Exposure of the Pig CNS for Histological Analysis: A Manual for Decapitation, Skull Opening, and Brain Removal

Bjarkam, Carsten Reidies; Orłowski, Dariusz; Tvilling, Laura; Bech, Johannes; Glud, Andreas Nørgaard; Sørensen, Jørn Nygaard

doi:10.3791/55511-v

Cited by 2 publications

(1 citation statement)

References 0 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Traditional animal models used in the experiment, such as fruit flies, zebrafish, and rodents, such as rats and mice, are often used to study the relevant single effects of a gene on biological pathways in human diseases, but often fail to represent the complexity of human physiology; gorillas, monkeys, and apes cannot be used in large numbers due to strict ethical issues 17,18 . In recent years, miniature pigs, a new type of laboratory animal with the advantages of high similarity to humans and few ethical issues, have been widely used in medical teaching, cardiovascular research, drug evaluation, and disease infection model 19,20 .…”

Section: Discussionmentioning

confidence: 99%

Brain structure analysis of different age groups of Diannan small‐ear pigs

Liu

Fang

et al. 2022

Ibrain

View full text Add to dashboard Cite

The objective of the study is to investigate the brain development and atrophy of Diannan small-ear pigs in different ages using magnetic resonance imaging (MRI). A total of 12 Diannan small-ear pigs were included and divided into the young group, adult group, and middle-and-old age (M&O) group according to their age. The brain structure of pigs was scanned using MRI, and the brain data obtained were statistically analyzed by signal conversion and image reconstruction. Compared with the young group, the signals of most brain structures in the adult group and M&O group were significantly decreased (p < 0.05). Compared with the adult group, the signal intensity of the right caudate nucleus and the right lateral ventricle in the M&O group was significantly increased, while the signal intensity of other regions was almost significantly decreased (p < 0.05). Compared with the young group, both adult and M&O groups had some degree of brain atrophy. Brain atrophy in the precuneus and the inferior temporal gyrus was more predominant in the M&O group in comparison with the adult group. The present study demonstrated that the brain signal of Diannan small-ear pigs gradually diminished with age, while the degree of brain atrophy was the opposite, providing the basic data on the brain of Diannan small-ear pigs.

show abstract

Section: Discussionmentioning

confidence: 99%