Expression and regulation of transient receptor potential cation channel, subfamily M, member 2 (TRPM2) in human endometrium

Hiroi, Hisahiko; Momoeda, Mikio; Watanabe, Tôru; Ito, Masanori; Ikeda, Kazuhiro; Tsutsumi, Ryo; Hosokawa, Yumi; Koizumi, Minako; Zenri, Fumiko; Muramatsu, Masami; Taketani, Yuji; Inoue, Satoshi

doi:10.1016/j.mce.2012.10.015

Cited by 16 publications

(12 citation statements)

References 45 publications

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“…It is well known that estrogen and progesterone are the key factors in regulating endometrial stromal cells decidualization, and both estrogen and progesterone treatment of endometrial stromal cells accelerate TRPM2 mRNA expression. Taken together, these findings suggest that TRPM2 may play a role in endometrial decidualization (47).…”

Section: Calcium Calcium Channels Calcium Transporter and Binding Pmentioning

confidence: 58%

Ion/Water Channels for Embryo Implantation Barrier

et al. 2014

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Successful implantation involves three distinct processes, namely the embryo apposition, attachment, and penetration through the luminal epithelium of the endometrium to establish a vascular link to the mother. After penetration, stromal cells underlying the epithelium differentiate and surround the embryo to form the embryo implantation barrier, which blocks the passage of harmful substances to the embryo. Many ion/water channel proteins were found to be involved in the process of embryo implantation. First, ion/water channel proteins play their classical role in establishing a resting membrane potential, shaping action potentials and other electrical signals by gating the flow of ions across the cell membrane. Second, most of ion/water channel proteins are regulated by steroid hormone (estrogen or progesterone), which may have important implications to the embryo implantation. Last but not least, these proteins do not limit themselves as pure channels but also function as an initiator of a series of consequences once activated by their ligand/stimulator. Herein, we discuss these new insights in recent years about the contribution of ion/water channels to the embryo implantation barrier construction during early pregnancy.

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Section: Calcium Calcium Channels Calcium Transporter and Binding Pmentioning

confidence: 58%

Ion/Water Channels for Embryo Implantation Barrier

et al. 2014

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“…As a result, the expression at mRNA and protein levels may not always be synchronized (30,31). In their study, Hiroi et al (29) showed that the TRPM2 gene was an estrogen-dependent gene in cultured endometrial stromal cells. They also showed that there was no increase in TRPM2 mRNA expression in the medium and late endometrial proliferative phase where estrogen was the highest.…”

Section: Discussionmentioning

confidence: 99%

Is there any difference between endometrial hyperplasia and endometrial carcinoma in terms of expression of TRPM2 and TRPM7 ion channels?

Yalçın¹

2019

Turk J Med Sci

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Background/aim: This study compared TRPM2 and TRPM7 ion channel gene expression and immunohistochemical staining in endometrial hyperplasia and endometrium adenocarcinoma. Materials and methods: Sections were taken from paraffin blocks of 120 patients who were divided into 6 groups as follows: G1 (n = 20), proliferative endometrium (PE); G2 (n = 20), EH without atypia; G3 (n = 20), EH with atypia; G4 (n = 20), stage 1A, grade 1 EC; G5 (n = 20), stage 1A, grade 2 EC; and G6 (n = 20), stage 1A, grade 3 EC. TRPM2 and TRPM7 genes were analyzed with qRT-PCR in paraffin-embedded tissue samples. Under light microscopy, TRPM2 and TRPM7 immunostaining scores of the samples taken from polylysine slides were evaluated. Results: Compared to G1, TRPM2 mRNA gene expression was significantly downregulated in G3 and G5. TRPM2 immunoreactivity scores were similar in all groups. TRPM7 mRNA gene expression was significantly downregulated in G2, G3, and G6 when compared to G1. TRPM7 immunoreactivity scores were similar in G1, G2, and G3, but significantly decreased in G4, G5, and G6. Conclusion: Reduction in TRPM7 ion channel activity may be a progression marker for endometrial hyperplasia regardless of the atypical criteria.

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“…For example, TRPV6 expression can be positively regulated by estrogen during the follicular phase, both in endometrial biopsies and Ishikawa cells [ 41 ]. TRPM2 mRNA expression has been investigated in human endometrium and hESC, revealing an increase in TRPM2 expression upon estrogen treatment [ 42 ]. The co-application of estrogen and progesterone results in an increase of TRPC1 mRNA, whereas TRPC6 expression increases solely by application of estrogen on hESC [ 43 ].…”

Section: Discussionmentioning

confidence: 99%

“…TRPV1, TRPV2, TRPV4, TRPM4, TRPM7, TRPC1, TRPC3, TRPC4, and TRPC6 mRNA was observed to be present in hESC of both endometriosis patients and controls. No expression of TRPM2 and TRPM3 was observed in both groups, although the former has been described to be expressed in hESC [ 42 ]. The ablation of TRPM2 and TRPM3 expression, however, can be explained by the loss of nerve endings when setting up the primary culture of hESC.…”

Section: Discussionmentioning

confidence: 99%

Functional Expression of TRP Ion Channels in Endometrial Stromal Cells of Endometriosis Patients

Persoons

Hennes

Clercq

et al. 2018

IJMS

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Endometriosis is a common gynecological disease that is characterized by the presence of functional endometrial-like lesions in the abdominal cavity. Aside from epithelial cells, these lesions consist of stromal cells that have the capacity to migrate, adhere, proliferate, and induce neuro- and lymphangiogenesis, which allows them to survive at ectopic locations. However, the exact underlying mechanisms that regulate these changes are yet to be elucidated. The common ground of these processes, however, is the second messenger, calcium. In this regard, members of the superfamily of transient receptor potential (TRP) ion channels, which are known to be calcium-permeable and expressed in the endometrium, have emerged as key regulators. Here, we assessed the molecular and functional expression of TRP channels in stromal cells isolated from the eutopic endometrium of endometriosis patients and controls. Using RT-qPCR, high mRNA levels of TRPV2, TRPV4, TRPM4, TRPM7, TRPC1, TRPC3, TRPC4, and TRPC6 were observed in the whole endometrium throughout the menstrual cycle. Additionally, and in line with previous reports of control patients, TRPV2, TRPV4, TRPC1/4, and TRPC6 were present in human endometrial stromal cells (hESC) from endometriosis patients both at the molecular and functional level. Moreover, proliferation and migration assays illustrated that these parameters were not affected in stromal cells from endometriosis patients. Furthermore, comparison between eutopic and ectopic endometrial samples revealed that the RNA expression pattern of TRP channels did not differ significantly. Collectively, although a functional expression of specific ion channels in hESCs was found, their expression did not correlate with endometriosis.

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Expression and regulation of transient receptor potential cation channel, subfamily M, member 2 (TRPM2) in human endometrium

Cited by 16 publications

References 45 publications

Ion/Water Channels for Embryo Implantation Barrier

Ion/Water Channels for Embryo Implantation Barrier

Is there any difference between endometrial hyperplasia and endometrial carcinoma in terms of expression of TRPM2 and TRPM7 ion channels?

Functional Expression of TRP Ion Channels in Endometrial Stromal Cells of Endometriosis Patients

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