Exploring Living Multicellular Organisms, Organs, and Tissues Using Microfluidic Systems

Sivagnanam, Venkataragavalu; Gijs, Martin A. M.

doi:10.1021/cr200432q

Cited by 66 publications

(48 citation statements)

References 216 publications

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“…Microfluidic cell culture arrays have been extended to small multicellular organisms 66 to study developmental biology 67,68 and as platforms for high-throughput testing in vivo 69 . Using a single microfluidic array, researchers grew and monitored 64 Arabidopsis thaliana seedlings expressing 12 different transgenic reporter lines.…”

Section: Live-cell Imagingmentioning

confidence: 99%

Microfluidics: reframing biological enquiry

Duncombe

Tentori

Herr

2015

Nat Rev Mol Cell Biol

294

202

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The underlying physical properties of microfluidic tools have led to new biological insights through the development of microsystems that can manipulate, mimic and measure biology at a resolution that has not been possible with macroscale tools. Microsystems readily handle sub-microlitre volumes, precisely route predictable laminar fluid flows and match both perturbations and measurements to the length scales and timescales of biological systems. The advent of fabrication techniques that do not require highly specialized engineering facilities is fuelling the broad dissemination of microfluidic systems and their adaptation to specific biological questions. We describe how our understanding of molecular and cell biology is being and will continue to be advanced by precision microfluidic approaches and posit that microfluidic tools — in conjunction with advanced imaging, bioinformatics and molecular biology approaches — will transform biology into a precision science.

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Section: Live-cell Imagingmentioning

confidence: 99%

Microfluidics: reframing biological enquiry

Duncombe

Tentori

Herr

2015

Nat Rev Mol Cell Biol

294

202

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“…Microfluidic platforms have gained interest for their high-throughput tissue analysis applications as they minimize cross talk [41], [42], permit manipulation of small fluidic volumes, and reduce reagent costs and tissue sample used, which are all desirable capabilities in biological multiplexing [43], [44]. A majority of the microfluidic devices used for IHC studies consist of parallel arrays of open channels that are reversibly bound atop a tissue section of interest.…”

Section: Spatially-patterned Multiplexing For Multiple Tissue Regionsmentioning

confidence: 99%

Recent developments in multiplexing techniques for immunohistochemistry

Dixon

Bathany

Tsuei

et al. 2015

Expert Review of Molecular Diagnostics

103

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Methods to detect immuno-labelled molecules at increasingly higher resolution, even when present at low levels, are revolutionizing immunohistochemistry (IHC). These technologies can be valuable for management and examination of rare patient tissue specimens, and for improved accuracy of early disease detection. The purpose of this mini-review is to highlight recent multiplexing methods that are candidates for more prevalent use in clinical research and potential translation to the clinic. Multiplex IHC methods, which permit identification of at least 3 and up to 30 discrete antigens, have been divided into whole section staining and spatially-patterned staining categories. Associated signal enhancement technologies that can enhance performance and throughput of multiplex IHC assays are also discussed. Each multiplex IHC technique, detailed herein, is associated with several advantages as well as tradeoffs that must be taken into consideration for proper evaluation and use of the methods.

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“…neurotransmission, 9,10 and have shown good sensitivities and detection capabilities for bio-applications. 11,12 Furthermore, recent developments in the fields of tissue-, cells-or organ-ana-chip [13][14][15][16] could take advantage of the versatility and ease of use of electrochemical sensors. Indeed, the biological use, in situ, of electrochemical techniques is now well-known, and integrating these systems into these new devices is now an obvious step to achieve a reliable, cheap and user-friendly biochip with integrated detection.…”

Section: Introductionmentioning

confidence: 99%

Delayed voltammetric with respect to amperometric electrochemical detection of concentration changes in microchannels

Trouillon

Gijs

2014

Lab Chip

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ARTICLEThis journal is © The Royal Society of Chemistry 2013 J. Name., 2013, 00, 1-3 | 1 The time response of an electrode incorporated into a fluidic channel to variations in analyte concentration of the outer-sphere redox probe ferrocenemethanol was investigated, both for amperometry (AMP) and cyclic voltammetry (CV). The experimental data show that the temporal resolution of CV is not as good as for AMP, as CV cannot properly detect fast concentration transients. The delayed response of CV was previously reported, for neurotransmitters, and mostly attributed to adsorption of the analyte to the electrode surface. By using an outer-sphere redox couple, we show that mass transport also significantly delays the response of CV. The experimental delay time in CV was understood from mass transfer limitations due to the relaxation of the diffusion layer during repeated potential scanning. Furthermore, a robust protocol for the analysis of fast concentration transients was established, using the impulse and modulation transfer functions of the system. This method was found to be more precise than the mere analysis of the undifferentiated traces in the time domain. As a proof of concept, the effect of increased viscosity was investigated, showing that AMP was more sensitive than CV to these variations. Overall, this analysis underlines further the enhanced temporal sensitivity of AMP over CV, at the expense of decreased chemical resolution, potentially having implications for in situ electrochemical detection of biologically relevant molecules.

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Exploring Living Multicellular Organisms, Organs, and Tissues Using Microfluidic Systems

Cited by 66 publications

References 216 publications

Microfluidics: reframing biological enquiry

Microfluidics: reframing biological enquiry

Recent developments in multiplexing techniques for immunohistochemistry

Delayed voltammetric with respect to amperometric electrochemical detection of concentration changes in microchannels

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