Exocytosis in isolated gastric glands induced by secretagogues and hyperosmolarity

Gibert, Anne J.; Hersey, S. J.

doi:10.1007/bf00204783

Cited by 10 publications

(7 citation statements)

References 15 publications

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“…A recent study of isolated chief cells by Gibert and Hersey (1982) lends substantial support to our hypothesis. They found that chief cells from laboratory rabbits normally had electron dense product, whereas when placed in hyperosmolar solution the cells ultrastructurally resembled chief cells that we have found in stenodermatines.…”

Section: Gastric Chief Cellssupporting

confidence: 83%

Results of the Alcoa Foundation Suriname expeditions. VIII. Comparative ultrastructure of gastric mucosae in four genera of bats (Mammalia: Chiroptera), with comments on gastric evolution.

Phillips¹,

Studholme²,

Forman³

1984

Annals of the Carnegie Museum

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Ultrastructure of gastric mucosa was studied comparatively in six species representing four microchiropteran genera, Pteronotus parnellii, Phyllostomus hastatus, P. elongatus, Carollia perspicillata, Artibeus lituratus, and A. concolor, which have different diets. Overall, the ultrastructural comparisons were shown to have systematic value as well as value for determination of evolutionary patterns at the cellular and tissue levels. Generic differences were found in numbers and types of entero-endocrine cells; a glucagonproducing A cell was the only type common to all four genera. Generally, entero-endocrine cell types could be correlated with both diet and systematics and the specific composition of cell types could be explained in terms of potential interactions with other cell types within individual species. Intergeneric ultrastructural differences also were found among chief cells and their secretory products and among mucous neck cells. The chief cell differences were related to diet and it was clear that stenodermatine chief cells are functionally divergent and, therefore, might be useful to an understanding of evolution of cell types. Interfamilial histochemical analyses of mucous neck cell product appeared to loosely match diet (insectivore-omnivore versus strict frugivore), whereas ultrastructural features of these cells were more nearly indicative of systematic relationships among

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Section: Gastric Chief Cellssupporting

confidence: 83%

Results of the Alcoa Foundation Suriname expeditions. VIII. Comparative ultrastructure of gastric mucosae in four genera of bats (Mammalia: Chiroptera), with comments on gastric evolution.

Phillips¹,

Studholme²,

Forman³

1984

Annals of the Carnegie Museum

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“…Many secretogogues for pepsinogen have been identified but there may well be differences between species. These include cholinergic and adrenergic neurotransmitter agents, cholecystokinin (CCK) octapeptide, caerulein, gastrin, secretin, vasoactive intestinal peptide (VIP), cyclic AMP and hyperosmolarity (Gilbert & Hersey, 1982). Calcium may also be required for secretion.…”

Section: P E P S I N O G E N S E C R E T I O Nmentioning

confidence: 99%

Nutritional Regulation of Gastric Secretion, Digestion and Emptying

Low¹

1990

Nutr. Res. Rev.

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“…At least two classes of secretagogues are recognized: those postulated to act through elevation of cellu- forskolin (F), 1 x lar cAMP levels, and substances that appear to be similar to CCK in their action, and may be mediated by Ca2+ (Kasbekar et al, 1983). In addition to these two classes of secretagogues, secretion can be elicited without drugs by placing the glands in hyperosmolar medium (Gibert and Hersey, 1982). The two classes of secretagogues and hyperosmolarity respond differently to ionic changes and transport inhibitors.…”

Section: Discussionmentioning

confidence: 99%

“…The incubation medium contained, in mM: NaCl, 132.4; KC1,5.4; Na2HP04, 5.0; NaH2P04, 1.0; MgS04, 1.2; CaC12, 1.0; pyruvate, 1.0; dithiothreitol, 0.5; phenol red, 10 mgAiter; glucose, 2 mg/ml; bovine plasma albumin 1 mg/ml (initial pH was 7.4). Osmolarity was increased by the addition of mannitol, bringing the final osmolarity of the medium to 450 mOsm (Gibert and Hersey, 1982).…”

Section: Gastric Gland Preparationmentioning

confidence: 99%

“…Exocytosis has been demonstrated with electron microscopy in chief cells of isolated gastric glands not only in response to secretagogues but also in response to hyperosmolar medium (Gibert and Hersey, 1982). Secretion in response to hyperosmolar medium is somewhat surprising, since in some systems, like chromaffin cells, hyperosmolarity inhibits exocytosis (Pollard et al, 1979).…”

mentioning

confidence: 99%

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Effect of ouabain and furosemide on pepsinogen secretion by gastric glands in vitro

Gibert

Hersey

1984

Journal Cellular Physiology

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Gastric glands were isolated from rabbit stomach and pepsinogen secretion was measured after stimulation with isoproterenol, forskolin, 8-bromo cyclic adenosine monophosphate (8-bromo cAMP), cholecystokinin octapeptide (CCK-OP), carbachol, and hyperosmolar medium. The responses to these stimuli in medium containing 143 mM Na+ and 5.4 mM K+ (normal medium) were compared with responses to the same stimuli in media containing either 0 Na+ and 5.4 mM K+, or 143 mM Na+ and O K+. In addition, the effects of ouabain and furosemide on secretion elicited by these stimuli were determined. Medium containing 0 Na+ inhibited all stimuli. Medium containing 0 K+ inhibited the action of 8-bromo cAMP and stimuli postulated to be mediated by cAMP. Ouabain inhibited the same stimuli as O K+ medium, and, in addition, inhibited the response to hyperosmolar medium. However, ouabain enhanced the response to CCK-OP. Furosemide inhibited the response to hyperosmolar medium but had no effect on the action of any secretagogue employed. Intraglandular [Na+] increased and [K+] decreased after exposure to K+-free medium or ouabain. cAMP content of the glands was assayed after stimulation with both isoproterenol and hyperosmolar medium. Isoproterenol and hyperosmolar medium significantly increased cAMP levels. The results are discussed in relation to possible involvement of ion transport or intracellular ion concentration in the secretory process.

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Exocytosis in isolated gastric glands induced by secretagogues and hyperosmolarity

Cited by 10 publications

References 15 publications

Results of the Alcoa Foundation Suriname expeditions. VIII. Comparative ultrastructure of gastric mucosae in four genera of bats (Mammalia: Chiroptera), with comments on gastric evolution.

Results of the Alcoa Foundation Suriname expeditions. VIII. Comparative ultrastructure of gastric mucosae in four genera of bats (Mammalia: Chiroptera), with comments on gastric evolution.

Nutritional Regulation of Gastric Secretion, Digestion and Emptying

Effect of ouabain and furosemide on pepsinogen secretion by gastric glands in vitro

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