Experimental models of renal dysfunction in female rats. Functional and histological aspects after unilateral nephrectomy or ligation of right renal vein with kidney preservation

Racanicchi, Ingrid Anita Colombo Wadih Schumann; Oliveira, Ana Beatriz Soares de; Barbieri, Ricardo Augusto; Dellê, Humberto; Duarte, Ivone da Silva; Leme, Pedro Luiz Squilacci

doi:10.1590/s0102-865020150120000006

Cited by 4 publications

(5 citation statements)

References 24 publications

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“…It is a technique that does not involve special costs, with a low impact on the status of the experimental animal. (8,9) We mention the fact that the animals after the surgery will be placed in spaces where a constant temperature of 25 degrees Celsius is ensured, a 12-hour day/night cycle and food and water ad libitum (table no. 1).…”

Section: Resultsmentioning

confidence: 99%

Some Models of Chronic Kidney Disease Induced in the Experimental Animal

Stoica

Gliga

Caldararu

2021

Acta Medica Transilvanica

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Chronic kidney disease (CKD) is one of the clinical features characterized by progressive and irreversible loss of renal function. The incidence of this pathology is constantly increasing globally, due to the growing number of patients diagnosed with diabetes and hypertension, both diseases generating tubular fibrosis and kidney dysfunction. Through experimental models for the production of tubulo-interstitial fibrosis (TIF), we try to understand deeply and comprehensively the main pathogenic mechanisms that govern the onset, progression and worsening of CKD. Understanding the mechanisms underlying the production of this pathology, one can try therapeutic methods to produce an evolutionary slowdown in CKD and also translate the main benefits in clinical practice, based on these experimental models of basic research.

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Section: Resultsmentioning

confidence: 99%

Some Models of Chronic Kidney Disease Induced in the Experimental Animal

Stoica

Gliga

Caldararu

2021

Acta Medica Transilvanica

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“…To further accelerate the decline of renal function, we performed UNx in addition to salt loading. By promoting renal dysfunction via reducing the number of nephrons, UNx and 5/6 nephrectomy in rats and mice are widely used as experimental models of chronic kidney disease (CKD) 25‐27 …”

Section: Discussionmentioning

confidence: 99%

Salt loading with unilateral nephrectomy accelerates decline in glomerular filtration rate in the hypertensive, obese, type 2 diabetic SDT fatty rat model of diabetic kidney disease

Shinozaki

Katayama²,

Yamaguchi³

et al. 2022

Clin Exp Pharma Physio

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For the evaluation of novel therapeutic agents for diabetic kidney disease (DKD), it is desirable to examine their efficacy in animal models by using the glomerular filtration rate (GFR) as an index. For this purpose, animal models that demonstrate a short‐term GFR decline because of disease progression are required. Therefore, we aimed to develop such an animal model of DKD by using obese type 2 diabetic spontaneously diabetic Torii (SDT) fatty rats treated with salt loading by drinking water containing sodium chloride with or without unilateral nephrectomy. As a result, we have found that 0.3% salt loading with unilateral nephrectomy or 0.8% salt loading alone caused a rapid GFR decline, hypertension and rapid development of tubulointerstitial fibrosis. Moreover, the addition of losartan to a mixed diet suppressed the GFR decline in SDT fatty rats treated with 0.3% salt loading with unilateral nephrectomy. These results suggest that the model of SDT fatty rats treated with 0.3% salt loading and unilateral nephrectomy could be used as a hypertensive DKD model for evaluating therapeutic agents based on suppression of GFR decline.

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“…The incidence of CIN dramatically increases in patients with pre-existing renal impairments [24]. Significant alterations in renal function parameters have been observed after unilateral ligation of the renal vein [25]. At the same time, a more marked increase in plasma creatinine levels has been observed in rats with a unilateral ureteral obstruction [26].…”

Section: Discussionmentioning

confidence: 99%

Rho Kinase Inhibitor, Fasudil, Attenuates Contrast‐induced Acute Kidney Injury

Wang

Zhang

Yang

et al. 2017

Basic Clin Pharma Tox

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In this study, we tested the hypothesis that fasudil, a Rho kinase inhibitor, would protect against contrast-induced acute kidney injury (CI-AKI) in a mouse model and attempted to elucidate the mechanism involved. Mice subjected to unilateral ligation of the left anterior renal pedicle were divided into four groups: (1) control group, (2) CI-AKI induced by contrast media (CM group), (3) CI-AKI plus low-dose fasudil (LD group) and (4) CI-AKI plus high-dose fasudil (HD group). Animals from groups 2-4 received iodixanol (4.0 g iodine/kg), and the control group received saline. At 12, 2 hr before iodixanol injection and 4 hr after iodixanol administration, the animals in groups 3-4 received 3 or 10 mg/kg fasudil, respectively. Renal blood flow, renal function parameters, kidney histology and the expression of proteins that regulates apoptosis and inflammation were determined 24 hr later. Fasudil treatment notably ameliorated contrast medium-induced medullary damage, restored renal function, suppressed renal tubular apoptosis, ameliorated redox imbalance and DNA damage. Fasudil had a nephroprotective effect that was partially attributed to its anti-inflammatory, anti-apoptotic and antioxidant effects of inhibiting the Rho/ROCK pathway.

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Experimental models of renal dysfunction in female rats. Functional and histological aspects after unilateral nephrectomy or ligation of right renal vein with kidney preservation

Cited by 4 publications

References 24 publications

Some Models of Chronic Kidney Disease Induced in the Experimental Animal

Some Models of Chronic Kidney Disease Induced in the Experimental Animal

Salt loading with unilateral nephrectomy accelerates decline in glomerular filtration rate in the hypertensive, obese, type 2 diabetic SDT fatty rat model of diabetic kidney disease

Rho Kinase Inhibitor, Fasudil, Attenuates Contrast‐induced Acute Kidney Injury

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