Oxidative stress and inflammation play key roles in the pathophysiology in the pathophysiology of dyslipidemia, which are positive risks that increase atherosclerosis leading to important healthcare problems. Therefore, we aimed to study the antioxidant, anti-inflammatory, and lipid-lowering effects of jelly drink containing polyphenol-rich roselle calyces extract and passion fruit juice with pulp concentrate (RP jelly drink) in comparison to a placebo jelly drink for 8 weeks. Forty-three adults with dyslipidemia were randomly assigned into two groups: the RP jelly drink group and the placebo group. Glucose, total cholesterol (TC) triglyceride (TG), low-density lipoprotein-cholesterol (LDL-C), high-density lipoprotein-cholesterol (HDL-C), oxidative stress biomarkers, inflammatory parameters, and monocyte chemotactic protein-1 (MCP-1) were measured with fasting blood samples at baseline, 4 weeks and 8 weeks of intervention. Results showed a significant decrease in LDL-C and TG, respectively, after 8 weeks of RP jelly drink consumption (LDL-C: 107.63 ± 22.98   m g / d L ; TG: 109.79 ± 38.83   m g / d L ) compared to baseline measurements (LDL-C: 128.43 ± 32.74   m g / d L ; TG: 132.33 ± 75.11   m g / d L ). These may be possible due to reduced inflammation and improvements in oxidative stress, as demonstrated by the reduction of tumor necrosis factor- (TNF-) α and malondialdehyde (MDA), and the enhancement of glutathione (GSH) after consuming the RP jelly drink for 8 weeks. However, no significant differences of treatment on glucose, total cholesterol, MCP-1, interleukin-6, and interleukin-10 were observed. In conclusion, daily consumption of RP jelly drink for 8 weeks resulted in significant improvement in lipid profiles in subjects with dyslipidemia. However, more research is needed to assess its nutritional and functional potential.

It has been well known that vegetables provided numerous health benefits and mixed recipe derived local vegetables have been used in traditional folklore. Therefore, this study aimed to observe the synergistic effect of the mixed water extract of Celery and Chinese kale on chemical compositions using GC-MS analysis and the biological activity including antioxidant activity and inhibited acetylcholinesterase (AChE) and suppressed pancreatic lipase by in vitro study. The GC-MS analysis of the chemical compounds showed the presence of Pyrrolidine, N,N-Dimethylaminoethanol, 2,3-Butanediol, Dimethyl trisulfide, Pentane, 3-methyl-, 3H-1,2,4-Triazol-3-one, 1,2-dihydro-, p-Cresol, 3-Butylisobenzofuran-1(3H)-one, Senkyunolide, n-Hexadecanoic acid, and Phytol. The Mixed extract significantly increased in total phenolic compound but no different in flavonoid content when compared with the extract alone. The biological activity showed that the potentials of antioxidant activity, inhibited pancreatic lipase activity and Anti-AChE activity of the mixed extract significantly increased than extract alone. The combination between celery and chinese kale extract showed high potential in antioxidant activity, inhibition of pancreatic lipase and AChE activity due to its syngenetic effect. Therefore, the combined extract is suitable for developing the functional food with positive health effects that extend beyond their nutritional value such as nutraceuticals products with mitigating hyperlipidemia and improving cognitive function.

Probiotics have the potential as a multi-target approach to modulate hypercholesterolemia associated with premature atherosclerosis. Various strains of Lactobacillus paracasei have been reported to affect hypercholesterolemia positively. This study aimed to investigate the effects of L. paracasei TISTR 2593 on lipid profile, cholesterol metabolism, and atherosclerosis according to the registration of Thai Clinical Trial Registry as identification number TCTR 20220917002. A total of 50 participants with hypercholesterolemia were randomly and equally assigned to consume L. paracasei TISTR 2593 or a placebo in maltodextrin capsules daily. Biomarkers of lipid profiles, oxidative stress state, inflammatory state, and other biological indicators were examined on days 0, 45, and 90. The results showed that subjects taking the L. paracasei TISTR 2593 could significantly reduce the level of serum low-density lipoprotein-cholesterol (p < 0.05), malondialdehyde (p < 0.001), and tumor necrosis factor-α (p < 0.01). Moreover, L. paracasei TISTR 2593 increased the level of serum apolipoprotein E (p < 0.01) and adiponectin (p < 0.001) significantly. No changes in serum total cholesterol, high-density lipoprotein-cholesterol, triglyceride, total bile acids, and monocyte chemoattractant protein-1 were observed during L. paracasei TISTR 2593 supplementation. Therefore, L. paracasei TISTR 2593 could be an adjuvant probiotic supplement to ameliorate hypercholesterolemia and prevent or delay the development of atherosclerosis.