Experimental Investigation of the Effects of a Blood Stopper Agent (Ankaferd Blood Stopper) on Bone Surfaces

Abstract

Objective: This study aims to experimentally investigate the efficiency of Ankaferd Blood Stopper (ABS) on early and long-term bone healing and its effects on bone surfaces. Materials and Methods: Thirty adult male Wistar albino rats were used in the study. These rats were randomly divided into three groups, and bilaterally bone defects were created in the femur of each rat. A 3.0-mm-deep monocortical circular defect was created with a 3.0 mm diameter trephine drill on the proximal part of the femur, and 0.05 mL ABS was applied to the experimental group while the control group was left untreated. Group 1, group 2, and group 3 rats were sacrificed on days 7, 28, and 42, respectively. Trabecular bone area (Tb.Ar), medullary bone diameter (Me.Dm), osteoblast area (Ob.Ar), osteoid area (O.Ar) and mineralized bone area (Md.Ar) were examined in the histomorphometric analysis. Also new bone formation was scored according to the histologic evaluation Results: The results showed that while new the to day 7 experimental group showed much more bone formation than the to day 7 control group, there was no significant difference between the to day 28 and day 42 experimental groups and to day 28 and day 42 control groups. Accordingly, ABS applied in bone cavities only had a larger accelerator effect on bone healing for the seventh-day to day 7 experimental group. In clinical observations, no allergic or inflammatory reactions were observed on the skin and other preoperative and postoperative periods. Moreover in, the histomorphometric study, necrotic areas and infection areas were not observed. Conclusion: ABS has an acceleratory effect on the short-term bone healing process and is a reliable agent for routine use. However, its effects on the long-term bone healing process are insignificant. We think that a wide series of research projects are required to confirm the effects of ABS speeding up the healing process in addition to its characteristic as a blood stopping agent. Conflict of interest:None declared.

Ankaferd blood stopper enhances healing after osseous grafting in patients with intrabony periodontal defects

“…Consistent with our results, application of the ABS has been shown to decrease inflammation and necrosis, while increasing new bone formation during the early bone healing period . Likewise, in another experimental study, the findings suggested that ABS had an accelerator effect on short‐term bone healing . ABS has also been shown to exert anti‐infective and healing modulator properties that are also crucial periodontal interventions .…”

“…In addition to the direct usage of ABS in the management of hemorrhagic disorders, off‐label applications have been tested . Such applications demonstrated that ABS could enhance wound healing, infection control, early stage bone tissue healing and formation . These studies demonstrated that the pro‐regenerative and pro‐angiogenic properties of the ABS could be beneficial for a wide spectrum of clinical applications, including the restoration of hard and soft tissues.…”

Investigation of the effect of combined use of alloplastic-based tricalcium phosphate bone graft and antihemorrhagic plant extract (ABS) on bone regeneration in surgically induced bone defects in nondiabetic rats: an experimental animal study

“…All of these plants show their effects on the endothelium, blood cells, angiogenesis, cellular proliferation, vascular dynamics, and mediators. There are many studies reporting that ABS can be used as a hemostatic agent to control hemorrhage after dental extraction, surgical operation, subgingival curettage, and periodontal plastic surgery (24)(25)(26). Studies have also been conducted on the effect of ABS on bone and soft tissue wound healing (26).…”

Effect of Ankaferd blood stopper® on tendon healing: an experimental study in a rat model of Achilles tendon injury

“…They also reported that bone defects treated with ABS were free of inflammation, which is probably related to the anti-inflammatory activity of ABS. Şimşek et al [32] also studied on rat femoral defect models for the effects of ABS on bone defects. They concluded that ABS has an acceleratory effect on short-term bone healing process; however, its effect on long-term bone healing process is insignificant.…”