Ex Situ Conservation of Tropical Rare Fruit Species

Normah, M. N.; Clyde, M. M.; Cho, E.G.; Rao, V. Ramanatha

doi:10.17660/actahortic.2002.575.23

Cited by 11 publications

(14 citation statements)

References 3 publications

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“…Reviews of these studies are available (Normah and Marzalina 1996;Marzalina and Krishnapillay 1999;Normah et al 2002). The effects of moisture content and low temperature on seed survival provide the basis of investigations to determine freezing and desiccation tolerance of the seeds.…”

Section: Seed Recalcitrancementioning

confidence: 99%

In vitro conservation of Malaysian biodiversity—achievements, challenges and future directions

Noor

Kean

Yap

et al. 2010

In Vitro Cell.Dev.Biol.-Plant

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Malaysia is fortunate and proud to contain some of the world's richest biodiversity. In Malaysia, there are an estimated 185,000 species of fauna and 12,500 species of flowering plants, many of which are endemic to tropical forests in this region. Indeed, such diversity is an important and invaluable national asset to safeguard both present and future generations. In vitro conservation offers possible techniques for the preservation of plant germplasm that at present is difficult to maintain or is maintained with limited success. Research at the Universiti Kebangsaan Malaysia (The National University of Malaysia) focuses on the cryopreservation of woody fruit species with seeds that cannot tolerate cryopreservation (recalcitrant or intermediate).Among the plants with recalcitrant seeds are such traditionally important edible tropical fruits as mangosteen, langsat, and rambai (Garcinia mangostana, Lansium domesticum, and Baccaurea motleyana). Citrus aurantifolia, Citrus suhuiensis, Citrus madurensis, Citrus hystrix, and Fortunella polyandra are among the Citrus and Citrus-related species studied. Cryopreservation studies include the Nepenthes species (pitcher plants) of Malaysia. Fundamental research on desiccation and low-temperature tolerance and on the physiology of desiccation are used to understand seed behavior, a prerequisite for the development of successful conservation techniques. At the same time, cryopreservation protocols for several Citrus and forestry species were developed for embryonic axes and adventitious shoots, mainly using rapid dehydration and PVS2 vitrification techniques. There are no successful standard techniques or protocols for species with highly recalcitrant seeds such as Garcinia species. Modification of existing protocols or development of new methods is required, but this can be accomplished only when a detailed understanding of the recalcitrant nature of the seeds or explants is achieved. While we have considerable knowledge concerning the basics of biochemical processes and some molecular data from work on desiccation-tolerant seeds, a great need remains for understanding the cause of the recalcitrance or desiccation sensitivity of these seeds. It may be necessary to use a systems biology approach that exploits the "omics" technologies to generate global molecular data. In combination with bioinformatics for data integration and analyses, this approach would move toward improved modeling of the biological pathways associated with the development of recalcitrant seeds.

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Section: Seed Recalcitrancementioning

confidence: 99%

In vitro conservation of Malaysian biodiversity—achievements, challenges and future directions

Noor

Kean

Yap

et al. 2010

In Vitro Cell.Dev.Biol.-Plant

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“…Reducing the temperature is a common strategy used for tropical and subtropical species because they typically demonstrate significant and unique reductions in plant metabolism (Normah, Chin, & Reed, 2013). These type of responses have been observed in pineapple (Canto et al, 2004;Souza et al, 2006), cassava (Souza et al, 2009), sugarcane (Lemos, Ferreira, Alencar, Albuquerque, & Ramalho Neto, 2002), grapevine (Silva, Luis, & ScherwinskiPereira, 2012), banana (Oliveira, Silva, Silva, & Silveira, 2000) and potato (Conceição, Fortes, & Silva, 1999).…”

Section: Resultsmentioning

confidence: 99%

<b>Univariate and multivariate statistical tools for <i>in vitro</i> conservation of citrus genotypes

et al. 2016

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ABSTRACT. This study aimed to evaluate the influence of the growing environment on the in vitro conservation of citrus genotypes obtained from the Active Citrus Germplasm Bank of Embrapa Cassava and Fruit. The study used multivariate statistic tools in order to improve the efficiency in the analysis of the results. Approximately 1-cm of length microcuttings from plantlets derived from ten genotypes previously cultured in vitro were inoculated in test tubes containing 20 mL of WPM culture medium supplemented with 25 g L

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“…Currently, only about 100 accessions have been collected and conserved by various organizations, namely Laboratory of Biotechnology, Genetic Resources, Animal and Plant Breeding (Dassa-Zoumè, Benin), University of Development Studies (Tamale, Ghana), Gembloux Agricultural University (Belgium) and [Institut de Recherche pour le Développe-ment (IRD)] (Montpellier, France) [25,33,43]. This is a great limiting factor for the promotion, improvement of the crop productivity and its conservation since its cultivation is even threatened of extinction in some areas [24,27,33,64]. Investing in the discovery or the development of genetically superior varieties is a viable and important option for increasing productivity [65,66].…”

Section: Threats and Conservation Statusmentioning

confidence: 99%

Potential of Kersting’s groundnut [Macrotyloma geocarpum (Harms) Maréchal & Baudet] and prospects for its promotion

Ayenan

Ezin

2016

Agric & Food Secur

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Kersting's groundnut is a tropical crop, highly nutritious, adapted to drought-prone areas. The crop is neglected both by researchers and by policy makers. This paper aimed at providing relevant information on the current status of the crop and the prospects to promote its improvement and production. To this end, available papers addressing any of the following aspect: distribution, nutritional value, cropping systems, post-harvest processing, usages, value chains, conservation status, genetic diversity and improvement of Kersting's groundnut were reviewed. In West Africa, the crop provides substantial income for rural population. Kersting's groundnut has several medicinal uses and cultural values as well. However, because of its intensive labor requirement, low yield and non-availability of improved varieties, its cultivation is declining and it is even disappearing gradually in some growing areas. The promotion of the crop's value chains is an option for reversing the declining trend in Kersting's groundnut cultivation. In recent years, some progress has been recorded in the collection, characterization and ex situ conservation of the crop. Thus, there are currently about 100 accessions conserved in various gene banks in Benin, Ghana, France and Belgium. This is still insufficient as compared to the genetic resources available in ex situ for most of the grown legume crops. To cope with this, extensive germplasm collection and their systematic characterization and evaluation coupled with new generation genomic tools need to be undertaken. For this purpose, genomic resources developed for Kersting's groundnut-related species will be valuable assets. The exploitation of genomic resources will enable the development of core and mini-core collections for conservation and breeding purposes. In addition, the use of genomic resources will speed up Kersting's groundnut breeding programs. Furthermore, the genetic base of the crop is extremely narrow and there is a need to broaden it for substantial genetic gain in breeding programs. For this purpose, mutation induction has been proposed as technique to increase variability in the Kersting's groundnut germplasm. Invest in Kersting's groundnut research is key to ensure the conservation and exploit the potential of the crop.

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Ex Situ Conservation of Tropical Rare Fruit Species

Cited by 11 publications

References 3 publications

In vitro conservation of Malaysian biodiversity—achievements, challenges and future directions

In vitro conservation of Malaysian biodiversity—achievements, challenges and future directions

<b>Univariate and multivariate statistical tools for <i>in vitro</i> conservation of citrus genotypes

Potential of Kersting’s groundnut [Macrotyloma geocarpum (Harms) Maréchal & Baudet] and prospects for its promotion

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