Experimental toxocariosis in BALB/c mice: Effect of the inoculation dose on brain and eye involvement

Ollero, M. D.; Fenoy, S.; Cuéllar, C.; Guillén, J.L.; Águila, C. del

doi:10.1016/j.actatropica.2007.11.001

Cited by 24 publications

(22 citation statements)

References 35 publications

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“…This phenomenon has also been observed in murines, which are considered the experimental model for human toxocariasis. In mice, seroconversion was observed at 7 (OLLERO et al, 2008), 14 (KOLBEKOVÁ et al, 2011 and 23 DPI (LESCANO et al, 2012).…”

Section: Discussionmentioning

confidence: 99%

“…There are a number of factors that may determine the pattern of immune response and pathogenesis in paratenic hosts, including individual characteristics (CUELLAR et al, 2001), the infective dose and number of reinfections (KAYES et al, 1985;OLLERO et al, 2008). Our findings reinforce the statement that individuality may explain a difference in responsiveness to antigens by each individual animal, since some of the rabbits (41.7%) seroconverted in the early days of infection (14 DPI), followed by observation of seroconversion at different time points, including one registered at 60 DPI.…”

Section: Discussionmentioning

confidence: 99%

“…The present study has some limitations. Several experimental studies have evaluated the subclasses of immunoglobulins involved in toxocariasis (KAYES et al, 1985;OLLERO et al, 2008). We used the indirect ELISA based on the detection of total IgG anti-Toxocara, which is currently the standard serological method for the diagnosis of human toxocariasis (RUBINSKY-ELEFANT et al, 2010;MACPHERSON, 2013).…”

Section: Discussionmentioning

confidence: 99%

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Kinetics and avidity of anti-Toxocara antibodies (IgG) in rabbits experimentally infected with Toxocara canis

Bin

Santarém

Laposy

et al. 2015

Rev. Bras. Parasitol. Vet.

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An evaluation was made of the kinetics and avidity of anti-Toxocara antibodies (IgG) in rabbits experimentally infected with embryonated Toxocara canis eggs. Seventeen four month old New Zealand White rabbits were distributed into two groups. In the experimental group, twelve rabbits were infected orally with 1,000 embryonated T. canis eggs. A second group (n = 5), uninfected, was used as a control. Serum samples were collected for analysis on days 7, 14, 21, 28 and 60 post-infection (DPI). An indirect ELISA test was performed to evaluate the reactivity index (RI) of IgG anti-T. canis antibodies and to calculate the avidity index (AI). The animals showed seroconversion from the 14 th DPI, with high AI (over 50%) except for one animal, which presented an intermediate AI. At 60 DPI, all the animals were seropositive and maintained a high AI. The data indicated that specific IgG antibodies formed early (14 DPI) in rabbits infected with T. canis, with a high avidity index that persisted throughout the course of the infection.

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Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

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Kinetics and avidity of anti-Toxocara antibodies (IgG) in rabbits experimentally infected with Toxocara canis

Bin

Santarém

Laposy

et al. 2015

Rev. Bras. Parasitol. Vet.

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“…Low dose mice mimic the patterns observed in wild mice, whereas higher doses are frequently selected in experimental infections in order to achieve marked brain involvement and accumulation over time. Ollero and colleagues demonstrated that multiple doses enhanced the number of larvae recovered from the brains of BALB/c mice (Ollero et al, 2008).…”

Section: The Toxocara Mouse Model: Brain Involvement Accumulation Anmentioning

confidence: 99%

The significance of cerebral toxocariasis: a model system for exploring the link between brain involvement, behaviour and the immune response

Holland

Hamilton

2013

Journal of Experimental Biology

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SummaryToxocara canis is a parasitic nematode that infects canines worldwide, and as a consequence of the widespread environmental dissemination of its ova in host faeces, other abnormal hosts including mice and humans are exposed to infection. In such abnormal or paratenic hosts, the immature third-stage larvae undergo a somatic migration through the organs of the body but fail to reach maturity as adult worms in the intestine. The presence of the migrating larvae contributes to pathology that is dependent upon the intensity of infection and the location of the larvae. A phenomenon of potential public health significance in humans and of ecological significance in mice is that T. canis larvae exhibit neurotrophic behaviour, which results in a greater concentration of parasites in the brain, as infection progresses. Toxocara larval burdens vary between individual outbred mice receiving the same inocula, suggesting a role for immunity in the establishment of cerebral infection. Although the systemic immune response to T. canis has been widely reported, the immune response in the brain has received little attention. Differential cytokine expression and other brain injury-associated biomarkers have been observed in infected versus uninfected outbred and inbred mice. Preliminary data have also suggested a possible link between significant memory impairment and cytokine production associated with T. canis infection. Mice provide a useful, replicable animal model with significant applicability and ease of manipulation. Understanding the cerebral host-parasite relationship may shed some light on the cryptic symptoms of human infection where patients often present with other CNS disorders such as epilepsy and mental retardation.

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“…Hamsters were housed in the animal facility of the Institute of Tropical Medicine, S. Paulo University (IMT), and were maintained on commercial pellet food and water ad libitum. Six groups of five male hamsters each (mean weight, 180 g) were orally exposed to 750 embryonated eggs by gavage (Ollero et al 2008). Hamsters were sacrificed at 4, 6, 10, 15, 30, and 45 days postinfection, and liver, lung, and kidney fragments were routinely fixed in 10 % buffered formalin (pH 7.2), processed, embedded in paraffin, sectioned at 3-4-μm thickness, and stained with hematoxylin and eosin.…”

Section: Methodsmentioning

confidence: 99%

Toxocaríase experimental em hamster

Silva¹

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A Deus por sempre iluminar meu caminho, e permitir a minha trajetória.Ao Prof. Thales de Brito, por sua dedicação incansável a esse trabalho, pelos anos de convivência que me permitiram testemunhar seu amor pela ciência e seu entusiasmo pela vida.Ao Prof. Pedro Paulo Chieffi, pelos ensinamentos, sugestões e apoio, que tanto contribuíram para a realização desse trabalho.A Profa. Mirian Nacagami Sotto, pelo incentivo, apoio e amizade.Ao Prof. Edécio Cunha-Neto, por ter me recebido em seu Laboratório de Imunologia do INCOR, onde foi realizado o RT-PCR, e por sua contribuição na interpretação dos resultados.

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Experimental toxocariosis in BALB/c mice: Effect of the inoculation dose on brain and eye involvement

Cited by 24 publications

References 35 publications

Kinetics and avidity of anti-Toxocara antibodies (IgG) in rabbits experimentally infected with Toxocara canis

Kinetics and avidity of anti-Toxocara antibodies (IgG) in rabbits experimentally infected with Toxocara canis

The significance of cerebral toxocariasis: a model system for exploring the link between brain involvement, behaviour and the immune response

Toxocaríase experimental em hamster

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