Expression and self-assembly of cowpea chlorotic mottle virus-like particles in Pseudomonas fluorescens

Phelps, Jamie; Dao, Philip; Jin, H; Rasochova, Lada

doi:10.1016/j.jbiotec.2006.10.005

Cited by 31 publications

(25 citation statements)

References 30 publications

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“…26 Although other prokaryotic systems can also be considered, such as Pseudomonas fluorescens , 27 the wealth of knowledge surrounding E. coli production makes it popular, and therefore it has also been widely applied for the production of VLPs. Viral coat proteins can be expressed and spontaneously self-assembled in the bacterial cells, and this has been demonstrated for bacteriophages, such as Qβ 28 and MS2, 29 as well as for heterologous expression of other viruses, such as plant virus-based TMV 30 and mammalian virus-based hepatitis B virus (HBV) core particles.…”

Section: Viruses In a Materials Worldmentioning

confidence: 99%

Design of virus-based nanomaterials for medicine, biotechnology, and energy

2016

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Virus-based nanomaterials are versatile materials that naturally self-assemble and have relevance for a broad range of applications including medicine, biotechnology, and energy. This review provides an overview of recent developments in “chemical virology.” Viruses, as materials, provide unique nanoscale scaffolds that have relevance in chemical biology and nanotechnology, with diverse areas of applications. Some fundamental advantages of viruses, compared to synthetically programmed materials, include the highly precise spatial arrangement of their subunits into a diverse array of shapes and sizes and many available avenues for easy and reproducible modification. Here, we will first survey the broad distribution of viruses and various methods for producing virus-based nanoparticles, as well as engineering principles used to impart new functionalities. We will then examine the broad range of applications and implications of virus-based materials, focusing on the medical, biotechnology, and energy sectors. We anticipate that this field will continue to evolve and grow, with exciting new possibilities stemming from advancements in the rational design of virus-based nanomaterials.

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Section: Viruses In a Materials Worldmentioning

confidence: 99%

Design of virus-based nanomaterials for medicine, biotechnology, and energy

2016

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“…A Pseudomonas fluorescens (P. fluorescens) expression system is an efficient choice against E. coli , because of simple manipulation, high yields of active and soluble proteins, and large‐scale cultivation. Some differences between P. fluorescens and E. coli including the various sizes of genome, and diverse metabolic approaches can influence the generation of recombinant proteins . The capsid protein of a plant bromovirus , the cowpea chlorotic mottle virus (CCMV), has been recently expressed as a soluble form in P. fluorescens , and assembled into VLPs in vivo .…”

Section: Different Expression Methods For Generation Of Vlpmentioning

confidence: 99%

“…The capsid protein of a plant bromovirus , the cowpea chlorotic mottle virus (CCMV), has been recently expressed as a soluble form in P. fluorescens , and assembled into VLPs in vivo . This construct was structurally similar to the natural viral particles provided from plants …”

Section: Different Expression Methods For Generation Of Vlpmentioning

confidence: 99%

Different applications of virus‐like particles in biology and medicine: Vaccination and delivery systems

2015

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Virus-like particles (VLPs) mimic the whole construct of virus particles devoid of viral genome as used in subunit vaccine design. VLPs can elicit efficient protective immunity as direct immunogens compared to soluble antigens co-administered with adjuvants in several booster injections. Up to now, several prokaryotic and eukaryotic systems such as insect, yeast, plant, and E. coli were used to express recombinant proteins, especially for VLP production. Recent studies are also generating VLPs in plants using different transient expression vectors for edible vaccines. VLPs and viral particles have been applied for different functions such as gene therapy, vaccination, nanotechnology, and diagnostics. Herein, we describe VLP production in different systems as well as its applications in biology and medicine.

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“…For example, virus assembly [1,[6][7][8], stability and disassembly [9][10][11][12] and also nanomaterials [13][14][15][16][17].…”

Section: Introductionmentioning

confidence: 99%

Molecular characterization of the complete genomes of two new field isolates of Cowpea chlorotic mottle virus, and their phylogenetic analysis

Ali

Olsen

2011

Virus Genes

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Cowpea chlorotic mottle virus (CCMV, family Bromoviridae) is found worldwide and has been used as a model virus for a long time, but no data is available about the genetic diversity of field isolates. Recently, two new field isolates (Car1 and Car2) of CCMV obtained from cowpea showed distinct phenotypic symptoms when inoculated to cowpea. CCMV-Car1 induced severe mosaic and interveinal chlorosis, while CCMV-Car2 produced mild mottling and leaf rolling. Both isolates produced asymptomatic infection in Nicotiana benthamiana. The complete genome of both isolates was amplified by reverse transcription-polymerase chain reaction using specific primers against the CCMV sequences available in the GenBank database, cloned and sequenced. Both nucleotide and amino acid sequences were compared between the newly sequenced CCMV isolates and the three previously characterized CCMV strains (T, M1, and R). Phylogenetic analysis of the RNA 1 sequence showed that CCMV-Car1 was in a separate branch from the rest of the CCMV isolates while CCMV-Car2 grouped together with CCMV-R. On the basis of RNA 2 and RNA 3 sequences, two major groupings were obtained. One group included CCMV-Car1 and CCMV-Car2 isolates while the other contained CCMV-T, CCMV-M1, and CCMV-R strains. Recombination programs detected a potential recombination event in the RNA 1 sequence of CCMV-Car2 isolate but not in RNA 2 and RNA 3 sequences. The results showed that both mutations and recombination have played an important role in the genetic diversity of these two new isolates of CCMV.

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Expression and self-assembly of cowpea chlorotic mottle virus-like particles in Pseudomonas fluorescens

Cited by 31 publications

References 30 publications

Design of virus-based nanomaterials for medicine, biotechnology, and energy

Design of virus-based nanomaterials for medicine, biotechnology, and energy

Different applications of virus‐like particles in biology and medicine: Vaccination and delivery systems

Molecular characterization of the complete genomes of two new field isolates of Cowpea chlorotic mottle virus, and their phylogenetic analysis

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