Evolutional imprints on the sequences of BMP2/4/DPP type proteins

Künnapuu, Jaana; Shimmi, Osamu

doi:10.4161/fly.4.1.10652

Cited by 7 publications

(9 citation statements)

References 7 publications

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“…In contrast, Drosophila dpp induces bone formation in mammalian cells (Künnapuu and Shimmi, 2010;Sampath et al , 1993). Moreover, the ligands of BMP-2, BMP-4, and dpp have evolutionarily conserved roles in embryonic development (Künnapuu and Shimmi, 2010).…”

Section: Construction Of Mammalian Cell Expression Plasmidmentioning

confidence: 99%

“…Interestingly, the Drosophila dpp gene is functionally interchangeable with mammalian BMP-2 and BMP-4 (Chen et al, 1998). The hBMP-4 transgene can rescue dorsal embryonic pattern defects in Drosophila dppmutant flies (Künnapuu and Shimmi, 2010;Padgett et al , 1993). In contrast, Drosophila dpp induces bone formation in mammalian cells (Künnapuu and Shimmi, 2010;Sampath et al , 1993).…”

Section: Construction Of Mammalian Cell Expression Plasmidmentioning

confidence: 99%

“…The hBMP-4 transgene can rescue dorsal embryonic pattern defects in Drosophila dppmutant flies (Künnapuu and Shimmi, 2010;Padgett et al , 1993). In contrast, Drosophila dpp induces bone formation in mammalian cells (Künnapuu and Shimmi, 2010;Sampath et al , 1993). Moreover, the ligands of BMP-2, BMP-4, and dpp have evolutionarily conserved roles in embryonic development (Künnapuu and Shimmi, 2010).…”

Section: Construction Of Mammalian Cell Expression Plasmidmentioning

confidence: 99%

“…The TGF-β superfamily contains conserved polypeptide growth factors that play important roles in different cellular processes such as proliferation, apoptosis, differentiation and cell-fate determination (Chen et al , 1998;Künnapuu and Shimmi, 2010). Human bone morphogenetic proteins (hBMPs) constitute the largest subgroup of the TGF-β superfamily and have the same roles as Drosophila dpp (Chen et al, 1998;Künnapuu and Shimmi, 2010).…”

Section: Construction Of Mammalian Cell Expression Plasmidmentioning

confidence: 99%

“…The 1,285-bp full-length cDNA of B. mori dpp was amplified using primers (forward: Bm dpp-F and reverse: multiple developmental processes and belongs to the transforming growth factor beta (TGF-β) superfamily (Chen et al , 1998;Künnapuu and Shimmi, 2010). The TGF-β superfamily contains conserved polypeptide growth factors that play important roles in different cellular processes such as proliferation, apoptosis, differentiation and cell-fate determination (Chen et al , 1998;Künnapuu and Shimmi, 2010). Human bone morphogenetic proteins (hBMPs) constitute the largest subgroup of the TGF-β superfamily and have the same roles as Drosophila dpp (Chen et al, 1998;Künnapuu and Shimmi, 2010).…”

Section: Construction Of Mammalian Cell Expression Plasmidmentioning

confidence: 99%

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Functional analysis of Bombyx mori Decapentaplegic gene for bone differentiation in a mammalian cell

Park¹,

Goo²,

Choi³

et al. 2013

International Journal of Industrial Entomology

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Bone morphogenetic proteins (BMPs) belong to the transforming growth factor (TGF-b) superfamily and are involved in osteoblastic differentiation. The largest TGF-b superfamily subgroup shares genetic homology with human BMPs (hBMPs) and silkworm decapentaplegic (dpp). In addition, hBMPs are functionally interchangeable with Drosophila dpp . Bombyx mori dpp may induce bone formation in mammalian cells. To test this hypothesis, we synthesized the 1,285-base pairs cDNA of full-length B. mori dpp using total RNAs obtained from the fat body of 3-day-old of the 5 th instar larvae and cloned the cDNA into the pCEP4 mammalian expression vector. Next, B. mori dpp was expressed in C3H10T1/2 cells. The target cells transfected with the pCEP4-Bm dpp plasmid showed biological functions similar to those of osteogenic differentiation induction growth factors such as hBMPs. We determined the relative mRNA expression rates of Runt-related transcription factor 2 (RUNX2), osterix, osteocalcin, and alkaline phosphatase (ALP) to validate the osteoblast-specific differentiation effects of B. mori dpp by performing quantitative real-time RT-PCR. Interestingly, mRNA expression levels of the 3 marker genes except RUNX2, in cells expressing B. mori dpp were much higher than those in control cells and C3H10T1/2 cells transfected with pCEP4. These results suggested that B. mori dpp signaling regulates osterix expression during osteogenic differentiation via RUNX2-independent mechanisms.

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Section: Construction Of Mammalian Cell Expression Plasmidmentioning

confidence: 99%

Section: Construction Of Mammalian Cell Expression Plasmidmentioning

confidence: 99%

Section: Construction Of Mammalian Cell Expression Plasmidmentioning

confidence: 99%

Section: Construction Of Mammalian Cell Expression Plasmidmentioning

confidence: 99%

Section: Construction Of Mammalian Cell Expression Plasmidmentioning

confidence: 99%

See 3 more Smart Citations

Functional analysis of Bombyx mori Decapentaplegic gene for bone differentiation in a mammalian cell

Park¹,

Goo²,

Choi³

et al. 2013

International Journal of Industrial Entomology

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Silencing of Decapentaplegic (Dpp) gene inhibited the wing expansion in the white‐backed planthopper, Sogatella furcifera (Horváth) (Hemiptera: Delphacidae)

Long

Yang

Jin

et al. 2022

Arch Insect Biochem Physiol

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The Decapentaplegic gene controls wing patterning and spreading by regulating downstream genes in many insect species. However, the molecular characteristics, expression, and biological function of Dpp in Sogatella furcifera remain poorly understood. In this study, we cloned the Dpp gene from S. furcifera and examined its expression levels in different development stages, wing typed adults, and tissues. Then, the function of SfDpp gene was analyzed using an RNA interference (RNAi)‐based approach. The results showed that the full‐length complementary DNA of the SfDpp gene consists of 1034 bp and contains a 954‐bp open reading frame encoding 317 amino acids. SfDpp has a transforming growth factor‐β (TGF‐β) propeptide superfamily domain and a TGF‐β superfamily domain, typical of members of the TGF‐β superfamily. Quantitative real‐time polymerase chain reaction showed that the expression of SfDpp reached its highest expression level 40 min after eclosion. RNAi‐based gene silencing inhibited transcript levels of the corresponding messenger RNA in S. furcifera nymphs injected with double‐stranded RNA of SfDpp and resulted in death of 29.17% and 26.67% of 4th and 5th instar nymphs, respectively. The wing deformity rate of the adults was 74.12% and 3.41% after SfDpp gene silencing in 4th and 5th instar nymphs, respectively. Examining wing development‐associated genes showed that two target genes of Dpp (Vestigial and Spalt) were both dramatically downregulated after SfDpp was silenced. Our results demonstrate that downregulated SfDpp in early development causes wing expansion failure in S. furcifera. Thus, Dpp may be a target gene for restricting the migration of rice‐damaging planthoppers.

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A novel splice variant of the decapentaplegic (dpp) gene in the wild silkworm, Bombyx mandarina

Choi

Kim

Choi

et al. 2015

Biochemical and Biophysical Research Communications

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Evolutional imprints on the sequences of BMP2/4/DPP type proteins

Cited by 7 publications

References 7 publications

Functional analysis of Bombyx mori Decapentaplegic gene for bone differentiation in a mammalian cell

Functional analysis of Bombyx mori Decapentaplegic gene for bone differentiation in a mammalian cell

Silencing of Decapentaplegic (Dpp) gene inhibited the wing expansion in the white‐backed planthopper, Sogatella furcifera (Horváth) (Hemiptera: Delphacidae)

A novel splice variant of the decapentaplegic (dpp) gene in the wild silkworm, Bombyx mandarina

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