Expression and Localization of α-amylase in the Submandibular and Sublingual Glands of Mice

Yamagishi, Ryoko; Wakayama, Tomohiko; Nakata, Hiroki; Adthapanyawanich, Kannika; Kumchantuek, Tewarat; Yamamoto, Miyuki; Iseki, Shoichi

doi:10.1267/ahc.14005

Cited by 12 publications

(13 citation statements)

References 21 publications

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“…It is the first enzyme that begins the process of digestion by breaking complex carbohydrate. Interestingly, it was believed that AMY1 was produced by ductal cells in mouse submandibular glands until the recent study detecting its strong expression in the acinar cells of parotid and weaker expression in submandibular glands of mice [37]. In our present study, we found for the first time that MIST1 alone can upregulate AMY1 expression in mMSCs.…”

Section: Discussionsupporting

confidence: 58%

MIST1, an Inductive Signal for Salivary Amylase in Mesenchymal Stem Cells

Mona

Miller

et al. 2019

IJMS

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Sjögren’s syndrome (SjS) is an autoimmune disease that destroys the salivary glands and results in severe dry mouth. Mesenchymal stem cell (MSC) transplantation has been recently proposed as a promising therapy for restoring cells in multiple degenerative diseases. We have recently utilized advanced proteomics biochemical assays to identify the key molecules involved in the mesenchymal-epithelial transition (MET) of co-cultured mouse bone-marrow-derived MSCs mMSCs with primary salivary gland cells. Among the multiple transcription factors (TFs) that were differentially expressed, two major TFs were selected: muscle, intestine, and stomach expression-1 (MIST1) and transcription factor E2a (TCF3). These factors were assessed in the current study for their ability to drive the expression of acinar cell marker, alpha-salivary amylase 1 (AMY1), and ductal cell marker, cytokeratin19 (CK19), in vitro. Overexpression of MIST1-induced AMY1 expression while it had little effect on CK19 expression. In contrast, TCF3 induced neither of those cellular markers. Furthermore, we have identified that mMSCs express muscarinic-type 3 receptor (M3R) mainly in the cytoplasm and aquaporin 5 (AQP5) in the nucleus. While MIST1 did not alter M3R levels in mMSCs, a TCF3 overexpression downregulated M3R expressions in mMSCs. The mechanisms for such differential regulation of glandular markers by these TFs warrant further investigation.

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Section: Discussionsupporting

confidence: 58%

MIST1, an Inductive Signal for Salivary Amylase in Mesenchymal Stem Cells

Mona

Miller

et al. 2019

IJMS

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“…30 It was observed that the parotid gland in the experimental group showed multifocal clusters of mononuclear inflammatory cells, but with maintenance of normal histological structure and the submandibular and sublingual glands showed no significant change 50 days after infection.…”

Section: Discussionmentioning

confidence: 82%

Does Leishmaniasis disease alter the parenchyma and protein expression in salivary glands?

Júnior

Carvalho

Dantas

et al. 2015

Exp Biol Med (Maywood)

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Leishmaniasis is considered a serious public health problem in several regions in Brazil and worldwide. This research aimed to perform a histopathological and proteomic study of parotid, submandibular and sublingual glands of BALB/c mice infected by Leishmania (L) infantum chagasi using histological, immunohistochemical and epifluorescence techniques. Twelve isogenic BALB/ c male mice, around six-to eight-weeks old, were separated into two groups: the animals of the control group were injected with 0.15 ml of NaCl, while those in the experimental group were inoculated with 5 Â 10 6 amastigote forms of Leishmania (L) infantum chagasi by the ip route. After 50 days, animals were euthanized and major salivary glands were collected to perform histological, immunohistochemical and epifluorescence techniques using anti-Caspase-2, anti-Ki-67 and anti-b-catenin antibodies, respectively. The histological and morphometric evaluation showed clusters of mononuclear inflammatory cells and a higher area and perimeter of the parotid gland. However, none of the salivary glands had morphophysiological impairment. There was no immunoreactivity to the anti-caspase-2 antibody and Ki67 expression in acinar and ductal cells in both groups. According to the immunofluorescence staining, the b-catenin antibodies did not show nuclear expression, suggesting no uncontrolled proliferation. The data obtained in this study showed population and morphological stability of major salivary glands after 50 days post-infection by Leishmania (L) infantum chagasi.

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“…PTA preferentially stains proteins as shown by experiments with protein crystals (Maruyama et al, ) and cultured cells (Nishiyama et al, ). Therefore, the cell is considered to be a serous Demilune (Yamagishi et al, ), since these cells are known to have characteristic protein‐rich secretory granules.…”

Section: Resultsmentioning

confidence: 99%

Secretory glands and microvascular systems imaged in aqueous solution by atmospheric scanning electron microscopy (ASEM)

Yamazawa

Nakamura

Sato

et al. 2016

Microscopy Res & Technique

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Exocrine glands, e.g., salivary and pancreatic glands, play an important role in digestive enzyme secretion, while endocrine glands, e.g., pancreatic islets, secrete hormones that regulate blood glucose levels. The dysfunction of these secretory organs immediately leads to various diseases, such as diabetes or Sjögren's syndrome, by poorly understood mechanisms. Gland-related diseases have been studied by optical microscopy (OM), and at higher resolution by transmission electron microscopy (TEM) of Epon embedded samples, which necessitates hydrophobic sample pretreatment. Here, we report the direct observation of tissue in aqueous solution by atmospheric scanning electron microscopy (ASEM). Salivary glands, lacrimal glands, and pancreas were fixed, sectioned into slabs, stained with phosphotungstic acid (PTA), and inspected in radical scavenger d-glucose solution from below by an inverted scanning electron microscopy (SEM), guided by optical microscopy from above to target the tissue substructures. A 2- to 3-µm specimen thickness was visualized by the SEM. In secretory cells, cytoplasmic vesicles and other organelles were clearly imaged at high resolution, and the former could be classified according to the degree of PTA staining. In islets of Langerhans, the microvascular system used as an outlet by the secretory cells was also clearly observed. Microvascular system is also critically involved in the onset of diabetic complications and was clearly visible in subcutaneous tissue imaged by ASEM. The results suggest the use of in-solution ASEM for histology and to study vesicle secretion systems. Further, the high-throughput of ASEM makes it a potential tool for the diagnosis of exocrine and endocrine-related diseases.

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Expression and Localization of α-amylase in the Submandibular and Sublingual Glands of Mice

Cited by 12 publications

References 21 publications

MIST1, an Inductive Signal for Salivary Amylase in Mesenchymal Stem Cells

MIST1, an Inductive Signal for Salivary Amylase in Mesenchymal Stem Cells

Does Leishmaniasis disease alter the parenchyma and protein expression in salivary glands?

Secretory glands and microvascular systems imaged in aqueous solution by atmospheric scanning electron microscopy (ASEM)

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