Experiences with an identification and quantification program for inhibitor-positive milk samples

Kress, Claudia; Seidler, C.; Kerp, Bianca; Schneider, Elisabeth; Usleber, Ewald

doi:10.1016/j.aca.2006.10.013

Cited by 12 publications

(8 citation statements)

References 9 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…Also, in Germany, in 2007, on another survey conducted by Kress and several co-authors during a program for identification of positive samples of milk 1.6% of their cases showed a presence of sulphonamide residues [24] .…”

Section: Khaskheli Et Al Argue That Inmentioning

confidence: 99%

Abuse of Pharmaceutical Drugs-antibiotics in Dairy Cattle in Kosovo and Detection of their Residues in Milk

Sulejmani¹,

Shehi²,

Hajrulai³

2012

J Ecosyst Ecogr

View full text Add to dashboard Cite

Introduction: Pharmaceutical drugs-antibiotics are given to livestock by veterinarians for the prevention or/and treatment of disease, but also as a stimulant for animal growth and conversion. Regardless of the way the drugs are inserted into the body of the animal, there will always be residual risk in milk which exceed the maximum level allowed. Thus, given that milk contains high level of drug residue, it can lead to health disorders in humans and in some cases even death. Aim of study: The main aim of this study is to discover the use of pharmaceutical drugs-antibiotics in dairy cows and to detect betalactam and sulfonamide residues in milk. Methods and materials used: In 2011, during the period January to August, 80% of dairy farms in the Republic of Kosovo were visited and 127 milk samples were collected from cows treated with pharmaceutical drugs-antibiotics. Samples were analysed ÿ'3fÿ'3fin the laboratories of the Veterinary Institute in Skopje using "Elissa" screening method and "HPLC" confirmatory method. Results: Of 127 samples of milk, 64 of them were contaminated with betalactam residues, while 24 with sulphonamide residues. Of them, the majority, 15 being beta-lactams and 4 sulphonamides. The study shows that over 70% of the total quantity of milk samples analysed contains residues of drugs, while over 20% of them have passed the maximum residue limit allowed. Conclusion: 70% of the drugs given to dairy cattle in Kosovo are pharmaceutical-antibiotics. Their residues are present in milk at levels high enough to cause severe damage to the entire public health. We conclude that pharmaceutical drugs-antibiotics that are used in humans should not be used in animals too and their provision should be limited to a reasonable and lawful level.

show abstract

Section: Khaskheli Et Al Argue That Inmentioning

confidence: 99%

Abuse of Pharmaceutical Drugs-antibiotics in Dairy Cattle in Kosovo and Detection of their Residues in Milk

Sulejmani¹,

Shehi²,

Hajrulai³

2012

J Ecosyst Ecogr

View full text Add to dashboard Cite

show abstract

“…As given in the year report of 2005 of Comité du Lait (Walloon region) the percentage of penalizations due to β-lactams was 94.5% in 2003, 90.0% in 2004, and 83.9% in 2005 (Comité du Lait 2006). In Germany, β-lactams could, in 95% of cases, be identified in inhibitor-positive milk samples (Kress et al 2007). Penicillin G was still the predominant antibiotic detected (74.6%) during regulatory control, followed by ceftiofur (11%), ampicillin/amoxicillin (6.3%) and isoxazolyl penicillins (3.2%).…”

Section: Introductionmentioning

confidence: 99%

Validation of the βeta-s.t.a.r. 1 + 1 for rapid screening of residues of β-lactam antibiotics in milk

Reybroeck¹,

Ooghe²,

Brabander³

et al. 2010

Food Additives & Contaminants: Part A

View full text Add to dashboard Cite

The 2-min protocol (1 + 1) for the βeta-s.t.a.r. (manufactured by Neogen Corporation, Lansing, MI, USA) was validated at the Technology and Food Science Unit of the Institute for Agricultural and Fisheries Research according to Commission Decision 2002/657/EC. The test was very selective for the group of β-lactam compounds: the only interference found was by clavulanic acid at 2500 μg kg−1 and above. The modified protocol (βeta-s.t.a.r. 1 + 1) detected all β-lactams with a maximum residue limit (MRL) in milk, but not all these compounds were detected at their respective MRL. The detection of cefalexin (detection capability = 6000 μg kg−1; MRL = 100 μg kg−1) and penethamate (detection capability = 80 μg kg−1; MRL = 4 μg kg−1) was especially poor, and also ceftiofur was only detected from 500 μg kg−1 (MRL = 100 μg kg−1). The repeatability of the reader and of the test was very good. The test was very robust: test results were not significantly influenced by small changes in the test protocol, by the milk composition or by the type of milk. The test was also suitable to test the milk of animal species other than cow. Favourable results were obtained in testing monitoring samples, in two national ring trials, and in an international proficiency test. The βeta-s.t.a.r. 1 + 1 is a very fast, simple, and reliable test that could be used at the farm level to prevent tanker milk contamination by β-lactams.

show abstract

“…Antibiotics have been identified in other parts of the world falling above the standard residual limits, including Germany (Kress et al, 2007), Netherlands (Abjean et al 2000), Mexico (Tolentino et al, 2005) Turkey (Alkan, 2007) among others. Other African countries have also been reported to have milk contaminated with Antibiotic residues.…”

Section: Introductionmentioning

confidence: 99%

Assessment of sulphonamides and tetracyclines antibiotic residue contaminants in rural and peri urban dairy value chains in Kenya

et al. 2017

View full text Add to dashboard Cite

Background: Antibiotic residues are drug substances found in food from plants or animals initially exposed to antibiotics. In animal husbandry antibiotics have widely been used for the treatment of animal diseases. These residues have the ability to expose the public to serious health hazards. In Kenya drug residues have not only been related to lack of withdrawal periods but also to intentional addition to extend milk's shelf life. Results: The aim of this study was to investigate the occurrence of 13 veterinary drugs of tetracyclines and sulphonamides along the dairy sub value chain. The study was carried out in Nakuru County which is the leading milk producer in the country. A total of 229 samples were analysed from rural and 80 samples from peri-urban. These were collected from different nodes of the value chain; the farm, milk transporters and at the bulking centers between January 2014 and November 2015. Screening of samples was done by Charm II Blue -Yellow-test while confirmation was done by HPLC-UV for sulfachloropyradizine (SCL), sulfadiazine (SDZ), sulfadimidine (SMTZ), sulfaquinoxaline (SQ), sulfamerazine (SMR), sulfathiazole (STZ), sulfamethoxazole (SMX), sulfadoxin (SDOX), sulfadimethoxin (SDM), oxytetracycline (OTC), doxycycline hyclate (DC), chlortetracycline hydrochloride (CTC) and tetracycline hydrochloride (TC). In the rural 72 out of 229 (31.4%) samples were positive after screening while none of the samples confirmed the presence of tetracyclines after analysis with HPLC-UV. Sulphonamides confirmed after analysis with HPLC-UV were all above the EU MRL limits. In the peri urban 28.8% (23/80) of the samples were positive for antibiotic residues. Tetracyclines were not detected in confirmation while 60% of the positive samples were positive for sulphonamides out of which 71% were above the regulatory limits. Highest percentage of antibiotics was detected in rural farms (46.7%) and at peri urban bulking centers (50%). Conclusion: The study concluded that antibiotic residues along the dairy value chain are majorly from the farm due to lack of withdrawal periods followed by intentional addition along the value chain. Value chain actors should also be trained on ways of avoiding antibiotic residues from entering the dairy value chain to protect the public from health effects related to antibiotic residues.

show abstract

Experiences with an identification and quantification program for inhibitor-positive milk samples

Cited by 12 publications

References 9 publications

Abuse of Pharmaceutical Drugs-antibiotics in Dairy Cattle in Kosovo and Detection of their Residues in Milk

Abuse of Pharmaceutical Drugs-antibiotics in Dairy Cattle in Kosovo and Detection of their Residues in Milk

Validation of the βeta-s.t.a.r. 1 + 1 for rapid screening of residues of β-lactam antibiotics in milk

Assessment of sulphonamides and tetracyclines antibiotic residue contaminants in rural and peri urban dairy value chains in Kenya

Contact Info

Product

Resources

About