Ex-Vivo Expanded Umbilical Cord Blood Stem Cells Retain Capacity for Myocardial Regeneration

Abstract

Background: Umbilical cord blood (UCB) is a source of human hematopoietic precursor cells (HPCs), a stem cell (SC) type that has been used in several trials for myocardial repair. A certain minimal number of cells is required for measurable regeneration and a major challenge of SC-based regenerative therapy constitutes exvivo expansion of the primitive cell compartment. The aim of this study was to investigate the ex-vivo expansion potential of UCB-derived HPCs and the ability of these expanded cells to migrate to the site of damage and improve ventricular function in a rodent model of myocardial infarction (MI). Methods and Results:UCB-derived HPCs, defined by coexpression of CD133 and CD34, were expanded using various cytokine combinations. MI was induced by left anterior descending artery ligation in nude rats. Cells were injected intravenously 2 days after infarction. The combination of SC factor, thrombopoietin, flt3-ligand and interleukin-6 was found to be the most effective for inducing proliferation of HPCs. The migratory capacity of expanded HPCs was similar to that of non-expanded HPCs and improvement of ejection fraction was significant in both groups, with a relative increase of >60%.Conclusions: UCB-derived HPCs can be reproducibly expanded ex-vivo and retain their potential to improve cardiac function post-MI. (Circ J 2010; 74: 188 - 194)

Development of Serum-Free Quality and Quantity Control Culture of Colony-Forming Endothelial Progenitor Cell for Vasculogenesis

“…These reports suggest that poor responses to cellular therapy are due to quantitative and/or qualitative impairment of the EPC fraction. To further augment the efficacy of EPC transplantation, several methodological approaches to enhance EPC bioactivities are currently being developed [5,[23][24][25][26][27][28].…”

“…In this regard, the several culture systems of suspended EPC phenotype have been developed and demonstrated the significance of EPC expansions ex vivo and the efficiency of cultured CD34 ϩ or CD133 ϩ cell transplant for neovascularization in ischemic animal models [5,[23][24][25][26][27][28].…”

<b>Development of equipment for decellularization using the perfusion method

“…Therefore, the field of tissue engineering has great potential in identifying new transplant strategies to provide better quality of life (Angiolillo & Goto, 2010;Limbourg, Limbourg, & Drexler, 2009;Shlechta et al, 2010). The creation of scaffolds that can be used for the development of complex three-dimensional tissues or whole organs remains a serious challenge in tissue engineering (Gálvez-Mónton, Prat-Vidal, Roura, Soler-Botija, & BayesGenis, 2013).…”

Carbon nanotube-based substrates promote cardiogenesis in brown adipose-derived stem cells via &beta;1-integrin-dependent TGF-&beta;1 signaling pathway

“…3,4 Thus far, it has been shown that various populations of stem cells, such as bone marrow mesenchymal stem cells, 5 white adipose-derived stem cells, 6 umbilical cord blood stem cells, 7 and resident cardiac stem cells, 8 are capable of producing CMs to inhibit further deterioration and help restore cardiac function caused by ischemic heart disease. However, the differentiation ability of these stem cells into CMs was assisted by the stimulation of chemical inducers.…”