Experimental investigations of submerged fermentation and synthesis of pectinolytic enzymes by Aspergillus Niger : Effect of inoculum size and age of spores

Mojsov, Kiro

doi:10.15208/ati.2010.13

Cited by 15 publications

(5 citation statements)

References 14 publications

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“…(PSTF1) in submerged fermentation. Similar results were found with pectinase production by Aspergillus niger MK-15 in submerged fermentation, the optimal inoculum concentration was 3 % (3/100 ml) spores and optimal age of spores was 3 days old (Kiro 2010). This showed that the low inoculum size of Fusarium sp.…”

Section: Resultssupporting

confidence: 84%

Bio-catalysis of mango industrial waste by newly isolated Fusarium sp. (PSTF1) for pectinase production

Reddy

Saritha

2015

3 Biotech

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The dried mango fruit processing industrial waste (MIW) used as carbon source for the production of pectinase from fungal strains. Eight fungal strains were isolated from MIW and screened for their ability to produce pectinase by pectin clear zone (PCZ) technique. Fusarium sp. (PSTF1) showed highest PCZ value of 52 mm. The physico-chemical characteristics of the medium were standardized for high production of pectinase. The highest production of pectinase in submerged fermentation observed at temperature—28 °C, pH-6.0, inoculum-size 0.6/25 ml, incubation—72 h, substrate concentration—0.6 g/100 ml, carbon source-fructose (1 %). The effect of different amino acids, vitamins also observed. Under these optimal conditions the highest activity 81.9718 µmol/ml of pectinase was observed. The Fusarium sp. (PSTF1) has been considered as the best pectinase producer in submerged fermentation of MIW. The cheap waste raw material used as best carbon source for high production of high value pectinase.

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Section: Resultssupporting

confidence: 84%

Bio-catalysis of mango industrial waste by newly isolated Fusarium sp. (PSTF1) for pectinase production

Reddy

Saritha

2015

3 Biotech

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“…Results show that inoculum density (final OD 600 ) has a pronounced effect on biosurfactant production. Observations are similar to those reported by Mojsov, Nath and Das and Ghosh . According to Stanbury et al the inoculum or seed culture density influences the duration of the lag phase, specific growth rate, biomass yield, sporulation and quality of the final product.…”

Section: Discussionmentioning

confidence: 99%

Economic production of Bacillus subtilis SPB1 biosurfactant using local agro‐industrial wastes and its application in enhancing solubility of diesel

Mnif

Ellouze-Chaâbouni²,

Ghribi

2012

J of Chemical Tech & Biotech

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BACKGROUND: The present work aimed to optimize a new economic medium for lipopeptide biosurfactant production by Bacillus subtilis SPB1 for application in the environmental field as an enhancer of diesel solubility. Statistical experimental designs and response surface methodology were employed to optimize the medium components. RESULTS: A central composite design was applied to increase the production yield and predict the optimal values of the selected factors. An optimal medium, for biosurfactant production of about 4.5 g L−1, was found to be composed of sesame peel flour (33 g L−1) and diluted tuna fish cooking residue (40%) with an inoculum size of 0.22. Increased inoculum size (final OD600) significantly improved the production yield. The emulsifier produced was demonstrated to be an alternative to chemically synthesized surfactants since it shows high solubilization efficiency towards diesel oil in comparison with SDS and Tween 80. CONCLUSION: Optimization studies led to a strong improvement in production yield. The emulsifier produced, owing its high solubilization capacity and its large tolerance to acidic and alkaline pH values and salinity, shows great potential for use in bioremediation processes to enhance the solubility of hydrophobic compounds. © 2012 Society of Chemical Industry

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“…Dry stalk is usually stored in the form of hay or straw bales for the purpose of feeding animals and other uses. The wheat straw is also used for various purposes after fermentation (Mojsov, 2010, Singh et al, 2009) and pulp industry (Gübitz et al, 1998, Singh et al, 2009) for preparation of biochar (Goodall, 2010), medicine, and bioremediation (Dupont et al, 2006, Javed et al, 2012).…”

Section: Introductionmentioning

confidence: 99%

Nutritional status of different biosolids and their impact on various growth parameters of wheat (Triticum aestivum L.)

Dad

Wahid

Khan

et al. 2019

Saudi Journal of Biological Sciences

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Biosolids can be effectively recycled and applied as soil amendments for agricultural crops because they contain several important micro and macronutrients including nitrogen, phosphorus, potassium, manganese. In the current study, we evaluated the effectiveness of seven biosoilds on different growth parameters of wheat crop. The biosolids used were lime stabilized, composted, liquid mesophilic anaerobically digested (liquid MAD), thermally dried mesophilic anaerobically digested (thermally dried MAD), thermally hydrolyzed mesophilic anaerobically digested (thermally hydrolysed MAD), dewatered mesophilic anaerobically digested (dewatered MAD) and thermally dried raw biosolids. We also analysed biosolids for their nutrient contents before application. The results revealed that different types of biosolids differed in nitrogen and phosphorous contents with highest contents observed in dewatered (5.70% nitrogen, 2.32% phosphorous) and liquid biosolids (2.35% phosphorous). The plant height, plant diameter and dry weight yield of wheat was increased with the increase in concentrations of biosolids. Liquid MAD resulted in maximum plant height of 120.35 ± 3.23, 133.2 ± 3.67 and 147.25 ± 3.11 at 3.33, 6.66 and 9.99 tons/ha concentration. The highest plant diameter was recorded (1.05–1.45 cm) where mineral nitrogen was applied. The study will be helpful in replacing the synthetic fertilizer with biosolids to fulfil the nutritional requirements of agricultural crops.

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Experimental investigations of submerged fermentation and synthesis of pectinolytic enzymes by Aspergillus Niger : Effect of inoculum size and age of spores

Cited by 15 publications

References 14 publications

Bio-catalysis of mango industrial waste by newly isolated Fusarium sp. (PSTF1) for pectinase production

Bio-catalysis of mango industrial waste by newly isolated Fusarium sp. (PSTF1) for pectinase production

Economic production of Bacillus subtilis SPB1 biosurfactant using local agro‐industrial wastes and its application in enhancing solubility of diesel

Nutritional status of different biosolids and their impact on various growth parameters of wheat (Triticum aestivum L.)

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