Experimental infection and the state of viral latency of adult Tupaia with herpes simplex virus type 1 and 2 and infection of Juvenile Tupaia with temperature-sensitive mutants of HSV type 2

Abstract

The susceptibility of adult Tupaia Belangeri to infection with herpes simplex virus (HVS) was investigated. Adult animals were inoculated intraperitoneally with HSV type 1 or 2. With the exception of HSV-2, strain HG-52, 10(5)--10(6) PFU of all HSV strains caused lethal infection irrespective of the age of the animals. Infections HSV was recovered from the spinal cord of those animals which had survived infection with a low dose of virus. The DNA of the recovered viruses was compared to the DNA of the inoculated HSV. The viral genome of the recovered HSV was unchanged as judged by analysis of the fragment pattern of the viral DNA's using restriction endonucleases. Animals which had survived the first HSV infection were protected against a second infection even at highly lethal doses of HSV-1 or 2. Juvenile Tupaia survived infection with temperature-sensitive mutants of HSV-2, strain HG-52, which induced protection against a second infection with lethal doses of HSV-1 or 2.

Basic research on herpes simplex viruses: are mutants still needed?

“…0.62–0.72 [ 40 ]/used in research project: Complementation analyses [ 5 ]; Enzymes in herpesvirus-infected cells [ 41 ]; Virus pathogenicity and latency in prosimians [ 42 ]. ts2 / DNA − //Isolated by M. Timbury, Glasgow/ used in research project: Complementation analyses [ 5 ]; Enzymes in herpesvirus-infected cells [ 41 ]; Virus pathogenicity and latency in prosimians [ 42 ]. ts5 / DNA + /Isolated by M. Timbury, Glasgow/ts lesion ca.…”

“…ts5 / DNA + /Isolated by M. Timbury, Glasgow/ts lesion ca. 0.26–0.38 [ 40 ]/used in research project: Complementation analyses [ 5 ]; Enzymes in herpesvirus-infected cells [ 41 ]; Virus pathogenicity and latency in prosimians [ 42 ]. ts6 / DNA − /isolated by M. Timbury, Glasgow/ts lesion ca.…”

“…ts9 / DNA − /Isolated by M. Timbury, Glasgow/ts lesion ca. 0.44–0.53 [ 40 ]; /used in research project: Complementation analyses [ 5 ]; Enzymes in herpesvirus-infected cells [ 41 ]; Virus pathogenicity and latency in prosimians [ 42 ]. ts10 / DNA − / Isolated by M. Timbury, Glasgow/used in research project: Enzymes in herpesvirus-infected cells [ 41 ]; Virus pathogenicity and latency in prosimians [ 42 ].…”

“…0.44–0.53 [ 40 ]; /used in research project: Complementation analyses [ 5 ]; Enzymes in herpesvirus-infected cells [ 41 ]; Virus pathogenicity and latency in prosimians [ 42 ]. ts10 / DNA − / Isolated by M. Timbury, Glasgow/used in research project: Enzymes in herpesvirus-infected cells [ 41 ]; Virus pathogenicity and latency in prosimians [ 42 ]. ts12 / DNA ± /Isolated by M. Timbury, Glasgow/used in research project: Complementation analyses [ 5 ]; Enzymes in herpesvirus-infected cells [ 41 ]; Virus pathogenicity and latency in prosimians [ 42 ].…”

Cloning of full-length coding sequence of tree shrew CD4 and prediction of its molecular characteristics

“…树鼩（tree shrews, Tupaia belangeri）是外形酷 似松鼠的小型哺乳动物 (Cao, 2003)，生活在东南亚 热带雨林地区，在我国云南、广西和海南等地区广 泛 存 在 。 树 鼩 曾 经 被 划 为 食 虫 目 和 灵 长 目 。 Novacek(1992) 和 Waddell et al(2001)通过哺乳动物 进化关系和重要蛋白氨基酸序列进行的分子进化 分析，认为树鼩是灵长类动物的近亲。中国科学院 昆明动物所细胞库通过与英国剑桥大学等合作，利 用染色体涂色技术建立的染色体同源性图谱表明， 树鼩为单系起源的攀鼩目 (Nie et al, 2008)。由于树 鼩体型小、易饲养等优点，已成为研究多种人类疾 患的良好模型， 包括 HBV、 HCV、 HSV、 CHIK (Walter et al, 1996;Zhao et al, 2002;Darai et al, 1980;Kampen et al, 2002)等。 哺乳动物的 CD3 分子是 T 淋巴细胞受体(TCR) 复合体的重要组成部分，表达于成熟 T 淋巴细胞表 面，由 γ、δ、ε、ζ、η 等 5 种多肽链组成。T 细胞 在胸腺发育过程中， 首先表达 CD3γ、 δ 和 ε 的基因， 经翻译后修饰形成核心结构，在内质网中与后表达 的 TCRα、β 链形成复合物，转移到 T 淋巴细胞表 面 (San et al, 1997) 同时用管家基因 GAPDH (Glyceraldehyde-3-phos- The boxes show the highly conserved cysteine residues in the extracellular domain, the aspartic acid residues in the transmembrane domain and the tyrosine residues in the cytoplasmic domain. ▲, show the SH3 (Src homology region 3) binding motif, the ITAM( immune receptor tyrosine activation motif), the ER motif (Endoplasmic reticulum retention motif) and the SH2 (Src homology region 2) binding motif.…”