Experimental Models to Study Skin Wound Healing with a Focus on Angiogenesis

Grambow, Eberhard; Sorg, Heiko; Sorg, Christian G G; Strüder, Daniel

doi:10.3390/medsci9030055

Cited by 26 publications

(26 citation statements)

References 131 publications

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“…The healing process can be delayed due to diverse pathologies among different wounds, such as post-operative wounds, burns, chronic ulcers, etc. ( Grambow et al, 2021 ). Furthermore, age, disease, infection, stress, and nutrition also contribute to a delayed healing process.…”

Section: Introductionmentioning

confidence: 99%

Design and evaluation of sodium alginate-based hydrogel dressings containing Betula utilis extract for cutaneous wound healing

Ishfaq

Khan

Khalid

et al. 2023

Front. Bioeng. Biotechnol.

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Traditional wound dressings have a limited capacity to absorb exudates, are permeable to microbes, and may adhere to wounds, which leads to secondary injuries. Hydrogels are promising alternative dressings to overcome the above challenges. In this study, we developed sodium alginate-based hydrogel films loaded with Betula utilis bark extract. These films were prepared via solvent-casting crosslinking method and evaluated for wound healing activity. Prepared films were 0.05–0.083 mm thick, flexible with folding endurance ranging from 197–203 folds, which indicates good physical properties. Optimized formulations exhibited successful loading of extract in the film matrix without any interaction as confirmed by FTIR. Maximum zone of inhibition against Gram-positive and Gram-negative bacteria was achieved by optimum formulation (B6), i.e., 19 mm and 9 mm, respectively, with > 90% scavenging activity. Furthermore, this optimum formulation (B6) was able to achieve 93% wound contraction in rats. Histograms of the optimized formulation treated group also revealed complete reepithelization of wounds. Conclusively, our extract-loaded hydrogel dressing successfully demonstrated its potential for cutaneous wound healing.

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Section: Introductionmentioning

confidence: 99%

Design and evaluation of sodium alginate-based hydrogel dressings containing Betula utilis extract for cutaneous wound healing

Ishfaq

Khan

Khalid

et al. 2023

Front. Bioeng. Biotechnol.

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“…Although a tendency of a prolonged release can be observed (Figure 7), the differences did not reach statistical significance (p = 0.09 [ROI 1]; p = 0.134 [ ). Considering the long half-life of both the encapsulated substance as well as the albumin nanocarriers, based on the data obtained in our experiments, a definite and well-founded statement regarding the influence of the albumin nanocarriers on the release kinetics of Etanercept cannot be made and is ultimately impaired by the variation in fluorescence intensity and the limited observation period, when using the dorsal skinfold chamber model [59,60]. Models that allow a longer observational timeframe might be more suited to properly investigate differences in release kinetics in this specific case.…”

Section: Discussionmentioning

confidence: 90%

Surface Modification of Porous Polyethylene Implants with an Albumin-Based Nanocarrier-Release System

et al. 2021

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Background: Porous polyethylene (PPE) implants are used for the reconstruction of tissue defects but have a risk of rejection in case of insufficient ingrowth into the host tissue. Various growth factors can promote implant ingrowth, yet a long-term gradient is a prerequisite for the mediation of these effects. As modification of the implant surface with nanocarriers may facilitate a long-term gradient by sustained factor release, implants modified with crosslinked albumin nanocarriers were evaluated in vivo. Methods: Nanocarriers from murine serum albumin (MSA) were prepared by an inverse miniemulsion technique encapsulating either a low- or high-molar mass fluorescent cargo. PPE implants were subsequently coated with these nanocarriers. In control cohorts, the implant was coated with the homologue non-encapsulated cargo substance by dip coating. Implants were consequently analyzed in vivo using repetitive fluorescence microscopy utilizing the dorsal skinfold chamber in mice for ten days post implantation. Results: Implant-modification with MSA nanocarriers significantly prolonged the presence of the encapsulated small molecules while macromolecules were detectable during the investigated timeframe regardless of the form of application. Conclusions: Surface modification of PPE implants with MSA nanocarriers results in the alternation of release kinetics especially when small molecular substances are used and therefore allows a prolonged factor release for the promotion of implant integration.

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“…Wound healing is a successive process requiring multiple biological pathways and intricate biochemical cascade reactions to be activated and synchronized to recover tissue integrity [ 137 ]. Hydrogels are an ideal dressing for the wound management market in protecting the wound against bacterial infection, but impaired vessel formation and undermined cell proliferation make chronic wounds stall in the inflammatory phase [ 138 ]. Thus, developing hydrogels with capacities to promote tissue regeneration is highly desired.…”

Section: Application Of Mesoporous Material-loaded Composite Hydrogelsmentioning

confidence: 99%

Mesoporous Materials Make Hydrogels More Powerful in Biomedicine

Chen

Qiu

Xia

et al. 2023

Gels

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Scientists have been attempting to improve the properties of mesoporous materials and expand their application since the 1990s, and the combination with hydrogels, macromolecular biological materials, is one of the research focuses currently. Uniform mesoporous structure, high specific surface area, good biocompatibility, and biodegradability make the combined use of mesoporous materials more suitable for the sustained release of loaded drugs than single hydrogels. As a joint result, they can achieve tumor targeting, tumor environment stimulation responsiveness, and multiple therapeutic platforms such as photothermal therapy and photodynamic therapy. Due to the photothermal conversion ability, mesoporous materials can significantly improve the antibacterial ability of hydrogels and offer a novel photocatalytic antibacterial mode. In bone repair systems, mesoporous materials remarkably strengthen the mineralization and mechanical properties of hydrogels, aside from being used as drug carriers to load and release various bioactivators to promote osteogenesis. In hemostasis, mesoporous materials greatly elevate the water absorption rate of hydrogels, enhance the mechanical strength of the blood clot, and dramatically shorten the bleeding time. As for wound healing and tissue regeneration, incorporating mesoporous materials can be promising for enhancing vessel formation and cell proliferation of hydrogels. In this paper, we introduce the classification and preparation methods of mesoporous material-loaded composite hydrogels and highlight the applications of composite hydrogels in drug delivery, tumor therapy, antibacterial treatment, osteogenesis, hemostasis, and wound healing. We also summarize the latest research progress and point out future research directions. After searching, no research reporting these contents was found.

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Experimental Models to Study Skin Wound Healing with a Focus on Angiogenesis

Cited by 26 publications

References 131 publications

Design and evaluation of sodium alginate-based hydrogel dressings containing Betula utilis extract for cutaneous wound healing

Design and evaluation of sodium alginate-based hydrogel dressings containing Betula utilis extract for cutaneous wound healing

Surface Modification of Porous Polyethylene Implants with an Albumin-Based Nanocarrier-Release System

Mesoporous Materials Make Hydrogels More Powerful in Biomedicine

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