: Cytology, genetics, and taxonomy

Development of EST-based methylation specific PCR (MSP) markers in Crocus sativus

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