Evidence for pyrimidine-pyrimidine cyclobutane dimer formation in the covalent cross linking between transfer ribonucleic acid and 16S ribonucleic acid at the ribosomal P site

Ofengand, James; Liou, Richard

doi:10.1021/bi00562a016

Cited by 36 publications

(40 citation statements)

References 35 publications

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“…The results of this work support the proposed mechanism for tRNA and rRNA photo cross-linking in view of possible cyclobutane dimer formation between cytosine and 5-methoxyuracil residues (1)(2)(3)(4)(5). It might be surprising that no deamination of the photoproduct was observed in cross-linked tRNA-rRNA complexes except for the very recent report on photo cross-linking of E. coli acetylvalyl tRNA and Artemia salina ribosomes, in which some conversion of cytosine into uracil was detected (13).…”

Section: Resultssupporting

confidence: 84%

“…It has been found recently that complexes of certain tRNAs with Escherichia coli or yeast ribosomes could be cross-linked upon irradiation with near uv light (A > 300nm) (1)(2)(3)(4)(5). This cross-linking results in covalent bond formation between the tRNA 5'-anticodon base 5-methoxy or (5-carboxymethoxy) uracil and cytosine-1400 of the rRNA (5).…”

Section: Introductionmentioning

confidence: 99%

“…This cross-linking results in covalent bond formation between the tRNA 5'-anticodon base 5-methoxy or (5-carboxymethoxy) uracil and cytosine-1400 of the rRNA (5). Based on the photoreversal of the cross-link by 254-nm light, the cyclobutane-type dimer was proposed as the structure of the photoproduct (2).…”

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

Photocycloaddition of cytosine to 5-methoxyuracil in dinucleotide model compound

Skalski¹,

Wenska²,

Paszỳc³

et al. 1988

Can. J. Chem.

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The intramolecular photocycloaddition of cytosine (Cyt) to 5-methoxyuracil (mo5U) in Cyt-(CH2)3-mo5Ura, a dinucleotide model compound, occurs upon irradiation with near ultraviolet light (λ > 300 nm) with the formation of an unstable cyclobutane dimer. The dimer undergoes spontaneous dark deamination with a rate constant k = 9.0 × 10−5 s−1 and t1/2 = 128 min, to give a related uracil derivative.

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Section: Resultssupporting

confidence: 84%

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Photocycloaddition of cytosine to 5-methoxyuracil in dinucleotide model compound

Skalski¹,

Wenska²,

Paszỳc³

et al. 1988

Can. J. Chem.

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“…Perspective view was generated on the basis of molecular parameters given in refs. 27-29. photoreversibility (4). Cyclobutane dimerization would be facilitated if cmo5U3 of tRNAl"' were stacked with C14w of the 16S RNA at the P site prior to irradiation (22).…”

Section: Resultsmentioning

confidence: 99%

“…In the case ofE. coli tRNAY1'1, 254-nm irradiation resulted in true photoreversal ofthe RNA-RNA bond formation (4). On the basis of this and.…”

mentioning

confidence: 84%

Covalent crosslinking of tRNA1Val to 16S RNA at the ribosomal P site: identification of crosslinked residues.

Prince¹,

Taylor²,

Thurlow³

et al. 1982

Proc. Natl. Acad. Sci. U.S.A.

200

107

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N-Acetylvalyl-tRNAYl (AcVal-tRNA 1) was bound to the P site of uniformly 32P-labeled 70S ribosomes from Escherichia coli and crosslinked to 16S RNA in the 30S ribosomal subunit by irradiation with light of300-400 nm. To identify the crosslinked nucleotide in 16S RNA, AcVal-tRNAY. l-16S [32P]RNA was digested completely with RNase TI and the band containing the covalently attached oligonucleotides from tRNA and rRNA was isolated by polyacrylamide gel electrophoresis. The crosslinked oligonucleotide, and the 32P-labeled rRNA moiety released from it by photoreversal of the crosslink at 254 nm, were then analyzed by secondary hydrolysis with pancreatic RNase A and RNase U2. The oligonucleotide derived from 16S RNA was found to be the evolutionarily conserved sequence, U-A-C-A-C-A-C-C-G1401, and the nucleotide crosslinked to tRNAI , C14W. The identity of the covalently attached residue in the tRNA was established by using AcVal-tRNAlv-16S RNA prepared from unlabeled ribosomes.This complex was digested to completion with RNase T1 and the resulting RNA fragments were labeled at the 3' end with [5'-32P]pCp. The crosslinked Ti oligonucleotide isolated from the mixture yielded one major end-labeled component upon photoreversal. Chemical sequence analysis demonstrated that this product was derived from the anticodon-containing pentadecanucleo-

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Initiation factor 3-induced structural changes in the 30 s ribosomal subunit and in complexes containing tRNA f Met and mRNA 1 1Edited by D. E. Diaper

Shapkina

Dolan

Babin

et al. 2000

Journal of Molecular Biology

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Evidence for pyrimidine-pyrimidine cyclobutane dimer formation in the covalent cross linking between transfer ribonucleic acid and 16S ribonucleic acid at the ribosomal P site

Cited by 36 publications

References 35 publications

Photocycloaddition of cytosine to 5-methoxyuracil in dinucleotide model compound

Photocycloaddition of cytosine to 5-methoxyuracil in dinucleotide model compound

Covalent crosslinking of tRNA1Val to 16S RNA at the ribosomal P site: identification of crosslinked residues.

Initiation factor 3-induced structural changes in the 30 s ribosomal subunit and in complexes containing tRNA f Met and mRNA 1 1Edited by D. E. Diaper

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