Evidence for vasopressin production in the human gastrointestinal system

Friedmann, Andrew S.; Memoli, Vincent A.; North, William G.

doi:10.1016/0196-9781(91)90059-x

Cited by 11 publications

(7 citation statements)

References 14 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Interestingly, like many other neuropeptides, AVP production and release is not restricted to the hypothalamic neurohypophysial system. Indeed, AVP immunoreactivity and AVP-associated neurophysin were localized to crypt cells of rodent and human small intestine, suggesting that in addition to its well-known role as an endocrine hormone produced by hypothalamic neurons, AVP can also act as a paracrine signaling peptide in the intestine (24,25,69).…”

Section: Discussionmentioning

confidence: 99%

“…In the human gastrointestinal tract, earlier studies demonstrated the presence of vasopressin in crypt cells of the stomach and small intestine and in mononuclear cells within the lamina propria and submucosa as revealed by immunohistochemical examination (24). More recently, AVP-containing cells have been reported to be present in rat small and large intestines (35), as well as in a subpopulation of enteric neurons in the submucosal and myenteric plexuses of the canine colon (82).…”

mentioning

confidence: 97%

See 1 more Smart Citation

Vasopressin-mediated mitogenic signaling in intestinal epithelial cells

Chiu

Santiskulvong

et al. 2002

American Journal of Physiology-Cell Physiology

View full text Add to dashboard Cite

Here, we demonstrate that arginine vasopressin (AVP) induces multiple intracellular signal transduction pathways in rat intestinal epithelial IEC-18 cells via a V 1A receptor. Addition of AVP to these cells induces a rapid and transient increase in cytosolic Ca 2ϩ concentration and promotes protein kinase D (PKD) activation through a protein kinase C (PKC)-dependent pathway, as revealed by in vitro kinase assays and immunoblotting with an antibody that recognizes autophosphorylated PKD at Ser 916 . AVP also stimulates the tyrosine phosphorylation of the nonreceptor tyrosine kinase proline-rich tyrosine kinase 2 (Pyk2) and promotes Src family kinase phosphorylation at Tyr 418 , indicative of Src activation. AVP induces extracellular signal-related kinase (ERK)-1 (p44 mapk ) and ERK-2 (p42 mapk ) activation, a response prevented by treatment with mitogen-activated protein kinase kinase (MEK) inhibitors (PD-98059 and U-0126), specific PKC inhibitors (GF-I and Ro-31-8220), depletion of Ca 2ϩ (EGTA and thapsigargin), selective epidermal growth factor receptor (EGFR) tyrosine kinase inhibitors (tyrphostin AG-1478, compound 56), or the selective Src family kinase inhibitor PP-2. Furthermore, AVP acts as a potent growth factor for IEC-18 cells, inducing DNA synthesis and cell proliferation through ERK-, Ca 2ϩ -, PKC-, EGFR tyrosine kinase-, and Src-dependent pathways. arginine vasopressin; protein kinase D; protein kinase C; Src; proline-rich tyrosine kinase 2; intestinal epithelial proliferation THE SEQUENTIAL PROLIFERATION, lineage-specific differentiation, migration, and cell death of epithelial cells of the intestinal mucosa is a tightly regulated process that is modulated by a broad spectrum of regulatory peptides (8,36,70). The nontransformed IEC-6 and IEC-18 cells, derived from rat small intestinal crypt (56), have provided an in vitro model to examine intestinal epithelial cell migration, differentiation, and proliferation (16,27,58,70). Previous studies demonstrated that the proliferation and migration of these intestinal epithelial cells is regulated by a variety of polypeptide growth factors, including epidermal growth factor (EGF), insulin-like growth factor I, and hepatocyte growth factor, which act via single-pass transmembrane tyrosine kinase receptors (3,17,51). Neuropeptides and vasoactive peptides that signal through G protein-coupled receptors (GPCRs), characterized by seven-transmembrane helices, also act as potent cellular growth factors for a variety of cell types (63,64,66,67). However, the role of GPCRs and their ligands in intestinal epithelial cell signaling and proliferation remains poorly understood.The neurohypophysial nonapeptide arginine vasopressin (AVP), also known as antidiuretic hormone, is traditionally recognized for its role as a vasoconstrictor hormone acting on vascular smooth muscle cells and its antidiuretic effect via the renal collecting system. In addition to its function in the regulation of body fluid osmolality, vascular tone, and blood pressure, AVP acts as a growth-promo...

show abstract

Section: Discussionmentioning

confidence: 99%

mentioning

confidence: 97%

Vasopressin-mediated mitogenic signaling in intestinal epithelial cells

Chiu

Santiskulvong

et al. 2002

American Journal of Physiology-Cell Physiology

View full text Add to dashboard Cite

show abstract

“…Note that in this condition, the contractile response to AVP was abolished. Indeed AVP can be produced locally in cells of human and rat gastrointestinal system [33] and AVP receptors are reported to be expressed throughout gastrointestinal tract [6,24]. However, there are contradictory reports of the effects of vasopressin being different the responses in the various parts of the GI tract and among species.…”

Section: +mentioning

confidence: 97%

Arginine vasopressin, via activation of post-junctional V1 receptors, induces contractile effects in mouse distal colon

Mastropaolo

Zizzo

Auteri

et al. 2013

Regulatory Peptides

View full text Add to dashboard Cite

The aim of this study was to analyze whether arginine vasopressin (AVP) may be considered a modulator of intestinal motility. In this view, we evaluated, in vitro, the effects induced by exogenous administration of AVP on the contractility of mouse distal colon, the subtype(s) of receptor(s) activated and the action mechanism. Isometric recordings were performed on longitudinal and circular muscle strips of mouse distal colon. AVP (0.001 nM-100 nM) caused concentration-dependent contractile effects only on the longitudinal muscle, antagonized by the V1 receptor antagonist, V-1880. AVP-induced effect was not modified by tetrodotoxin, atropine and indomethacin. Contractile response to AVP was reduced in Ca 2+ -free solution or in the presence of nifedipine, and it was abolished by depletion of calcium intracellular stores after repetitive addition of carbachol in calcium-free medium with addition of cyclopiazonic acid. U-73122, an inhibitor of the phospholipase C, effectively antagonized AVP effects, whilst it was not affected by an adenylyl cyclase inhibitor. Oxytocin induced an excitatory effect in the longitudinal muscle of distal colon at very high concentrations, effect antagonized by V-1880. The results of this study shown that AVP, via activation of V1 receptors, is able to modulate positively contractile activity of longitudinal muscle of mouse distal colon, independently by enteric nerve activation and prostaglandin synthesis. Contractile response is achieved by increase in cytoplasmatic Ca 2+ concentration via extracellular Ca 2+ influx from L-type Ca 2+ channels and via Ca 2+ release from intracellular stores through phospholipase C pathway.No modulation has been observed on the contractility of the circular muscle.

show abstract

“…There was no evidence of proAVP expression with the remaining human tissue specimens, representing 65 various tissue types, contained on the microarray. The presence of AVP-related protein products in select cells of various tissues such as the thymus, spleen, and of the gastrointestinal system has been demonstrated 20–22. In other tissues where AVP-related immunoreactivity has been found, such as the testes, adrenal gland, and corpus luteum, there is question as to the whether the hormone is actually synthesized at that site 23–26.…”

Section: Discussionmentioning

confidence: 99%

Detection of Provasopressin in Invasive and Non-invasive (DCIS) Human Breast Cancer Using a Monoclonal Antibody Directed against the C-terminus (MAG1)

Keegan¹,

Memoli²,

Wells³

et al. 2010

Breast Cancer�(Auckl)

Self Cite

View full text Add to dashboard Cite

Abstract:The provasopressin protein (proAVP) is expressed by invasive breast cancer and non-invasive breast cancer, or ductal carcinoma in situ (DCIS). Here we demonstrate the ability of the monoclonal antibody MAG1 directed against the C-terminal end of proAVP to identify proAVP in all cases examined of human invasive cancer and DCIS (35 and 26, respectively). Tissues were chosen to represent a relevant variation in tumor type, grade, patient age, and menopausal status. By comparison, there was 65% positive staining for estrogen receptor, 61% for progesterone receptor, 67% for nuclear p53, and 39% for c-Erb-B2 with the invasive breast cancer sections. Reaction with the normal tissue types examined (67) was restricted to the vasopressinergic magnocellular neurons of the hypothalamus, where provasopressin is normally produced, and the posterior pituitary, where these neurons terminate. The breast epithelial tissue sections on the tissue microarray did not react with MAG1. Previously, we demonstrated that polyclonal antibodies to proAVP detected that protein in all breast cancer samples examined, but there was no reaction with breast tissue containing fibrocystic disease. The results presented here not only expand upon those earlier results, but they also demonstrate the specificity and effectiveness of what may be considered a more clinically-relevant agent. Thus, proAVP appears to be an attractive target for the detection of invasive breast cancer and DCIS, and these results suggest that MAG1 may be a beneficial tool for use in the development of such strategies.Keywords: invasive breast cancer, ductal carcinoma in situ (DCIS), vasopressin, provasopressin, monoclonal antibody Keegan et al

show abstract

Evidence for vasopressin production in the human gastrointestinal system

Cited by 11 publications

References 14 publications

Vasopressin-mediated mitogenic signaling in intestinal epithelial cells

Vasopressin-mediated mitogenic signaling in intestinal epithelial cells

Arginine vasopressin, via activation of post-junctional V1 receptors, induces contractile effects in mouse distal colon

Detection of Provasopressin in Invasive and Non-invasive (DCIS) Human Breast Cancer Using a Monoclonal Antibody Directed against the C-terminus (MAG1)

Contact Info

Product

Resources

About