Exogenous glutathione modulates redox homeostasis in okra (Abelmoschus esculentus) during storage

Li, Xu; Bao, Zeyang; Chen, Yana; Lan, Qingqing; Song, Chunbo; Shi, Lijun; Chen, Wei; Cao, Shifeng; Yang, Ziyi; Zheng, Qingbo

doi:10.1016/j.postharvbio.2022.112145

Cited by 22 publications

(7 citation statements)

References 33 publications

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“…Specifically, it was noticed that the generation of •OH continuously decreased in the gut with increasing Vc concentrations in the diet of PS-feeding superworms, which caused a gradual decline in PS oxidative depolymerization capacity in the superworm guts (Figure S7). Additionally, GSH, as another important ROS scavenger in organisms, 24,34 was confirmed to have a similar effect on PS biodegradation as Vc through scavenging of ROS generated in the gut (Figures 3 and 4 and Tables S1−S3). Overall, these results showed convincing evidence that the spontaneous generation of ROS triggered oxidative degradation of ingested PS, resulting in the rapid and effective biodegradation or mineralization of PS or PS in the gut of plastic-eating insect larvae.…”

Section: Ros Generation and Subsequent Effect On Oxidative Degradatio...mentioning

confidence: 78%

Reactive Oxygen Species Triggered Oxidative Degradation of Polystyrene in the Gut of Superworms (Zophobas atratus Larvae)

Chen

Zhang

Xing

et al. 2023

Environ. Sci. Technol.

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Oxidative decomposition of polystyrene (PS) by insects has been previously demonstrated, yet little is known about the oxidation mechanism and its effect on the metabolism of plastics within the insect gut. Here, we demonstrate the generation of reactive oxygen species (ROS) in the gut of superworms (Zophobas atratus larvae) under different feeding trails, which in turn induced the oxidative decomposition of ingested PS. The ROS were commonly generated in the larva gut, and PS consumption resulted in a significant increase of ROS with a maximum •OH of 51.2 μmol/kg, which was five times higher than in the bran feeding group. Importantly, scavenging of ROS significantly decreased the oxidative depolymerization of PS, indicating a vital role of ROS in effective PS degradation in the gut of superworms. Further investigation suggested that the oxidative depolymerization of PS was caused by the combinatorial effect of ROS and extracellular oxidases of gut microbes. These results demonstrate that ROS were extensively produced within the intestinal microenvironment of insect larvae, which greatly favored the digestion of ingested bio-refractory polymers. This work provides new insights into the underlying biochemical mechanisms of plastic degradation in the gut.

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Section: Ros Generation and Subsequent Effect On Oxidative Degradatio...mentioning

confidence: 78%

Reactive Oxygen Species Triggered Oxidative Degradation of Polystyrene in the Gut of Superworms (Zophobas atratus Larvae)

Chen

Zhang

Xing

et al. 2023

Environ. Sci. Technol.

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“…These results revealed that the elevated CO 2 concentration of 6.2–7.1% and the low O 2 concentration of 0.5–0.6% in the PAT bags might decrease the superoxide anion and hydroxyl radical [ 15 ], thereby delaying membrane damage and lipid peroxidation. It was also reported that the disrupted ROS metabolic balance resulted in damage to the cell membrane system and the postharvest senescence of vegetables [ 41 ].…”

Section: Resultsmentioning

confidence: 99%

Polypropylene/Poly(butylene adipate-co-terephthalate) Breathing Film for Inhibiting Pseudomonas and Maintaining Microbial Communities and Postharvest Quality of Allium mongolicum Regel during Storage

Bu,

Hu,

Han

et al. 2023

Foods

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Allium mongolicum Regel (A. mongolicum) is a healthy edible plant but highly perishable with a short shelf life of 1–2 d. Modified atmosphere packaging (MAP) could inhibit the postharvest senescence and decay of the vegetables. Thus, the aim of this study was to apply MAP with different gas permeabilities to the storage of A. mongolicum and evaluate its effects on maintaining microbial communities and the postharvest quality of A. mongolicum. The results showed that polypropylene/poly(butylene adipate-co-terephthalate) (PP/PBAT, abbreviated as PAT) MAP was suitable for the storage of A. mongolicum by establishing an optimal atmosphere of 0.5–0.6% O2 and 6.2–7.1% CO2 in the bag. It could delay the postharvest senescence of A. mongolicum and maintain its quality by slowing down its respiration rate and weight loss, reducing cell membrane permeability and lipid peroxidation, maintaining the cell wall, and reducing infection and the growth of microorganisms. However, A. mongolicum in HPT was more perishable than that in PAT during storage. Pseudomonas was found to be the main spoilage bacteria, and they could also be effectively inhibited by PAT-MAP. The next-generation sequencing results also showed the growth of Escherichia-Shigella, Clostridium sensu stricto 1, Streptococcus, Aureobasidium, Didymella, and Fusarium, responsible for A. mongolicum decay or human disease, was well inhibited by PAT-MAP. The results suggested that PAT-MAP could be used to maintain microbial diversity and the postharvest quality of A. mongolicum under cold storage conditions. It provided a feasible solution for the preservation, food quality, and safety control of A. mongolicum.

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“…It has been mentioned above that under 30 psu, BSO promoted an increase in the endogenous GSH content, DHAR activity and DHA content, indicating the complementary role of GSH and AsA in B. fuscopurpurea. Exogenous GSH could upregulate APX and AsA in higher plants (Yao et al, 2021;Li et al, 2022). The addition of exogenous GSH increased the AsA/DHA ratio, APX activity and expression of BfAPX gene in B. fuscopurpurea under hyposalinty, when the accumulation of endogenous GSH was weakened and the GSH/GSSG was lowered.…”

Section: 4mentioning

confidence: 94%

Ascorbate−glutathione cycle involving in response of Bangia fuscopurpurea (Bangiales, Rhodophyta) to hyposalinity

et al. 2023

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Bangia fuscopurpurea is a widespread intertidal seaweed that is commercially cultured in China. This seaweed is frequently exposed to hyposalinity stress, but little is known about the adaptation mechanisms. Ascorbate−glutathione (AsA−GSH) cycle plays important roles in many organisms under a variety of abiotic stress, including hyposaline stress. In this study, we investigated the response of key metabolites and enzymes involved in the AsA−GSH cycle of B. fuscopurpurea under hyposalinity, with the addition of exogenous GSH and Lbuthionine-sulfoximine (BSO). The quantification of BfAPX gene expression was assessed across varied treatment regimens. And the putative interaction proteins of BfAPX were screened by yeast two hybrid system. It was found that under hyposalinity (15 and/or 0 psu), the content of reduced glutathione (GSH), total glutathione (GSH+ oxidized glutathione, GSSG) and cysteine, the ratio of GSH/GSSG and ascorbic acid (AsA)/ dehydroascorbic acid (DHA), and the activity of ascorbic acid peroxidase (APX) and monodehydroascorbate reductase (MDHAR) was significantly up-regulated. The hyposality-promoted GSH/GSSG was weakened while the glutathione reductase (GR) activity was promoted by adding exogenous GSH and BSO. The hyposality-promoted AsA/DHA ratio was strengthened by exogenous GSH but weakened by BSO. The dehydroascorbate reductase (DHAR) activity had no significant changes either with or without exogenous GSH under all salinities, while DHAR activity together with DHA content was enhanced by BSO. The expression of APX gene markedly increased under hyposalinity+BSO treatment. Putative interacting proteins of APX, including glutamate dehydrogenase 1a and fructose diphosphate aldolase, were identified through screening. The results indicated that the AsA−GSH cycle was involved in response of B. fuscopurpurea to hyposalinity by means of increasing GSH/GSSG ratio (through promoting GSH biosynthesis pathway and GSH regeneration from GSSG by GR catalyzation) and AsA/DHA ratio (promoting AsA regeneration through MDHAR). These findings would contribute to improve the aquaculture of this promising economic species and unveil how intertidal seaweeds address the global climate challenges.

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Exogenous glutathione modulates redox homeostasis in okra (Abelmoschus esculentus) during storage

Cited by 22 publications

References 33 publications

Reactive Oxygen Species Triggered Oxidative Degradation of Polystyrene in the Gut of Superworms (Zophobas atratus Larvae)

Reactive Oxygen Species Triggered Oxidative Degradation of Polystyrene in the Gut of Superworms (Zophobas atratus Larvae)

Polypropylene/Poly(butylene adipate-co-terephthalate) Breathing Film for Inhibiting Pseudomonas and Maintaining Microbial Communities and Postharvest Quality of Allium mongolicum Regel during Storage

Ascorbate−glutathione cycle involving in response of Bangia fuscopurpurea (Bangiales, Rhodophyta) to hyposalinity

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