Expression and significance of tumor infiltrating dendritic cells in renal cell carcinoma

Abstract

Objective:To study the expression of dendritic cells in human renal cell carcinoma and explore the cause, so to reveal the mechanism of escaping immune surveillance in RCC. Methods: The expressions of CD83+DCs, CDla+DCs,VEGF and TGF-I31 in tumoral, peritumoral and normal kidney tissues of RCC in 30 cases were detected by immunohistochemistry using streptavidin/peroxidese(SP) Results: CD83+DCs were mainly located in the peritumoral areas; whereas CDla+DCs, were mainly retained within the cancer nests. The number of CD83+DCs was inversely correlated with the clinical stage (P<0.05); but there were no significant correlations between the number of CD 1 a+DCs, and the clinical stage ( P>0.05 ). The expressions of CD83+DCs and CDla+DCs have significant difference between the tumoral, peritumoral and normal kidney tissues(P<0.001). The expression of VEGF and TGF-131 were significantly lower in samples with highly infiltrating CD83+DCs(P<0.05); Whereas CDIa+DCs were not (P>0.05). Conclusion: DC has the tendency to gathering in tumor, but because of the immunosuppressive cytokins, for example VEGF and TGF-[31, inhibits the maturation of DC, there are less mature TIDCs(CD83+TIDCs) in the tumoral tissues, they are mainly located in the peritumoral areas. This may contribute to the mechanism of escaping immune surveillance in RCC.