Exploring effects of betaine-homocysteine methyltransferase (BHMT) gene polymorphisms on fatty acid traits and  cholesterol in sheep

Munyaneza, Jean Pierre; Gunawan, Asep; Noor, Ronny Rachman

doi:10.14710/jitaa.44.3.243-251

Cited by 11 publications

(17 citation statements)

References 15 publications

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“…Overall, the result showed the average value of SFA in lamb meat was higher than UFA (PUFA+MUFA) in all genotypes. This result agrees with the current study by Munyaneza et al (2019) that generally fatty composition of lamb meat is dominated by SFA. MUFA could reduce LDL cholesterol (low-density lipoprotein) and increase HDL cholesterol (high-density lipoprotein) levels.…”

Section: Discussionsupporting

confidence: 94%

The Polymorphism and Expression of CYP2E1 Gene and Its Relation to Carcass and Meat Quality of Indonesian Lamb

Harahap

Noor

Gunawan

2021

Trop. Anim. Sci. J.

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Section: Discussionsupporting

confidence: 94%

The Polymorphism and Expression of CYP2E1 Gene and Its Relation to Carcass and Meat Quality of Indonesian Lamb

Harahap

Noor

Gunawan

2021

Trop. Anim. Sci. J.

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“…The TT genotype was relatively rare in the populations (JFT, JTT, BCS, CAS, GS, and JS). This finding is similar to an association study which has used a different gene (diacylglycerol O-acyltransferase 1 [DGAT1]) in JFT, JTT, BCS, CAS, and GCS [27], however it is inconsistent with the previous study performed for a different gene (betaine-homocysteine S-methyltransferase [BHMT]) in JFT, JTT, BCS, CAS, and GCS [28]. Munyaneza et al [28] reported that TT genotypes are more frequent than CC and CT genotypes.…”

Section: Discussionsupporting

confidence: 47%

Association study and expression analysis of olfactomedin like 3 gene related to meat quality, carcass characteristics, retail meat cut, and fatty acid composition in sheep

et al. 2022

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Objective: The objective of this study was to identify polymorphism in olfactomedin like 3 (OLFML3) gene, and association analysis with meat quality, carcass characteristics, retail meat cut, and fatty acid composition in sheep, and expression quantification of OLFML3 gene in phenotypically divergent sheep.Methods: A total of 328 rams at the age of 10 to 12 months with an average body weight of 26.13 kg were used. A novel polymorphism was identified using high-throughput sequencing in sheep and genotyping of OLFML3 polymorphism was performed using polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP). Among 328 rams, 100 rams representing various sheep genotypes were used for association study and proc general linear model was used to analyse association between genotypes and phenotypic traits. Quantitative real-time polymerase chain reaction (qRT-PCR) was used for the expression analysis of OLFML3 mRNA in phenotypically divergent sheep population.Results: The findings revealed a novel polymorphism in the OLFML3 gene (g.90317673 C>T). The OLFML3 gene revealed three genotypes: CC, CT, and TT. The single nucleotide polymorphism (SNP) was found to be significantly (p<0.05) associated with meat quality traits such as tenderness and cooking loss; carcass characteristics such as carcass length; retail meat cut such as pelvic fat in leg, intramuscular fat in loin and tenderloin, muscle in flank and shank; fatty acids composition such as tridecanoic acid (C13:0), palmitoleic acid (C16:1), heptadecanoic acid (C17:0), ginkgolic acid (C17:1), linolenic acid (C18:3n3), arachidic acid (C20:0), eicosenoic acid (C20:1), arachidonic acid (C20:4n6), heneicosylic acid (C21:0), and nervonic acid (C24:1). The TT genotype was associated with higher level of meat quality, carcass characteristics, retail meat cut, and some fatty acids composition. However, the mRNA expression analysis was not different among genotypes.Conclusion: The OLFML3 gene could be a potential putative candidate for selecting higher quality sheep meat, carcass characteristics, retail meat cuts, and fatty acid composition in sheep.

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“…The HSD17β13 gene of SNP g.101 689 913 C>T had no significant association with flavor and odor (Table 5). In Indonesian lamb quality, several genes that affected flavor odor and fatty acid composition were APOA5 (Gunawan et al, 2018), CYP2A6 (Listyarini et al, 2018), BHMT (Munyaneza et al, 2019), and CYP2E1 (Harahap et al, 2020).…”

Section: Association With Flavor Odor and Fatty Acid Compositionmentioning

confidence: 99%

Polymorphism and Expression of HSD17β13 Gene and Its Association with Lamb Quality of Indonesian Sheep

Harahap

Noor

Gunawan

2021

JAP

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HSD17β13 (17beta 13-Hydroxysteroid dehydrogenases) are important enzymes in steroid metabolism. This study investigated the polymorphisms and expression of the HSD17β13 gene in lamb quality traits in Indonesian sheep. A total of 200 of rams of seven breeds administered in the study were Javanese fat-tailed (JFT), Javanese thin-tailed (JTT), Garut sheep (GS), Jonggol sheep (JS), Garut composite sheep (GCS), Compass agrinac sheep (CAS), Barbados cross sheep (BCS) aged 10-12 months and weighed 20-30 kg. The Polymerase Chain Reaction-Restriction Fragment Length Polymorphism (PCR-RFLP) and MspI enzyme restriction were performed to identify the polymorphism of the HSD17β13 gene. The lamb quality traits were measured for physical quality, flavor, odor, and fatty acid composition. The results showed that the HSD17β13 gene was polymorphic and had three genotypes i.e., CC, CT, and TT. The highest genotype frequency was the CC when compared to the other genotypes. The allele frequency of HSD17β13 gene was not in Hardy Weinberg Equilibrium. The polymorphism of HSD17β13 gene was significantly (P<0.05) associated with the fatty acid composition and Saturated Fatty Acid (SFA), i.e., tridecanoic acid (C13:0), arachidic acid (C20:0), henecosanoic acid (C21:0), and tricosanoic acid (C23:0). The polymorphism was also significantly associated with the Monounsaturated Fatty Acid (MUFA) i.e. ginkgoleic acid (C17:1) and nervonoic acid (C24:1), and Polyunsaturated Fatty Acid (PUFA) i.e. eicosedienoic acid (C20:2). The level of expression of the HSD17β13 gene based on quantitative real time-PCR analysis results was not significantly different (P>0.05) among genotypes for lamb quality traits. It can be concluded that the polymorphism of HSD17β13 gene was associated with the fatty acid composition of lamb in Indonesian sheep.

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Exploring effects of betaine-homocysteine methyltransferase (BHMT) gene polymorphisms on fatty acid traits and cholesterol in sheep

Cited by 11 publications

References 15 publications

The Polymorphism and Expression of CYP2E1 Gene and Its Relation to Carcass and Meat Quality of Indonesian Lamb

The Polymorphism and Expression of CYP2E1 Gene and Its Relation to Carcass and Meat Quality of Indonesian Lamb

Association study and expression analysis of olfactomedin like 3 gene related to meat quality, carcass characteristics, retail meat cut, and fatty acid composition in sheep

Polymorphism and Expression of HSD17β13 Gene and Its Association with Lamb Quality of Indonesian Sheep

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