Exercise-induced fibre type transitions with regard to myosin, parvalbumin, and sarcoplasmic reticulum in muscles of the rat

Green, H. J.; Klug, G. A.; Reichmann, Heinz; Seedorf, Udo; Wiehrer, Walter; Pette, Dirk

doi:10.1007/bf00587545

Cited by 168 publications

(90 citation statements)

References 40 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Muscle ®bre type transformations of the order Type 2b to Type 2a are known to occur with increased physical activity while transformation into Type 1 ®bres does not normally occur. 30 The present ®ndings are consistent with reduced levels of physical activity with age in the general population which may alter ®bre type composition towards a greater proportion of Type 2b ®bres ± ®bres which have a reduced capacity for fat utilisation.…”

Section: Discussionsupporting

confidence: 84%

Muscle fibre type composition in infant and adult populations and relationships with obesity

et al. 1997

View full text Add to dashboard Cite

OBJECTIVES: To investigate the ®bre type composition of skeletal muscle in infants and young children and to compare the ®ndings to an adult population. To relate the ®bre type pro®le of skeletal muscle in adults to measures of adiposity. DESIGN: Cross-sectional studies of skeletal muscle ®bre composition in infants and adults with measures of adiposity in the adults. SUBJECTS: 21 healthy infants and young children (age: 3±21 months) and 40 healthy adult Australian Caucasians (age: 26±62 y; BMI: 18±48 kg/m 2 ). MEASUREMENTS: Skeletal muscle ®bre type composition (by myosin ATPase method) and relative body fatness (BMI, waist circumference and waist/hip ratio (WHR)). RESULTS: Infants and young children had signi®cantly lesser proportions of glycolytic Type 2b ®bres (6.2 AE 1.1%; range 0.3±18.9%) compared with adults (20.5 AE 1.6%; range 4.9±36.0%) (p`0.0001). The percentage of Type 2b ®bres was directly related to BMI (r 0.44, p 0.02), waist circumference (r 0.49, p 0.009) and WHR (r 0.44, p 0.02) in adults. A signi®cant, direct relationship was also found between the proportion of glycolytic Type 2b ®bres and age in the adults (r 0.45, p 0.01). CONCLUSION: Skeletal muscle ®bre type composition is different in infants and adults and there is an age-dependent increase in Type 2b ®bres over the lifespan. An increased proportion of glycolytic Type 2b ®bres is associated with obesity in adults. Results support a gene-environment interaction on ®bre type composition in human skeletal muscle.

show abstract

Section: Discussionsupporting

confidence: 84%

Muscle fibre type composition in infant and adult populations and relationships with obesity

et al. 1997

View full text Add to dashboard Cite

show abstract

“…This parvalbumin͞actin ratio puts parvalbumin expression in these myocytes in the range of published parvalbumin expression levels for the rat fast twitch extensor digitorum longus (14). Immunofluorescence microscopy showed parvalbumin expression was detected at a similar level in Ϸ95% of myocytes with uniform expression throughout the cell (Fig.…”

Section: Resultsmentioning

confidence: 53%

Parvalbumin gene transfer corrects diastolic dysfunction in diseased cardiac myocytes

Wahr

Michele

Metzger

1999

Proc. Natl. Acad. Sci. U.S.A.

View full text Add to dashboard Cite

Heart failure frequently involves diastolic dysfunction that is characterized by a prolonged relaxation. This prolonged relaxation is typically the result of a decreased rate of intracellular Ca 2؉ sequestration. No effective treatment for this decreased Ca 2؉ sequestration rate currently exists. As an approach to possibly correct diastolic dysfunction, we hypothesized that expression of the Ca 2؉ binding protein parvalbumin in cardiac myocytes would lead to increased rates of Ca 2؉ sequestration and mechanical relaxation. Parvalbumin, which is normally absent in cardiac tissue, is known to act as a soluble relaxing factor in fast skeletal muscle fibers by acting as a delayed Ca 2؉ sink. As a test of the hypothesis, gene transfer was used to express parvalbumin in isolated adult cardiac myocytes. We report here that expression of parvalbumin dramatically increases the rate of Ca 2؉ sequestration and the relaxation rate in normal cardiac myocytes. Importantly, parvalbumin fully restored the relaxation rate in diseased cardiac myocytes isolated from an animal model of human diastolic dysfunction. These findings indicate that parvalbumin gene transfer offers unique potential as a possible direct treatment for diastolic dysfunction in failing hearts. H eart failure is a leading cause of hospitalization in the U.S., diagnosed in Ϸ700,000 new patients annually (1). About a third of all heart failure involves diastolic dysfunction (2), which frequently is the result of an inappropriate prolongation of the intracellular Ca 2ϩ transients that trigger cardiac muscle contraction (3-6). Consequently, the heart fails to relax appropriately between contractions, leading to an increased stiffness of the myocardium during diastole and thereby generating excessive resistance to filling. Currently there is no clinical treatment that directly ameliorates this prolonged Ca 2ϩ transient. Recent research into alleviating this form of diastolic dysfunction has centered primarily on increasing the rate of Ca 2ϩ uptake by the sarcoplasmic reticulum (SR), either through increased SR Ca 2ϩ pumping rates or increased expression of the SR pump. These are energy-dependent approaches, however, and energy production in failing hearts also is frequently impaired (5, 6). As a different approach, we used adenoviral-mediated gene transfer to express the soluble relaxing factor parvalbumin in cardiac myocytes. Parvalbumin, which is not naturally found in cardiac tissue, is a Ca 2ϩ binding protein whose Ca 2ϩ affinity is intermediate between the thin filament regulatory protein troponin C and the SR Ca 2ϩ pump. This intermediate Ca 2ϩ affinity allows parvalbumin to enhance the rate of relaxation in fast skeletal muscle by acting as a Ca 2ϩ sink to temporarily store Ca 2ϩ before SR uptake (7-9). Relaxation thus becomes dependent on the rate of Ca 2ϩ binding to parvalbumin, independent of energy status, rather than dependent on the slower, energy requiring SR Ca 2ϩ uptake (see Fig. 2 A, Inset). Parvalbumin is thought to be complexed to Mg 2ϩ at rest, ...

show abstract

“…This may have been a result of an elevation of the fatigue index due to fast to slow transition. Generally, fast-to-slow transition has been reported to be induced by electric stimulation at a relatively low frequency (6,14) and endurance exercise (11,12), but in our experiment it was induced by hypoventilation disorder. This alteration is thought to be caused by inaction of physical activity.…”

Section: Referencescontrasting

confidence: 55%

The effects of hypoventilation disorder on physiological and biochemical properties of the hindlimb muscles

Imagita

Ogaki²,

Fukuoka

et al. 2013

Biomed. Res.

View full text Add to dashboard Cite

The purpose of this study was to determine the physiological and biochemical properties of hindlimb muscles after hypoventilation (HPO) induced by bilateral phrenic nerve denervation. Male Wistar rats (10 weeks-old) underwent HPO by the phrenic nerve denervation at the cervical level or sham surgery. Analyses were performed 4, 8, and 12 weeks after the surgery. The myosin heavy chain (MHC) isoform profile and in vitro isometric contractile properties of the soleus (SOL) and extensor digitrum longus muscles (EDL) were analyzed. From the postoperative period, HPO induced characteristic changes in SpO 2 such as hypoventilation disorder. After 12 weeks, significant increases in MHC1 and significant decreases in MHC2a were observed in the MHC isoform composition in SOL. Moreover, significant increases in MHC2a and significant decreases in MHC2b were also observed in the MHC isoform composition in EDL muscles in the HPO compared with sham (SHM) group. In our study, the tidal volume after unilateral phrenic nerve denervation decreased by approximately 12%, and that after bilateral phrenic nerve denervation decreased by approximately 35%. We concluded that the reduction in behavioral activity levels in the HPO group may have resulted in changes of the peripheral skeletal muscles as a result of disuse atrophy.

show abstract

Exercise-induced fibre type transitions with regard to myosin, parvalbumin, and sarcoplasmic reticulum in muscles of the rat

Cited by 168 publications

References 40 publications

Muscle fibre type composition in infant and adult populations and relationships with obesity

Muscle fibre type composition in infant and adult populations and relationships with obesity

Parvalbumin gene transfer corrects diastolic dysfunction in diseased cardiac myocytes

The effects of hypoventilation disorder on physiological and biochemical properties of the hindlimb muscles

Contact Info

Product

Resources

About