Ex Vivo Exposure to Soft Biological Tissues by the 2-μm All-Fiber Ultrafast Holmium Laser System

Kopyeva, Mariya S.; Filatova, Serafima A.; Kamynin, V. A.; Trikshev, A. I.; Kozlikina, Elizaveta I.; Astashov, V. V.; Loschenov, Victor B.; Цветков, В. Б.

doi:10.3390/app12083825

Cited by 14 publications

(8 citation statements)

References 53 publications

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“…[ 4 ]. To remove biological tissues, various scenarios for the interaction of laser radiation with an object are used: ablation (direct removal of a substance); coagulation; welding (connection); and crushing (using a shock wave) [ 5 ]. Overall, the laser appears to be an exceptionally accurate, versatile and user-friendly tool and has a great potential for medical applications [ 6 ].…”

Section: Introductionmentioning

confidence: 99%

Effect of Laser-Induced Optical Breakdown on the Structure of Bsa Molecules in Aqueous Solutions: An Optical Study

et al. 2022

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The influence of laser radiation of a typical surgical laser on the physicochemical properties of the Bovine Serum Albumin (BSA) protein was studied. It was established that the physicochemical characteristics of optical breakdown weakly depend on the concentration of protein molecules. At the same time, the patterns observed for an aqueous solution of BSA irradiated with a laser for different time periods were extremely similar to the classical ones. It was established that after exposure to laser radiation, the optical density of protein solutions increases. At the same time, the intensity of BSA fluorescence due to aromatic amino acid residues decreases insignificantly after exposure to laser radiation. In this case, the position of the excitation and emission maximum does not change, and the shape of the fluorescence spot on 3D maps also does not change significantly. On the Raman spectrum after exposure to laser radiation, a significant decrease in 1570 cm−1 was observed, which indicates the degradation of α-helices and, as a result, partial denaturation of BSA molecules. Partial denaturation did not significantly change the total area of protein molecules, since the refractive index of solutions did not change significantly. However, in BSA solutions, after exposure to laser radiation, the viscosity increased, and the pseudoplasticity of aqueous solutions decreased. In this case, there was no massive damage to the polypeptide chain; on the contrary, when exposed to optical breakdown, intense aggregation was observed, while aggregates with a size of 400 nm or more appeared in the solution. Thus, under the action of optical breakdown induced by laser radiation in a BSA solution, the processes of partial denaturation and aggregation prevail, aromatic amino acid residues are damaged to a lesser extent, and fragmentation of protein molecules is not observed.

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Section: Introductionmentioning

confidence: 99%

Effect of Laser-Induced Optical Breakdown on the Structure of Bsa Molecules in Aqueous Solutions: An Optical Study

et al. 2022

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“…Pulsed fiber lasers of 2.1 µm have gained widespread interest due to their inherent advantages in a range of application fields, including free-space communication, mid-infrared nonlinear frequency conversion and so on [1][2][3][4] . For example, in free-space communication, a high repetition frequency (~GHz) pulsed laser in the 2.1 µm band exhibits more reliable data transmission than a short-wave high repetition frequency pulsed laser in extremely dense smoke conditions [1] .…”

Section: Introductionmentioning

confidence: 99%

2.1 m, high-energy dissipative soliton resonance from a holmium-doped fiber laser system

Zhao

Zhang²,

Zhu³

et al. 2023

High Pow Laser Sci Eng

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We propose a 2.1 μm high energy dissipative soliton resonant (DSR) fiber laser system based on mode-locked seed laser and dual-stage amplifiers. In the seed laser, nonlinear amplifying loop mirror technique is employed to realize mode-locking. The utilization of in-band pump scheme and long gain fiber enables effective exciting 2.1 μm pulses. A section of ultra-high numerical aperture fiber (UHNAF) with normal dispersion and high nonlinearity and an output coupler with large coupling ratio are used to achieve high energy DSR. By optimizing the UHNAF length to 55 m, a 2103.7 nm, 88.1 nJ DSR with a 3-dB spectral bandwidth of 0.48 nm and a pulse width of 17.1 ns is obtained under proper intracavity polarization state and pump power. The output power and conversion efficiency are 0.233 W and 4.57 %, respectively, both an order of magnitude higher than previously reported holmium-doped DSR seed lasers. Thanks to the high output power and

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“…Laser sources generating 2 µm radiation have many industrial and scientific applications such as absorption spectroscopy, chemical analysis [1,2], biomedicine [3,4] and security, because of presence of lines in absorption spectra of many important molecules (water, carbon dioxide, and so on) in this spectral range. These radiation sources are well suited for remote ecology sensing, weather analysis as well as for monitoring the greenhouse effect [5].…”

Section: Introductionmentioning

confidence: 99%

“…These radiation sources are well suited for remote ecology sensing, weather analysis as well as for monitoring the greenhouse effect [5]. In particular, the third most important long-lived greenhouse gas-nitric oxide (N 2 O)plays an important role in the process of stratospheric ozone depletion and has spectral lines around 2.1 µm [3]. The sources can be also used in biomedicine for a 13 C-urease breath test for non-invasive diagnostics of Helicobacter pylori bacterium.…”

Section: Introductionmentioning

confidence: 99%

Continuous-wave single-frequency holmium-doped fiber laser based on saturable absorber

Vladimirskaya¹,

Kamynin²,

Lobach³

et al. 2023

Laser Phys.

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The single-frequency holmium-doped fiber laser with spectral filtration based on a dynamics filter formed in heavily holmium-doped fiber is presented. The continuous wave lasing on a single longitudinal mode in the spectral region of 2.1 μm with an output power of 1.6 mW is shown when the filtering fiber is cooled with liquid nitrogen. The optical signal-to-noise ratio and the relative intensity noise were more than 30 dB and — 62.8 dB Hz−1, respectively. The developed source can be used for gas spectroscopy as well as for remote atmosphere sensing of such gases as carbon dioxide, nitric oxide, ammonia, carbon monoxide and others.

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Ex Vivo Exposure to Soft Biological Tissues by the 2-μm All-Fiber Ultrafast Holmium Laser System

Cited by 14 publications

References 53 publications

Effect of Laser-Induced Optical Breakdown on the Structure of Bsa Molecules in Aqueous Solutions: An Optical Study

Effect of Laser-Induced Optical Breakdown on the Structure of Bsa Molecules in Aqueous Solutions: An Optical Study

2.1 m, high-energy dissipative soliton resonance from a holmium-doped fiber laser system

Continuous-wave single-frequency holmium-doped fiber laser based on saturable absorber

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