Expression and active testing of VP7 from GCRV (Grass carp reovirus) fused with cholera toxin B subunit in rice calli

Zhang, Qiusheng; Xu, Binglian; Pan, Jiajia; Liu, Danyang; Lv, Ruoxian; Yan, Dawei

doi:10.1016/j.pep.2019.02.007

Cited by 10 publications

(4 citation statements)

References 37 publications

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“…In addition to determining the localization of the recombinant antigen, its uptake can also be influenced by the incorporation of sequences encoding molecular adjuvants (CTBs, LTBs, flagellin, CPPs) and the creation of unique fusion proteins. 84,185,186 This procedure is not necessarily limited to a production of a single antigen, but several studies have focused on the preparation of so-called multivalent vaccines combining antigens of various pathogens or many epitopes of one pathogen. 49,[187][188][189] Importantly, plant production systems generate active recombinant antigens with adequate post-translational modifications.…”

Section: Benefits Of Plant-based Oral Vaccinesmentioning

confidence: 99%

“…Two capsid proteins MCP and VP7, from fish iridovirus and reovirus, respectively, were successfully expressed in rice calli (Oryza sativa). 186,223 Even though the plant-made recombinant VP7 protein fused with CTB was biologically active and bound to GM1-ganglioside, its immunostimulatory activity has not been tested. On the other hand, recombinant MCP showed a protective effect on fish orally immunized with transgenic rice calli with a significant increase in antibody levels, preventing the development of iridovirus infection symptoms.…”

Section: First Steps Of Fish Vaccines Produced In Plantsmentioning

confidence: 99%

“…Such recombinant protein stored in rice seeds remained stable for more than one and a half years, retaining its antigenic activity. In addition to determining the localization of the recombinant antigen, its uptake can also be influenced by the incorporation of sequences encoding molecular adjuvants (CTBs, LTBs, flagellin, CPPs) and the creation of unique fusion proteins 84,185,186 . This procedure is not necessarily limited to a production of a single antigen, but several studies have focused on the preparation of so‐called multivalent vaccines combining antigens of various pathogens or many epitopes of one pathogen 49,187–189 …”

Section: Molecular Farming Of Fish Vaccinesmentioning

confidence: 99%

“…Rice cell suspension culture is another system used for antigen production. Two capsid proteins MCP and VP7, from fish iridovirus and reovirus, respectively, were successfully expressed in rice calli ( Oryza sativa ) 186,223 . Even though the plant‐made recombinant VP7 protein fused with CTB was biologically active and bound to GM1‐ganglioside, its immunostimulatory activity has not been tested.…”

Section: Molecular Farming Of Fish Vaccinesmentioning

confidence: 99%

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Molecular farming: Expanding the field of edible vaccines for sustainable fish aquaculture

Mičúchová

Piačková

Frébort

et al. 2022

Reviews in Aquaculture

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Vaccines represent one of the most promising strategies for mitigating economic losses imposed by infectious diseases to global aquaculture. While the majority of commercial vaccines employ injection as the main route of delivery, a substantial body of research explores other avenues for the stimulation of protective immunity. Oral vaccines, representing the most favourable and convenient solution for the aquaculture industry, have been at the forefront of scientific interest for the last couple of decades. The orchestrated effort resulted in identifying the main roadblocks on the path to an ideal edible vaccine and provided several ingenious solutions improving the activation of immunity, ensuring the stability of administered antigens and their uptake upon the passage through the stomach. In the presented review, we describe advances in the available knowledge of the processes prerequisite for developing protective mucosal vaccines and focus readers' attention on the untapped potential of plant‐based production systems in this effort. We propose that these approaches not only meet production demands but also fulfil all requirements of an oral vaccine, addressing all the obstacles, previously solved via separate strategies, in one platform. Thus, combined with the available knowledge of molecular adjuvants and produced for a fraction of the cost, plant‐based production, so‐called molecular farming, is an ideal candidate for vaccine development, paving the road to sustainable aquaculture.

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