Experience with NESTROFT for screening for thalassemia trait/ minor: evaluation against CBC and HPLC in a high prevalence region in Saurashtra, Gujarat, India

Sorathiya, Varsha P.; Vachhani, Nishith A.; Nandani, Sanjeev L.; Vekariya, Daya J.; Kashiyani, Heena N.; Colah, Roshan

doi:10.18203/2320-6012.ijrms20200789

Cited by 4 publications

(4 citation statements)

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“…11 Red cell indices such as MCV (mean corpuscular volume), MCH (Mean Corpuscular Haemoglobin) and MCHC (mean corpuscular hemoglobin concentration) have a significant role in suspicion of beta-thalassemia. Low MCV, MCH and MCHC values were observed in cases of betathalassemia trait in the present study which correlated with Sorathiya et al 12 In a case of microcytic hypochromic anemia, RDW is less than 14.5% in iron deficiency and 14.5% in beta thalassemia trait. The RDW value of less than 14.5% was observed in 7 cases of beta thalassemia trait in the present study.…”

Section: Discussionsupporting

confidence: 90%

A clinico-haematological study of hereditary hemoglobinopathies: a tertiary care centre experience

Atla¹,

Botta²,

Balakrishnan³

et al. 2021

Int J Res Med Sci

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Background: Hemoglobinopathies are the cause of concern in India for not only its effect on the quality of life in patients but also for their inheritance patterns. Tribal population of Visakhapatnam district has a high chance of inheriting hemoglobinopathies due to their culture of consanguineous marriage. Aim and objectives of current study were to know the distribution of various abnormal haemoglobins in cases with clinical suspicion of hemoglobinopathies.Methods: This hospital-based observational study was conducted for a period of 10 months in the department of pathology, Andhra Medical College, Visakhapatnam. A total of 151 cases with suspected hemoglobinopathies, their parents, and siblings were screened for the presence of hemoglobinopathies. 3ml of venous blood was collected to perform complete blood count, peripheral smear, reticulocyte count, sickling test and High Performance liquid chromatography (HPLC).Results: In the present study, out of 151 cases, 55 cases (36.42%) were adults, and 96 cases (63.57%) cases were children. 67cases (44.37%) were asymptomatic and 84 (55.62%) were symptomatic. The most common symptom of subjects are fever (23 cases, 27.38%) and dyspnoea (22 cases, 26.19%). 85 cases (56.29%) had normal HPLC, and 66 cases (43.70%) had abnormal hemoglobin variants. The most common hemoglobinopathy detected by HPLC was sickle cell trait (36 cases, 23.84%) followed by homozygous sickle cell anemia 15 (9.93%). Other hemoglobinopathies detected were beta-thalassemia trait; 8 cases (5.29%) and compound heterozygous sickle beta-thalassemia 3 cases (1.98%).Conclusions: Endemic areas for hemoglobinopathies has to be screened with HPLC along with complete hemogram in suspicious cases for the better diagnosis and management of the condition.

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Section: Discussionsupporting

confidence: 90%

A clinico-haematological study of hereditary hemoglobinopathies: a tertiary care centre experience

Atla¹,

Botta²,

Balakrishnan³

et al. 2021

Int J Res Med Sci

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“…Semua study meneliti tentang fragilitas osmotik tabung tunggal, hematologi lengkap dan analisis Hb dan memperlihatkan sensitivitas fragilitas osmotik tabung tunggal yang bervariasi, berkisar antara 78,4 -100% dan spesifisitas antara 63,38-100%. 24 Terdapat perbedaan populasi target pada laporan penelitian tersebut. Kattamis et al, yang pertama menemukan tes fragilitas osmotik tabung tunggal untuk skrining talasemia beta carrier dengan berbagai konsentrasi larutan salin yang berbeda dan menemukan bahwa konsentrasi 0,36% merupakan konsentrasi terbaik mendeteksi talasemia minor.…”

Section: Pembahasanunclassified

“…Dari 1000 subjek penelitian yang dilakukan skrining, 78 individu terdiagnosis talasemia β trait (7,8%) menggunakan HPLC sebagai baku emas dengan kadar HbA2 ≥4.0%. 24 Sedikit berbeda dengan penelitian ini yang menemukan 27 dari 230 subjek (11,7%), menggunakan HPLC sebagai baku emas dengan kadar HbA2 >3,2%. Perbedaan ini dapat disebabkan karena perbedaan nilai cut-off kadar HbA2 yang digunakan.…”

Section: Pembahasanunclassified

“…Masing masing variabel penelitian dihitung terpisah, bukan berupa panel. 24 Terlihat bahwa sensitivitas dan spesifisitas tes fragilitas osmotik dan MCV, MCH tinggi. Hal yang berbeda dengan penelitian ini bahwa hanya tes fragilitas osmotik pada nilai MCV <80 fl yang menunjukkan sensitivitas yang cukup baik yaitu 70,6% tetapi mempunyai spesifisitas yang sangat rendah yaitu 20%.…”

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Uji Validitas Panel Pemeriksaan Mean Corpuscular Volume, Indeks Mentzer dan Fragilitas Osmotik Tabung Tunggal untuk Skrining Talasemia Minor terhadap Pemeriksaan HbA2 Menggunakan High Performance Liquid Chromatography

Fridayenti¹,

Dalimoenthe

Prihatni

et al. 2022

JIK

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Thalassemia is a disease caused by reduced or loss of synthesis of globin chains and then hemoglobin becomes abnormal. Characteristic screening in the population needs to be done to prevent marriage among people with thalassemia minor and reduce the birth of babies with thalassemia mayor. Combining several simple tests such as MCV, Mentzer index and single tube osmotic fragility test can increase the effectiveness of the test for screening of thalassemia minor. The purpose of this study was to determine the sensitivity and specificity of each panel for MCV-Mentzer index, MCV-single tube osmotic fragility, and Mentzer index- single tube osmotic fragility test panel against HbA2 in screening for the thalassemia minor. This study is observational with cross-sectional study design. The study was conducted at the Clinical Pathology Laboratory of Arifin Achmad Hospital Pekanbaru in June-August 2020. There were 27 subjects who had MCV values <80 fl out of 230 screened subjects. Then on 27 research subjects were calculated for Mentzer index, single tube osmotic fragility test and HPLC. The research results show the sensitivity, specificity, positive predictive value and negative predictive value of the MCV-Mentzer index panel for screening of thalassemia minor were 11.8%, 90%, 66.7% and 37.5%, respectively. For the MCV-single tube osmotic fragility panel was, respectively, 70.6%, 20%, 60% and 28.6% and for the Mentzer index- single tube osmotic fragility panel was 11.8%, 90%, 66, 7% and 37.5% respectively. The conclusion is that the MCV-single tube osmotic fragility panel has better sensitivity than the MCV-Mentzer index panel or the Menzer index-single tube osmotic fragility panel that it can be used to screen for thalassemia minor in the premarital population in Pekanbaru.

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Spectrum of β-Thalassemia and Other Hemoglobinopathies in the Saurashtra Region of Gujarat, India: Analysis of a Large Population Screening Program

Vachhani¹,

Vekariya²,

Colah

et al. 2022

Hemoglobin

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Experience with NESTROFT for screening for thalassemia trait/ minor: evaluation against CBC and HPLC in a high prevalence region in Saurashtra, Gujarat, India

Cited by 4 publications

References 8 publications

A clinico-haematological study of hereditary hemoglobinopathies: a tertiary care centre experience

A clinico-haematological study of hereditary hemoglobinopathies: a tertiary care centre experience

Uji Validitas Panel Pemeriksaan Mean Corpuscular Volume, Indeks Mentzer dan Fragilitas Osmotik Tabung Tunggal untuk Skrining Talasemia Minor terhadap Pemeriksaan HbA2 Menggunakan High Performance Liquid Chromatography

Spectrum of β-Thalassemia and Other Hemoglobinopathies in the Saurashtra Region of Gujarat, India: Analysis of a Large Population Screening Program

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