Examination of glucoraphanin content in broccoli seedlings over growth and the impact of hormones and sulfur-containing compounds

Kim, Young Cheon; Hussain, Muhammad; Anarjan, Mahdi Badri; Lee, Sanghyeob

doi:10.1007/s11816-020-00617-6

Cited by 7 publications

(2 citation statements)

References 31 publications

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“…LSU genes are widely conserved throughout angiosperms and they are activated in response to sulfur deficiency in various species [27], indicating that they have an important role in regulating sulfur metabolism across different species. Studies have demonstrated that in broccoli, similar to in Arabidopsis, sulfur supply has a great impact on GSLs content [28,29]. The proper application of sulfur fertilizer is beneficial to increase GSLs, while inadequate supply of sulfur not only restricts GSLs biosynthesis but also lead to GSLs degradation [30,31].…”

Section: Discussionmentioning

confidence: 99%

Overexpression of BoLSU1 and BoLSU2 Confers Tolerance to Sulfur Deficiency in Arabidopsis by Manipulating Glucosinolate Metabolism

Yang,

Zhou,

Zhang

et al. 2023

IJMS

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Sulfur is an essential element for plant growth, development and resistance to environmental stresses. Glucosinolates (GSLs), a group of sulfur rich secondary metabolites found in Brassicaceae plants, are known for their defensive properties against pathogens and herbivores. Due to their integration of a large proportion of total sulfur, their biosynthesis and degradation are closely linked to sulfur metabolism. It has been demonstrated that GSLs can be broken down to release sulfur and facilitate the production of other thio-metabolites when the plant is under stress. However, the regulation of this process is still not fully understood. In this study, we constructed two broccoli LSU (low sulfur responsive) gene overexpressing lines, 35S::BoLSU1 and 35S::BoLSU2, to detect changes in GSL metabolism after sulfur deficiency treatment. The results showed that BoLSU1 and BoLSU2 inhibit the biosynthesis of aliphatic GSLs, while also promoting their degradation and increasing the content of glutathione (GSH), leading to the reallocation of sulfur from the GSL pool to other thio-metabolites such as GSH. Furthermore, this regulation of GSL metabolism mediated by BoLSU1 and BoLSU2 is found to be dependent on myrosinases BGLU28 and BGLU30. Our study provides insight into the physiological role of LSU proteins and their regulation of sulfur metabolism.

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Section: Discussionmentioning

confidence: 99%

Overexpression of BoLSU1 and BoLSU2 Confers Tolerance to Sulfur Deficiency in Arabidopsis by Manipulating Glucosinolate Metabolism

Yang,

Zhou,

Zhang

et al. 2023

IJMS

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“…Brassica crops have high N requirements because their seeds synthesize large amounts of TP [26]. Brassicas also have a high demand for S, which directly participates in the biosynthesis of sulfur-containing amino acids (cystine, cysteine, methionine) that act as precursors of biologically active compounds such as alkene GSLs, glutathione, thiamine (vitamin B1), biotin (vitamin H), coenzyme A, lipoic acid, thioredoxin, and sulfolipids [22,[27][28][29][30]. Nitrogen and S fertilization significantly affect the yield and quality of seeds in Brassica oilseed crops, including winter rapeseed [31], camelina [32][33][34], Indian mustard, spring rapeseed and field mustard-type canolas, and Indian mustard and white mustard-type mustards [35].…”

Section: Introductionmentioning

confidence: 99%

Oilseed Radish: Nitrogen and Sulfur Management Strategies for Seed Yield and Quality—A Case Study in Poland

Szatkowski,

Antoszkiewicz,

Purwin

et al. 2024

Agriculture

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Nitrogen (N) and sulfur (S) fertilization significantly affect seed yield and quality in Brassica oilseed crops. The effect of N and S management on the crop parameters (plant height, stem-base diameter, and number of branches), yield (seed yield components, seed and straw yields, harvest index—HI), and the quality of the seeds and oil (crude fat—CF, total protein—TP, crude fiber—CFR, fatty acids profile—FA, acid detergent fiber; and neutral detergent fiber) of oilseed radish (Raphanus sativus L. var. oleiformis Pers.) was analyzed in the study. The effect of N and S fertilization was evaluated in a field experiment in Bałcyny (north-eastern Poland) in 2020–2022. The experiment had a split-plot design with two factors and three replications. The first factor was the N rate (0, 30, 60, 90, 120 kg ha−1) and the second factor was the S rate (0, 15, 30 kg ha−1). Nitrogen fertilization stimulated stem elongation and branching. The average oilseed radish (OSR) seed yield ranged from 0.59 to 1.15–1.25 Mg ha−1. Seed yields increased significantly, up to 90 kg N ha−1 and 15 kg S ha−1. The N fertilizer use efficiency (NFUE) of OSR decreased with a rise in the N rate (from 4.22 to 2.19 kg of seeds per 1 kg N). The application of S did not increase NFUE. The HI ranged from 10% (0–30 kg N ha−1) to 12% (60 kg N ha−1). The contents of CF, TP, and CFR in OSR seeds (kg−1 dry matter—DM) were 383–384 g, 244–249 g, and 97–103 g, respectively. Nitrogen fertilization decreased the CF content (by 5%) and increased the contents of TP (by 5%) and CFR (by 16%) in OSR seeds. Sulfur fertilizer applied at 30 kg ha−1 decreased the CF content (by 2%), but it did not alter the content of TP or CFR. Oilseed radish oil contained 68–70% of monounsaturated FAs (MUFAs) (erucic acid accounted for 2/3 of the total MUFAs), 24–25% of polyunsaturated FAs (PUFAs), and 6–8% of saturated FAs (SFAs). Nitrogen fertilization increased the proportions of SFAs and PUFAs in OSR oil. Nitrogen rates of 60–90 kg ha−1 increased the contents of alpha-tocopherol (α-T), beta-tocopherol (β-T), and gamma-tocopherol (γ-T) in OSR seeds by 32%, 40%, and 27%, respectively. Sulfur fertilization increased the content of PUFAs and decreased the content of MUFAs in OSR oil, while it increased the contents of α-T (by 15%) and γ-T (by 19%) in OSR seeds. Proper N and S management in OSR cultivation can improve crop productivity and the processing suitability of seeds.

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Development of glucoraphanin-rich broccoli (Brassica oleracea var. italica) by CRISPR/Cas9-mediated DNA-free BolMYB28 editing

Kim

Ahn

Cha

et al. 2022

Plant Biotechnol Rep

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Examination of glucoraphanin content in broccoli seedlings over growth and the impact of hormones and sulfur-containing compounds

Cited by 7 publications

References 31 publications

Overexpression of BoLSU1 and BoLSU2 Confers Tolerance to Sulfur Deficiency in Arabidopsis by Manipulating Glucosinolate Metabolism

Overexpression of BoLSU1 and BoLSU2 Confers Tolerance to Sulfur Deficiency in Arabidopsis by Manipulating Glucosinolate Metabolism

Oilseed Radish: Nitrogen and Sulfur Management Strategies for Seed Yield and Quality—A Case Study in Poland

Development of glucoraphanin-rich broccoli (Brassica oleracea var. italica) by CRISPR/Cas9-mediated DNA-free BolMYB28 editing

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