"Exploring the synergistic effect of Celery and Chinese kale on Antioxidant and Anti-Pancreatic Lipase and Anti-Acetylcholinesterase activities"

Abstract

It has been well known that vegetables provided numerous health benefits and mixed recipe derived local vegetables have been used in traditional folklore. Therefore, this study aimed to observe the synergistic effect of the mixed water extract of Celery and Chinese kale on chemical compositions using GC-MS analysis and the biological activity including antioxidant activity and inhibited acetylcholinesterase (AChE) and suppressed pancreatic lipase by in vitro study. The GC-MS analysis of the chemical compounds showed the presence of Pyrrolidine, N,N-Dimethylaminoethanol, 2,3-Butanediol, Dimethyl trisulfide, Pentane, 3-methyl-, 3H-1,2,4-Triazol-3-one, 1,2-dihydro-, p-Cresol, 3-Butylisobenzofuran-1(3H)-one, Senkyunolide, n-Hexadecanoic acid, and Phytol. The Mixed extract significantly increased in total phenolic compound but no different in flavonoid content when compared with the extract alone. The biological activity showed that the potentials of antioxidant activity, inhibited pancreatic lipase activity and Anti-AChE activity of the mixed extract significantly increased than extract alone. The combination between celery and chinese kale extract showed high potential in antioxidant activity, inhibition of pancreatic lipase and AChE activity due to its syngenetic effect. Therefore, the combined extract is suitable for developing the functional food with positive health effects that extend beyond their nutritional value such as nutraceuticals products with mitigating hyperlipidemia and improving cognitive function.

Selective green leafy vegetables and their synergistic combination approach as natural anti-diabetic agents: therapeutic potential

“…In combination-1, several compounds that appear in A. cepa were also seen in combination-1 and insigni cant compounds in A. cepa were not detected in combination-1, such as ribofuranose, isoleucine, phenylalanine, gluconic acid, and 2,3,4-trihydroxybutyric acid. In addition, several different compounds from A. cepa appeared in combination-1, which might have come from A. graveolens L., which has been previously reported in A. graveolens L., including 2,3-butanediol 34 , mannitol, alanine, asparagine 41 , galactitol 53 , and 9,12octadecadienoic acid 40 . A synergistic combination of the compounds promoted the higher bioactivity of the GLVs.…”

“…The subsequent increase in activity was seen for LPI, with a moderate increase in activity (p < 0.05); the highest activity was from combination-7 (43.06%). Similarly, Khongrum, et al 34 reported that the combination of celery and Chinese kale extract showed more signi cant LPI activity (IC 50 0.048 mg/mL).…”

“…The results demonstrated that all seven combinations had higher TPC content, almost comparable to the highest TPC of an individual sample, i.e., A. graveolens L. In addition, no signi cant difference was observed in the TPC of all seven combinations (p ≥ 0.05). Similarly, Khongrum, et al 34 reported that combining celery and Chinese kale synergized the TPC. However, there was a signi cant difference in other activities.…”

The Effect of Manalagi Apple Peel Addition on the Quality of Celery Herbal Tea

“…Based on Figure 3B it can be seen that the total phenol has decreased due to the longer drying time. [15] The longer drying process is being done, the phenolic compounds can be damaged. The drying process make the phenolic compounds oxidationed caused heat treatment which can damage the phenolic compounds.…”

“…In addition, the heating process during drying process also affects the total phenolic, because phenolic compounds are easily oxidized with over heat. So if the drying time is longer, the total phenolic will be decreased [15].…”