Evidence that ‘Inactive’ Renin is Produced outside the Kidney of the Rat

Vandongen, R.; Poessée, Marianne; Strang, K. D.; Birkenhäger, W. H.

doi:10.1042/cs0530189

Cited by 13 publications

(10 citation statements)

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“…This compares closely with the average value of 15% reported by Vandongen et al (1977) for the rat but is substantially less than the 62% in normal human plasma found by Skinner, Cran, Gibson, Taylor, Walters & Catt (1975). Similar values to these were also reported by Leckie et al (1977) and Boyd (1977).…”

Section: Discussionsupporting

confidence: 87%

Inactive Renin in Rabbit Plasma: Effect of Haemorrhage

et al. 1979

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1. Renin activity in rabbit plasma increases after acidification (pH 3.3), probably due to activation of an inactive form of renin. 2. Both active and inactive renin in plasma increase after haemorrhage. This stimulus does not change the relative proportions of the two forms. 3. After ligation of the renal blood vessels neither form of renin increases in response to haemorrhage. 4. One day after bilateral nephrectomy no inactive renin could be demonstrated in plasma. 5. In the rabbit, therefore, the kidney is a major source of the inactive renin in plasma.

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Section: Discussionsupporting

confidence: 87%

Inactive Renin in Rabbit Plasma: Effect of Haemorrhage

et al. 1979

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“…However, the number of observations made by Morris & Johnston (1976) was limited (n = 2), and since renin recovery during dialysis was not measured by either team, it is not possible to conclude that there was in fact an increase in renin concentration in these studies. The small (15%) increase in renin concentration after acidification reported by Vandongen et al (1977) may well have resulted from differences in renin recovery at different pH, as demonstrated in our experiments. Contrary to the hypothesis of Vandongen et al (1977), we did not find inactive renin of extrarenal origin in our experiments with binephrectomized rat plasma dialysed to an acid pH.…”

Section: Discussioncontrasting

confidence: 43%

“…It may be worth noting that the methods of estimation applied by some workers tend to overlook the influence of renin inactivation by plasma, which may lead to overestimation of acid-activatable renin. The presence of inactive renin in rat plasma has been reported by Morris & Johnston (1976) and Vandongen et al (1977). These authors observed higher renin values in aciddialysed rat plasma compared with those in control plasma dialysed to a less acid pH.…”

Section: Discussionmentioning

confidence: 71%

Effect of Acid, Trypsin and Cold Treatment and of Renin— Plasma Interaction on the Activity of Renin Secreted by Rat Kidney

Nakane¹,

Nakane²,

Corvol³

et al. 1979

Clinical Science

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1. Renin release from the isolated perfused rat kidney was markedly stimulated by isoprenaline or anoxia. Renin secreted into the blood-free perfusate was not activated by exposure to cold or dialysis to pH 3.3, suggesting the absence either of cryo-or acid-activatable renin or of factors necessary to activate inactive renin.2. Trypsin treatment did not change renin concentration in the perfusate samples.3. When binephrectomized rat plasma was added to perfusate samples before dialysis, renin concentration in the acidified samples was significantly higher than in samples dialysed to pH 6.5. Diminished renin recovery in the latter samples caused this difference. Binephrectomized rat plasma itself had no significant renin activity before or after acid dialysis, indicating the absence of any important extrarenal source of active or acid-activatable renin in rats.4. Acidification of binephrectomized rat plasma before its addition to the perfusate samples markedly reduced the difference between renin recovery during dialysis to pH 3.3 and dialysis to pH 6.5, indicating that acidification irreversibly inhibited renin inactivation by binephrectomized rat plasma. No net increase in renin concentration was observed in any of our experiments.5. These results suggest that rat kidney does not secrete inactive renin. They also point to the existence of renin inactivation by rat plasma at Correspondence: Dr Haruyuki Nakane, INSERM U36, 17, rue du Fer-a-Moulin, 75005 Paris, France. neutral pH, which might lead to overestimation of acid-activatable renin in rats.

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“…Inactive renin may be released from the kidney directly (Derkx et al 1976;Birkenhager et al 1978;Millar et al 1978;Derkx et al 1983), or active renin may be converted to inactive form in the circulation (Vandongen et al 1977;Aoi et al 1979Aoi et al , 1981. But in any case, it seems reasonable to assume that TRC, i.e.…”

Section: Discussionmentioning

confidence: 99%