Expression levels of survivin, Bcl-2, and KAI1 proteins in cervical cancer and their correlation with metastasis

Abstract

ABSTRACT. Cervical cancer is associated with abnormal expression of multiple genes. Survivin and Bcl-2 proteins are apoptosis inhibitors. The tumor suppressor gene CD82, which encodes the protein KAI1, is downregulated in cervical cancer, and is associated with differentiation degree. We investigated the expression levels of three proteins and their correlation with metastasis in cervical cancer by comparing them in different cervical lesions. Immunohistochemistry was used to detect their three protein expression levels in the normal cervix, chronic cervicitis, cervical intraepithelial neoplasia (CIN) lesions, and cervical cancer. The relationships between the protein expression levels and tumor type, clinical stage, tissue differentiation, invasion, and metastasis were analyzed. Survivin and Bcl-2 expression levels in cervical cancer were significantly higher than in the normal cervix, chronic cervicitis, or CIN (P < 0.05). KAI1 expression was markedly lower in cervical cancer than in the normal cervix, chronic cervicitis, or CIN (P < 0.05). There was no statistical difference between the expression levels of the three proteins in CIN and chronic cervicitis, but there were differences in expression between CIN and normal cervical tissues (P < 0.05). Bcl-2 and survivin levels were positively correlated while KAI1 expression was negatively correlated with clinical stage. Survivin and KAI1 expression levels were associated with lymph node metastasis (P < 0.05), and KAI1 expression was positively related with differentiation degree (P < 0.05). Survivin, Bcl-2, and KAI1 are metastasis-related factors in cervical cancer. Overexpression of survivin and Bcl-2, and low expression of KAI1 promotes cervical cancer progress and metastasis.

miR-187 induces apoptosis of SiHa cervical carcinoma cells by downregulating Bcl-2

“…However, none of these have addressed the relationship between Bcl-2 and cervical cancer. Our study is the first to demonstrate that Bcl-2 also inhibits apoptosis in cervical cancer, identifying it as a potential target for molecular therapy based on regulation by miR-187 (Zhou and Wang, 2015). However, we cannot exclude other BCL-2 independent effects during miR-187-mediated cell apoptosis and requires further investigation.…”

The roles of CD82 expression in gastric cancer: a meta and bioinformatics analysis

“…A progressive down-regulation of CD82 was during colorectal mucosa-adenoma-the primary adenocarcinoma to the liver metastasis [45]. Zhou et al [46] found that CD82 expression was markedly lower in cervical cancer than in the normal cervix, chronic cervicitis, or cervical intraepithelial neoplasia. Reportedly, the promoter CpGSite methylation and LOH of CD82 contributed to its epigenetic repression [47,48].…”

The Effects of Cytotoxic Dose of Diclofenac on CD82/KAI1 in Cervical Cancer Cells in Cell Culture

“…Overexpression of survivin and Bcl-2, and low expression of KAI1 promotes cervical cancer progress and metastasis. [14] The studies suggest that KAI1 may be used as a promising prognostic marker and a possible therapeutic target for human melanoma. [15] Decreased KAI1 expression has been observed recently in various human cancers, including pancreatic, lung, hepatic, colorectal, breast, ovarian, esophageal, and cervical cancers.…”