Evidence of radicals created by plasma in bacteria in water

Lee, Chae Bok; Na, Young Ho; Hong, Tae-Eun; Choi, Eun Ha; Uhm, Han S.; Baik, Ku Youn; Kwon, Gi-Chung

doi:10.1063/1.4893565

Cited by 14 publications

(9 citation statements)

References 29 publications

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“…The metastable state of N 2 (A 3 u S + ) can generate OH radicals easily according to N 2 (A 3 u S + )+H 2 O→OH+H+N 2 with a high dissociation coefficient of α OH =5×10 −14 cm 3 s −1 [19]. Although the lengths of the plasma plume are short with this vapor ratio, the peaks of OH/OD are the highest, as shown in a previous study [14]. Therefore, gases with a ratio of 1.16% H 2 O in N 2 and 0.75% D 2 O in N 2 are used to increase the OH and OD radical effects in all of the following experiments.…”

Section: Nanosims Analysissupporting

confidence: 59%

“…The NanoSIMS image shows that deuterium is incorporated in both the cytoplasm and membrane, which are colocalized well with nitrogen and phosphorus atoms in E.coli. This is the first direct and visual evidence of OH radical effects on cell components comparing to other previous reports using NanoSIMS [13][14][15]. The new method involving the use of OD radicals instead of OH radicals through a plasma jet can be used to elucidate the inter-cellular mechanisms of OH radicals.…”

Section: Introductionmentioning

confidence: 57%

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Traces of isotopic reactive species produced from a non-thermal plasma jet in bio-molecules

et al. 2015

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Heavy water (D 2 O) is introduced into a non-thermal plasma jet (NTPJ) device to generate deuterium monoxide (OD) radicals instead of hydroxyl (OH) radicals. An NTPJ generated from a vapor mixture of N 2 /H 2 O and N 2 /D 2 O is applied to a cell membrane component and its effects are analyzed by means of 1 H NMR, GC-FID and TOF-SIMS spectroscopies. The results show that OH and OD radical species induce similar levels of oxidative breakage of lipid molecules. In addition, the 2 H NMR spectra show that deuteriums are incorporated into the lipid oxidative products. In order to trace these effects in vivo, E. coli bacteria are treated with an NTPJ and analyzed using NanoSIMS. Deuterium is observed in both the cytoplasm and membrane, which are colocalized well with nitrogen and phosphorus atoms. The high colocalization of D atoms inside E. coli provides the first direct and visual evidence of the role of OD radicals, which may be utilized to visualize OH radical interactions inside cells.

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Section: Nanosims Analysissupporting

confidence: 59%

Section: Introductionmentioning

confidence: 57%

Traces of isotopic reactive species produced from a non-thermal plasma jet in bio-molecules

et al. 2015

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“…A nonthermal plasma device is operated at atmospheric pressure inside a vacuum chamber filled with N 2 gas 41 . The plasma jet system is powered by a 60 Hz AC power supply using a neon transformer (PNP-1000, Daekwang Electric Co.).…”

Section: Methodsmentioning

confidence: 99%

Influence of oxygen on generation of reactive chemicals from nitrogen plasma jet

Uhm

Baik

et al. 2018

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A nonthermal plasma jet is operated at atmospheric pressure inside a vacuum chamber filled with nitrogen gas. Various chemical compounds are fabricated from nitrogen and water molecules in plasma jet with varying oxygen content. Detailed theoretical investigation of these chemical compounds is carried out in terms of different oxygen ratio ξ. Experimental measurements are also carried out for comparison with theoretical results. Hydroxyl molecules are mostly generated at surface of water, and some of them can penetrate into water. The density of hydroxyl molecules has its maximum without oxygen, and decreases to zero as ξ increases to 0.25. The density of the ammonia of NH3 also deceases as ξ increases to 0.25. On the other hand, theory and experiment show that the density of the NO3 increases drastically as ξ increases to 0.25. The hydrogen peroxide density in plasma activated water deceases, reaches its minimum value at ξ = 0.05, and then increases again, as ξ increases from a small value to a large value. The pH value of the plasma activated water, which is slightly changed to alkali without oxygen, decreases as ξ increases.

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“…1B) made of stainless steel (1.3 mm in inner diameter and 0.3 mm thickness). Plasma was generated in the porous alumina-based ceramics between the electrodes, which were separated by a gap of 1 mm [19]. Nitrogen was injected into the electrode and ejected through the 1-mm hole in the outer electrode via the porous layers.…”

Section: Non-thermal Plasma Jet (Ntpj) Devicementioning

confidence: 99%

“…Optical spectroscopy of the discharge was performed using a spectrometer (Ocean Optics, HR4000, USA) at 0.7 nm resolution. The detailed design and function of NTPJ has been previously reported [19].…”

Section: Non-thermal Plasma Jet (Ntpj) Devicementioning

confidence: 99%

Inactivation of Mycobacteria by Radicals from Non-Thermal Plasma Jet

Lee¹,

Subhadra²,

Choi³

et al. 2019

Journal of Microbiology and Biotechnology

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Mycobacterial cell walls comprise thick and diverse lipids and glycolipids that act as a permeability barrier to antibiotics or other chemical agents. The use of OH radicals from a non-thermal plasma jet (NTPJ) for the inactivation of mycobacteria in aqueous solution was adopted as a novel approach. Addition of water vapor in a nitrogen plasma jet generated OH radicals, which converted to hydrogen peroxide (H 2 O 2) that inactivated non-pathogenic Mycobacterium smegmatis and pathogenic Mycobacterium tuberculosis H37Rv. A stable plasma plume was obtained from a nitrogen plasma jet with 1.91 W of power, killing Escherichia coli and mycobacteria effectively, whereas addition of catalase decreased the effects of the former. Mycobacteria were more resistant than E. coli to NTPJ treatment. Plasma treatment enhanced intracellular ROS production and upregulation of genes related to ROS stress responses (thiolrelated oxidoreductases, such as SseA and DoxX, and ferric uptake regulator furA). Morphological changes of M. smegmatis and M. tuberculosis H37Rv were observed after 5 min treatment with N 2 +H 2 O plasma, but not of pre-incubated sample with catalase. This finding indicates that the bactericidal efficacy of NTPJ is related to the toxicity of OH and H 2 O 2 radicals in cells. Therefore, our study suggests that NTPJ treatment may effectively control pulmonary infections caused by M. tuberculosis and nontuberculous mycobacteria (NTM) such as M. avium or M. abscessus in water.

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Evidence of radicals created by plasma in bacteria in water

Cited by 14 publications

References 29 publications

Traces of isotopic reactive species produced from a non-thermal plasma jet in bio-molecules

Traces of isotopic reactive species produced from a non-thermal plasma jet in bio-molecules

Influence of oxygen on generation of reactive chemicals from nitrogen plasma jet

Inactivation of Mycobacteria by Radicals from Non-Thermal Plasma Jet

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