Explant type and natural ventilation systems influence growth and content of carvacrol and thymol of Lippia gracilis Schauer

Lazzarini, Luiz Eduardo Santos; Bertolucci, Suzan Kelly Vilela; Carvalho, Alexandre Alves de; Santiago, Alexsandro Carvalho; Pacheco, Fernanda Ventorim; Yücesan, Buhara; Pinto, José Eduardo Brasil Pereira

doi:10.1007/s11240-018-01548-5

Cited by 19 publications

(10 citation statements)

References 41 publications

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“…Ziska et al (2008) observed a progressive increase in the SLA of Papaver setigerum with increasing CO2 concentration. Lazzarini et al (2019) reported no difference in SLA in Lippia gracilis. Benicasa (2003) reported that if we consider weight as an expression of leaf volume, the inverse of SLA, which is known as SLW, indicates leaf thickness.…”

Section: In Vitro Growthmentioning

confidence: 94%

“…Thus, the plantlets achieved a higher dry weight for all organs (leaf, stem, and root). Lazzarini et al (2019) observed that the lower the LAR was, the higher the efficiency of plant dry weight production was. LWR is the weight of dry weight retained in the leaves and the weight of dry weight stored in the whole plant; in this case, LWR was defined as LDW + SDW.…”

Section: In Vitro Growthmentioning

confidence: 99%

“…Another problem that is minimized is the accumulation of ethylene gas, which causes harmful physiological changes to plantlets (Kozai & Kubota, 2001). Lazzarini et al (2019) reported that an alternative system with 4 membranes and the type of explant significantly influenced the growth and volatile fraction of Lippia gracilis. Rocha et al (2022) arvensis and M. viridis ((de Oliveira et al, 2021).…”

Section: In Vitro Growthmentioning

confidence: 99%

“…(Lamiacea), while the use of 4 membranes favored the accumulation of carvacrol in this species (Silva et al, 2017). In a study with Lippia gracilis (Schauer) (Vebenaceae), the use of AMS resulted in greater dry weight accumulation, reductions in γ-terpinene and ρ-cymene metabolites and increases in thymol and carvacrol in plantlets grown for 35 days in a cultivation system containing 4 porous membranes (Lazzarini et al, 2019).…”

Section: Introductionmentioning

confidence: 98%

“…The use of alternative membrane systems (AMSs) has been shown to be promising in the optimization of the growth of medicinal plants in vitro (de Oliveira et al, 2021;Lazzarini et al, 2019;Rocha et al, 2022;Silva et al, 2016;Silva et al, 2017). The use of AMSs allows the entry of CO2 into the microenvironment and improves photosynthetic capacity while maintaining asepsis and reducing the relative humidity and ethylene concentration in the flasks, enabling the production of Research, Society and Development, v. 11, n. 12, e203111234446, 2022 (CC BY 4.0) | ISSN 2525-3409 | DOI: http://dx.doi.org/10.33448/rsd-v11i12.34446 more vigorous plants with greater survival in the acclimatization period (Saldanha et al, 2012).…”

Section: Introductionmentioning

confidence: 99%

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Different types of explants and natural ventilation systems influence the accumulation of dry weight and of total phenolic compounds in Aloysia gratissima (Verbenaceae)

Coelho

Leite²,

Santos³

et al. 2022

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The objective of this study was to evaluate the effect of the use of different natural ventilation systems on the growth and accumulation of total phenolic compounds in Aloysia gratissima. Nodal and apical segments with 1 pair of leaves were inoculated in flasks containing ½ MS culture medium without sucrose and supplemented with 0.1 mg L-1 indole-butyric acid. The cultivation systems included a conventional system (NMS) and alternative membrane systems with 1 (AMS1), 2 (AMS2) and 4 (AMS4) low-cost membranes. After 30 days, the in vitro growth, leaf area, dry weight and accumulation of phenolic compounds were evaluated. Plantlets from apical segments showed superior results in all variables analyzed. The use of the NMS compromised plantlet growth. However, improved results were obtained with the use of porous membranes, with the best growth observed in AMS4. The leaf area of plantlets originating from apical segments was 3.03 times greater than that of plantlets from nodal segments. Plantlets in the NMS had the lowest values of leaf area, root length, number of leaves and dry weight. However, the use of membranes allowed higher growth. The AMS4 treatment increased the leaf dry weight accumulation by 6.13-fold compared to that obtained with the NMS treatment. The accumulation of total phenolic compounds increased with the use of greater numbers of porous membranes. The use of apical segments and lids with 4 porous membranes is recommended for micropropagation of the species. The use of an alternative membrane system positively influences the accumulation of total phenolic compounds.

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Section: In Vitro Growthmentioning

confidence: 94%

Section: In Vitro Growthmentioning

confidence: 99%