Outcome-based education (OBE) has changed the previous teacher-centered education model. This study was performed to evaluate the effect of practice of blended teaching mode of emergency first aid specialty based on OBE concept in teaching. In this study, 23 undergraduate students (observation group) at the level of 2017 and 23 undergraduate students (control group) at the level of 2016 majoring in emergency medicine who take part in clinical practice were selected as the study subjects. By setting the expected learning achievement goals in 6 aspects, the mixed teaching method is used to reorganize teaching content and carry out teaching evaluation, and a mixed OBE concept teaching model is constructed and implemented. The number of high scoring students with a score above 90, the number of pass subjects, and the mean score at the class level were significantly improved compared with those who practiced the traditional teaching mode. At the 3 time points of pre internship, in practice and after post internship, the achievement of the 6 expected learning outcomes (19.92 ± 3.68, 23.89 ± 2.93, 25.44 ± 3.33, F = 77.87, P < .001) and critical thinking abilities (283.39 ± 25.84, 287.43 ± 24.33, 300.07 ± 23.87, F = 49.82, P < .001) were significantly improved. Blended teaching mode of emergency first aid specialty based on OBE concept significantly improved education.