Evolution and phylogenetic information content of the ribosomal DNA repeat unit in the Blattodea (Insecta)

Муха, Д. В.; Wiegmann, Brian M.; Schal, Coby

doi:10.1016/s0965-1748(01)00164-3

Cited by 28 publications

(27 citation statements)

References 32 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The amplified rDNA regions contained the ITS1 (261–388 bp) and ITS2 (188–262 bp) regions, the 5.8S gene (157 bp), and approximately 200 and 1000 bp of the 18S and 28s genes, respectively. The amplification was performed in 50 μ L reactions using a PCR Master Mix (2X) (Fermentas, Vilnius, Lithuania) according to the manufacturer's instructions; the reactions were performed in a Primus 25 advanced Thermocycler (PEQLAB, Erlangen, Germany) using previously published rDNA-specific parameters [98]. The PCR products were resolved on 1.0% agarose gels, and DNA was extracted from the observed unique bands using the QIAquick Gel Extraction Kit (QIAGEN, Hilden, Germany).…”

Section: Methodsmentioning

confidence: 99%

Phylogenetic Information Content of Copepoda Ribosomal DNA Repeat Units: ITS1 and ITS2 Impact

Zagoskin

Лазарева

Grishanin

et al. 2014

BioMed Research International

View full text Add to dashboard Cite

The utility of various regions of the ribosomal repeat unit for phylogenetic analysis was examined in 16 species representing four families, nine genera, and two orders of the subclass Copepoda (Crustacea). Fragments approximately 2000 bp in length containing the ribosomal DNA (rDNA) 18S and 28S gene fragments, the 5.8S gene, and the internal transcribed spacer regions I and II (ITS1 and ITS2) were amplified and analyzed. The DAMBE (Data Analysis in Molecular Biology and Evolution) software was used to analyze the saturation of nucleotide substitutions; this test revealed the suitability of both the 28S gene fragment and the ITS1/ITS2 rDNA regions for the reconstruction of phylogenetic trees. Distance (minimum evolution) and probabilistic (maximum likelihood, Bayesian) analyses of the data revealed that the 28S rDNA and the ITS1 and ITS2 regions are informative markers for inferring phylogenetic relationships among families of copepods and within the Cyclopidae family and associated genera. Split-graph analysis of concatenated ITS1/ITS2 rDNA regions of cyclopoid copepods suggested that the Mesocyclops, Thermocyclops, and Macrocyclops genera share complex evolutionary relationships. This study revealed that the ITS1 and ITS2 regions potentially represent different phylogenetic signals.

show abstract

Section: Methodsmentioning

confidence: 99%

Phylogenetic Information Content of Copepoda Ribosomal DNA Repeat Units: ITS1 and ITS2 Impact

Zagoskin

Лазарева

Grishanin

et al. 2014

BioMed Research International

View full text Add to dashboard Cite

show abstract

“…Use of PCR as a tool to amplify and sequence genes and then exploit the nucleotide data for phylogenetic analysis and develop evolutionary relationships among grasshopper species has previously been practiced by a number of researchers (Colgan 1991;Chapco & Litzenberger 2003;Rowell & Flook 2004). Several researchers have used DNA data in phylogenetic analysis to identify grasshopper species (Chapco & Litzenberger 2002;Mukha et al 2001;Song & Wenzel (2007) Ketmaier et al 2010). Use of DNA data has also been used in combination with morphological data to establish species relationships (Brust 2008 Kirby (1914), Fletcher (1919, Mahmood (1995), Mahmood & Yousaf (1999), Mahmood & Yousaf (2000); ; Azim & Reshi (2010) but nobody has made any effort to identify them on a molecular level either by DNA barcoding or by using any other marker.…”

Section: Introductionmentioning

confidence: 99%

Morphological and molecular identification of acridid grasshoppers (Acrididae: Orthoptera) from Poonch division, Azad Jammu Kashmir, Pakistan

Nazir¹,

Mehmood²,

Ashfaq³

et al. 2014

J. Threat. Taxa

View full text Add to dashboard Cite

“…On the other hand, comparisons among ribosomal DNA internal transcribed spacer (rDNA ITS) sequences have been helpful to ascertain evolutionary relatedness of closely related species in diverse insect groups, owing to their high evolutionary rates (Vogler and DeSalle, 1994;Schlötterer et al, 1994: Di Muccio et al, 2000Weekers et al, 2001, Mukha et al, 2002. Recently, studies of Coleoptera and Hemiptera demonstrated the usefulness of ITS-1 and ITS-2 markers in discovering phylogenetic relationships at higher levels such as tribe and subfamily (Marcilla et al, 2001;Von der Schulenburg, 2001).…”

Section: Introductionmentioning

confidence: 99%

The first internal transcribed spacer (ITS-1) of Melipona species (Hymenoptera, Apidae, Meliponini): characterization and phylogenetic analysis

et al. 2005

View full text Add to dashboard Cite

Internal transcribed spacer 1 (ITS-1) sequences of the nuclear rDNA of eight bee species of the genus Melipona were studied. Complete ITS-1 sequence and flanking regions from three Melipona species were PCR-amplified, cloned, sequenced, and their variability compared. These sequences show length variation (1391 to 1417 bp), several repeated elements of one, two, three, and four nucleotides, and a repeated tandem sequence of approximately 80 bp. The low variation level between M. quadrifasciata and M. mandacaia sequences supports the hypothesis that they diverged recently. PCR-amplification, cloning, and sequencing of a partial ITS-1 sequence (394 to 496 bp) of eight Melipona species and two outgroups were performed and the obtained sequences used for phylogenetic analysis. The single tree estimated from parsimony analysis recovered four well-defined clades and monophyly of the genus Melipona. The phylogenetic relationships derived from sequences of ITS-1 fragments corroborate the taxonomic classification of Melipona based on morphological characters.

show abstract

Evolution and phylogenetic information content of the ribosomal DNA repeat unit in the Blattodea (Insecta)

Cited by 28 publications

References 32 publications

Phylogenetic Information Content of Copepoda Ribosomal DNA Repeat Units: ITS1 and ITS2 Impact

Phylogenetic Information Content of Copepoda Ribosomal DNA Repeat Units: ITS1 and ITS2 Impact

Morphological and molecular identification of acridid grasshoppers (Acrididae: Orthoptera) from Poonch division, Azad Jammu Kashmir, Pakistan

The first internal transcribed spacer (ITS-1) of Melipona species (Hymenoptera, Apidae, Meliponini): characterization and phylogenetic analysis

Contact Info

Product

Resources

About