We conducted experimental investigations on the effect of stable thermal conditions on rough-wall boundary layers, with a specific focus on their response to abrupt increases in surface roughness. For stably stratified boundary layers, a new analytical relation between the skin-friction coefficient,
$C_f$
, and the displacement thickness was proposed. Following the sharp roughness change, the overshoot in
$C_f$
is slightly enhanced in stably stratified layers when compared with that of neutral boundary layers. Regarding the velocity defect law, we found that the displacement thickness multiplied by
$\sqrt{2/C_f}$
, performs better than the boundary layer thickness alone when describing the similarity within internal boundary layers for both neutral and stable cases. A non-adjusted region located just beneath the upper edge of the internal boundary layer was observed, with large magnitudes of skewness and kurtosis of streamwise and wall-normal velocity fluctuations for both neutral and stable cases. At a fixed wall-normal location, the greater the thermal stratification, the greater the magnitudes of skewness and kurtosis. Quadrant analysis revealed that the non-adjusted region is characterised by an enhancement/reduction of ejection/sweep events, particularly for stably stratified boundary layers. Spatially, these ejections correspond well with peaks of kurtosis, exhibit stronger intensity and occur more frequently following the abrupt change in surface conditions.