Exosome-carried microRNA-based signature as a cellular trigger for the evolution of chronic lymphocytic leukemia into Richter syndrome

Jurj, Ancuța; Pop, Laura; Petrushev, Bobe; Pașca, Sergiu; Dima, Delia; Frinc, Ioana; Deak, Dalma; Desmirean, Minodora; Trifa, Adrian P.; Fetică, Bogdan; Gafencu, Grigore; Selicean, Sonia; Moisoiu, Vlad; Micu, Wilhelm-Thomas; Berce, Cristian; Sacu, Alexandra; Moldovan, Alin; Coliţă, Andrei; Bumbea, Horia; Tănase, Alina; Dăscălescu, Angela; Zdrenghea, Dumitru; Știufiuc, Rareș; Leopold, Nicolae; Tetean, R.; Burzo, Emil; Tomuleasa, Ciprian; Berindan‐Neagoe, Ioana

doi:10.1080/10408363.2018.1499707

Cited by 31 publications

(26 citation statements)

References 115 publications

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“…The internalization of the exosomes has been proven to influence recipient cells mainly due to the composition of the exosomal cargo [29]. "Signals" are encoded in the components of the exosomal cargo, consisting of fragmented nuclear DNA [30][31][32], mitochondrial DNA [33], proteins, growth factors [34] miRNAs [24,35], and long non-coding RNAs [36,37], making exosomes very important players in cell-to-cell communication. The exact mechanisms of how exosomal cargo is selected and loaded are still under constant discussion [38].…”

Section: Cell-to-cell Communication By Extracellular Vesiclesmentioning

confidence: 99%

MicroRNAs from Liquid Biopsy Derived Extracellular Vesicles: Recent Advances in Detection and Characterization Methods

Drula

Ott

Berindan‐Neagoe

et al. 2020

Cancers

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Liquid biopsies have become a convenient tool in cancer diagnostics, real-time disease monitoring, and evaluation of residual disease. Yet, the information still encrypted in the variety of tumor-derived molecules identified in biofluids has proven difficult to decipher due to the technological limitations imposed by their biological nature. Such is the case of extracellular vesicle (EV) encapsulated ncRNAs, which have gained traction in recent years as biomarkers. Due to their resilience towards degrading factors they may act as suitable disease indicators. This review addresses the less described issues in this context. We present an overview of less investigated biofluids that can be used for EV isolation in addition to different isolation approaches to overcome the technical challenges these specimens harbor. Furthermore, we summarize the latest technological advances providing improvement to ncRNA detection and analysis. Thereby, this review summarizes the current state-of-the-art methodologies regarding EV and EV derived miRNA analysis and how they compare to current approaches.

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Section: Cell-to-cell Communication By Extracellular Vesiclesmentioning

confidence: 99%

MicroRNAs from Liquid Biopsy Derived Extracellular Vesicles: Recent Advances in Detection and Characterization Methods

Drula

Ott

Berindan‐Neagoe

et al. 2020

Cancers

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“…These miRNAs are always associated with CLL pathogenesis [67]. Moreover, exosomal miRNAs could be responsible for the evolution of CLL in Richter's syndrome, according to Jurj and colleagues, who provided the evidence that miR-19 was the most abundant component of patient exosomes [69]. miR-19 up-regulated Ki67 and down-regulated p53, leading to apoptosis escape and CLL evolution in Richter's syndrome [69].…”

Section: A Focus On Hematological Malignanciesmentioning

confidence: 99%

Uncovering the Exosomes Diversity: A Window of Opportunity for Tumor Progression Monitoring

et al. 2020

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Cells can communicate through special “messages in the bottle”, which are recorded in the bloodstream inside vesicles, namely exosomes. The exosomes are nanovesicles of 30–100 nm in diameter that carry functionally active biological material, such as proteins, messanger RNA (mRNAs), and micro RNA (miRNAs). Therefore, they are able to transfer specific signals from a parental cell of origin to the surrounding cells in the microenvironment and to distant organs through the circulatory and lymphatic stream. More and more interest is rising for the pathological role of exosomes produced by cancer cells and for their potential use in tumor monitoring and patient follow up. In particular, the exosomes could be an appropriate index of proliferation and cancer cell communication for monitoring the minimal residual disease, which cannot be easily detectable by common diagnostic and monitoring techniques. The lack of unequivocal markers for tumor-derived exosomes calls for new strategies for exosomes profile characterization aimed at the adoption of exosomes as an official tumor biomarker for tumor progression monitoring.

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“…One of the most upregulated miRNAs in plasma-derived exosomes from CLL patients is miR-19b. This miRNA enhances the proliferation and even the invasiveness of CLL cells by upregulating Ki67 and downregulating TP53, leading to the evolution of therapy resistant CLL into Richter syndrome [100].…”

Section: Tumor Microenvironmentmentioning

confidence: 99%

Non-Coding RNAs as Cancer Hallmarks in Chronic Lymphocytic Leukemia

Fabris

Juráček

Calin

2020

IJMS

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The discovery of non-coding RNAs (ncRNAs) and their role in tumor onset and progression has revolutionized the way scientists and clinicians study cancers. This discovery opened new layers of complexity in understanding the fine-tuned regulation of cellular processes leading to cancer. NcRNAs represent a heterogeneous group of transcripts, ranging from a few base pairs to several kilobases, that are able to regulate gene networks and intracellular pathways by interacting with DNA, transcripts or proteins. Deregulation of ncRNAs impinge on several cellular responses and can play a major role in each single hallmark of cancer. This review will focus on the most important short and long non-coding RNAs in chronic lymphocytic leukemia (CLL), highlighting their implications as potential biomarkers and therapeutic targets as they relate to the well-established hallmarks of cancer. The key molecular events in the onset of CLL will be contextualized, taking into account the role of the “dark matter” of the genome.

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Exosome-carried microRNA-based signature as a cellular trigger for the evolution of chronic lymphocytic leukemia into Richter syndrome

Cited by 31 publications

References 115 publications

MicroRNAs from Liquid Biopsy Derived Extracellular Vesicles: Recent Advances in Detection and Characterization Methods

MicroRNAs from Liquid Biopsy Derived Extracellular Vesicles: Recent Advances in Detection and Characterization Methods

Uncovering the Exosomes Diversity: A Window of Opportunity for Tumor Progression Monitoring

Non-Coding RNAs as Cancer Hallmarks in Chronic Lymphocytic Leukemia

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