Exo-pectinase production by Bacillus pumilus using different agricultural wastes and optimizing of medium components using response surface methodology

Abstract

In this research, the production of exo-pectinase by Bacillus pumilus using different agricultural wastes was studied. Agricultural wastes containing pectin such as wheat bran, sugar beet pulp, sunflower plate, orange peel, banana peel, apple pomace and grape pomace were tested as substrates, and activity of exo-pectinase was determined only in the mediums containing sugar beet pulp and wheat bran. Then, effects of parameters such as concentrations of solid substrate (wheat bran and sugar beet pulp) (A), ammonium sulphate (B) and yeast extract (C) on the production of exo-pectinase were investigated by response surface methodology. First, wheat bran was used as solid substrate, and it was determined that exo-pectinase activity increased when relatively low concentrations of ammonium sulphate (0.12-0.21% w/v) and yeast extract (0.12-0.3% w/v) and relatively high wheat bran (~5-6% w/v) were used. Then, exo-pectinase production was optimized by response surface methodology using sugar beet pulp as a solid substrate. In comparison to P values of the coefficients, values of not greater than 0.05 of A and B (2) showed that the effect of these process variables in exo-pectinase production was important and that changes done in these variables will alter the enzyme activity.

Decolorization of azo dye Everdirect Supra Red BWS in an aqueous solution by heat-activated persulfate: optimization using response surface methodology

“…R 2 gives the idea about the correlation between the experimental results and the predicted results. The closer the R 2 value is to unity, the better the correlation between the experimental and predicted values and the better the model predicts the response [48]. The R 2 value of 0.9948 indicates the accuracy of the model for Everdirect Supra Red BWS dye removal by heat-activated persulfate and that the experimental results were near the results that were predicted from the model.…”

Review on bacterial production of alkaline pectinase with special emphasis on Bacillus species

“…can be also achieved economically by utilizing different agro-waste as substrate. It is proved that orange peel waste can be used as a sole carbon source for pectinase production by various strains of Bacillus (Kapoor et al, 2000;Embaby et al, 2014;Tepe & Dursun, 2014;Kaur & Gupta, 2017). Wheat bran is another substrate, proven as a cheap and easily available source throughout the year for higher pectinase production and many researchers used wheat bran as an economical carbon source for pectinase production by Bacillus spp.…”

Application and optimization of solid-state fermentation process for enhancing polygalacturonase production by Penicillium expansum

“…Biological conversion has been considered as a better way to deal with the agricultural wastes. For instance, banana peels, wheat straw, wheat bran, sugarcane bagasse, rice straw and soybean hulls have been used as the substrates for enzyme production [2,20] . The natural solid substrate in SSF not only offers the nutrients but also acts as the anchorage for the microbial cells [21] .…”

“…In the industrial area, pectinase is a common term of enzyme groups providing three different catalytic activities (hydrolysis, de-esterification or trans-elimination) which catalyze various pectin and pectic substances [1,2] . Pectin, which present in vegetables and fruits in large amount, remains in the pulp during extraction and causes cloudiness in juices.…”