Experimental transmission of Macrobrachium rosenbergii nodavirus (MrNV) and extra small virus (XSV) in three species of marine shrimp (Penaeus indicus, Penaeus japonicus and Penaeus monodon)

Sudhakaran, R.; Musthaq, S. Syed; Haribabu, P.; Mukherjee, Susmita; Gopal, C.; Hameed, A.S. Sahul

doi:10.1016/j.aquaculture.2006.02.053

Cited by 44 publications

(31 citation statements)

References 13 publications

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“…The susceptibility of three species of marine shrimp (Penaeus indicus, P. japonicus, and P. monodon) to MrNV and XSV was tested by oral route and intramuscular injection [27]. The viruses failed to produce mortality in the shrimp but RT-PCR analysis revealed the presence of MrNV and XSV in the gills, abdominal muscle, stomach, intestine, and hemolymph of shrimp injected with the viruses.…”

Section: Host Susceptibilitymentioning

confidence: 99%

White Tail Disease of Freshwater Prawn, Macrobrachium rosenbergii

Hameed¹,

Bonami

2012

Indian J. Virol.

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Macrobrachium rosenbergii is the most important cultured freshwater prawn in the world and it is now farmed on a large scale in many countries. Generally, freshwater prawn is considered to be tolerant to diseases but a disease of viral origin is responsible for severe mortalities in larval, post-larval and juvenile stages of prawn. This viral infection namely white tail disease (WTD) was reported in the island of Guadeloupe in 1995 and later in Martinique (FrenchWest Indies) in Taiwan, the People's Republic of China, India, Thailand, Australia and Malaysia. Two viruses, Macrobrachium rosenbergii nodavirus (MrNV) and extra small virus-like particle (XSV) have been identified as causative agents of WTD. MrNV is a small icosahedral non-enveloped particle, 26-27 nm in diameter, identified in the cytoplasm of connective cells. XSV is also an icosahedral virus and 15 nm in diameter. Clinical signs observed in the infected animals include lethargy, opaqueness of the abdominal muscle, degeneration of the telson and uropods, and up to 100 % within 4 days. The available diagnostic methods to detect WTD include RT-PCR, dot-blot hybridization, in situ hybridization and ELISA. In experimental infection, these viruses caused 100 % mortality in post-larvae but failed to cause mortality in adult prawns. The reported hosts for these viruses include marine shrimp, Artemia and aquatic insects. Experiments were carried out to determine the possibility of vertical transmission of MrNV and XSV in M. rosenbergii. The results indicate that WTD may be transferred from infected brooders to their offspring during spawning. Replication of MrNV and XSV was investigated in apparently healthy C6/36 Aedes albopictus and SSN-1 cell lines. The results revealed that C6/36 and SSN-1cells were susceptible to these viruses. No work has been carried out on control and prevention of WTD and dsRNA against protein B2 produced RNAi that was able to functionally prevent and reduce mortality in WTD-infected redclaw crayfish.

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Section: Host Susceptibilitymentioning

confidence: 99%

White Tail Disease of Freshwater Prawn, Macrobrachium rosenbergii

Hameed¹,

Bonami

2012

Indian J. Virol.

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“…MrNV is the causative agent of white tail disease in the freshwater prawn Macrobrachium rosenbergii [36] and apparently does not cause disease in juvenile marine shrimp [37] but may do so in larval stages [38]. Thus, based on our small sample, interaction between Lamr and viral capsid/envelope proteins appears to be generally restricted to RNA viruses but specific to only some.…”

Section: Interaction Between Lamr and Shrimp Virusesmentioning

confidence: 88%

Shrimp laminin receptor binds with capsid proteins of two additional shrimp RNA viruses YHV and IMNV

Busayarat

Senapin

Tonganunt

et al. 2011

Fish & Shellfish Immunology

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“…data). For MrNV, several species of marine penaeid shrimp (Penaeus indicus, P. japonicus, and P. monodon) have been identified as reservoir hosts (Sudhakaran et al 2006). Recently, post-larval P. monodon and P. indicus, both displaying gross signs of whitish muscle, were found to be infected with MrNV and XSV by RT-PCR (Ravi et al 2009).…”

Section: Discussionmentioning

confidence: 99%

Ultrastructural and sequence characterization of Penaeus vannamei nodavirus (PvNV) from Belize

Tang¹,

Pantoja²,

Redman³

et al. 2011

Dis. Aquat. Org.

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The Penaeus vannamei nodavirus (PvNV), which causes muscle necrosis in Penaeus vannamei from Belize, was identified in 2005. Infected shrimp show clinical signs of white, opaque lesions in the tail muscle. Under transmission electron microscopy, the infected cells exhibit increases in various organelles, including mitochondria, Golgi stacks, and rough endoplasmic reticulum. Cytoplasmic inclusions containing para-crystalline arrays of virions were visualized. The viral particle is spherical in shape and 19 to 27 nm in diameter. A cDNA library was constructed from total RNA extracted from infected shrimp. Through nucleotide sequencing from the cDNA clones and northern blot hybridization, the PvNV genome was shown to consist of 2 segments: RNA1 (3111 bp) and RNA2 (1183 bp). RNA1 contains 2 overlapped open reading frames (ORF A and B), which may encode a RNA-dependent RNA polymerase (RdRp) and a B2 protein, respectively. RNA2 contains a single ORF that may encode the viral capsid protein. Sequence analyses showed the presence of 4 RdRp characteristic motifs and 2 conserved domains (RNA-binding B2 protein and viral coat protein) in the PvNV genome. Phylo genetic analysis based on the translated amino acid sequence of the RdRp reveals that PvNV is a member of the genus Alphanodavirus and closely related to Macrobrachium rosenbergii nodavirus (MrNV). In a study investigating potential PvNV vectors, we monitored the presence of PvNV by RT-PCR in seabird feces and various aquatic organisms collected around a shrimp farm in Belize. PvNV was detected in mosquitofish, seabird feces, barnacles, and zooplankton, suggesting that PvNV can be spread via these carriers. KEY WORDS: Penaeus vannamei nodavirus · PvNV · Ultrastructure · Genomic sequencing · Mechanical carriersResale or republication not permitted without written consent of the publisher RNA-dependent RNA polymerase (RdRp), and RNA2 encodes the capsid protein. RNA3 encodes 1 or 2 small proteins (designated B1 and B2). The function of B1 is not known, but B2 is a double-stranded RNA-binding protein described in other noda viruses, such as Flock House virus (FHV) and Greasy grouper nervous necrosis virus (GGNNV), to act as a suppressor against host antiviral RNA-silencing res ponse (Li et al. 2002, Lingel et al. 2005, Fenner et al. 2006. However, whether the B2 of PvNV functions similarly in the shrimp cells is not yet known.In the present study, we examined the morphogenesis of PvNV under transmission electron microscopy (TEM), sequenced its genome (both RNA1 and RNA2), and determined the taxonomic status of the virus. In addition, we investigated potential carriers of PvNV collected from shrimp ponds. MATERIALS AND METHODSPvNV-infected shrimp. Shrimp taxonomy was according to Holthius (1980). The PvNV-infected Penaeus vannamei used in this study were generated by laboratory infection from an isolate obtained from a farm in Belize in 2005. The preparation of inoculum and laboratory infection procedure were described by Tang et al (2007).Electron microscopy s...

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Experimental transmission of Macrobrachium rosenbergii nodavirus (MrNV) and extra small virus (XSV) in three species of marine shrimp (Penaeus indicus, Penaeus japonicus and Penaeus monodon)

Cited by 44 publications

References 13 publications

White Tail Disease of Freshwater Prawn, Macrobrachium rosenbergii

White Tail Disease of Freshwater Prawn, Macrobrachium rosenbergii

Shrimp laminin receptor binds with capsid proteins of two additional shrimp RNA viruses YHV and IMNV

Ultrastructural and sequence characterization of Penaeus vannamei nodavirus (PvNV) from Belize

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