Expression of CD147 and monocarboxylate transporters MCT1, MCT2 and MCT4 in porcine small intestine and colon

Sepponen, Kirsi; Ruusunen, Marita; Pakkanen, Jussi; Pösö, A.R.

doi:10.1016/j.tvjl.2006.05.015

Cited by 31 publications

(23 citation statements)

References 36 publications

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“…Additionally, the relative abundance of different monocarboxylate transporters in the intestinal tract of different pig breeds was studied by immunoblotting (Sepponen et al, 2007). A systematic comparison both at the mRNA level and protein level by immunohistochemistry has not yet been published.…”

Section: Discussionmentioning

confidence: 99%

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Expression of the monocarboxylate transporter 1 (MCT1) in cells of the porcine intestine

Welter

Claus

2008

Cell Biology International

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Uptake of energy into cells and its allocation to individual cellular compartments by transporters are essential for tissue homeostasis. The present study gives an analysis of MCT1 expression and its cellular occurrence in the porcine intestine. Tissue portions from duodenum, jejunum, ileum, colon ascendens, colon transversum and colon descendens were collected and prepared for immunohistochemistry, Western blot and real time RT-PCR. A 169bp porcine MCT1 cDNA fragment was amplified and published. MCT1 mRNA expression in the large intestine was 20 fold higher compared to the small intestine. Western blot detected a single protein band of 41kDa at a much higher amount of MCT1 protein in the large intestine vs. the small intestine. MCT1 protein was detected in mitochondrial fractions of the large but not the small intestine. Immunohistochemistry in the small intestine showed that immune cells in the lamina propria and in the lymphoid follicles primarily expressed MCT1 while in the colon epithelial cells were the main source of MCT1. In summary, cellular expression of MCT1 differs between epithelial cells in the colon and small intestine. A possible role of MCT1 for uptake of butyrate into immune cells and the overall role of MCT1 for intestinal immune cell function remains elusive.

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Section: Discussionmentioning

confidence: 99%

“…In rats (Orsenigo et al, 1999) and rabbits (Tamai et al, 1995) MCT1 could be identified in jejunal enterocytes. An actual study revealed both MCT1 and MCT4 in porcine small intestine by Western blot (Sepponen et al, 2007), but its allocation, e.g. by immunohistochemistry, to specific cell types was not investigated.…”

Section: Introductionmentioning

confidence: 99%

Expression of the monocarboxylate transporter 1 (MCT1) in cells of the porcine intestine

Welter

Claus

2008

Cell Biology International

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“…Gut site affected the expression of GLUT2, SMCT, GLP1 and GIP (P < 0.05) and of MCT1 (P = 0.05). 2 and 4 (Cuff and Shirazi-Beechey, 2004;Halestrap et al, 2004;Iwanaga et al, 2006;Sepponen et al, 2007). The intestinal SCFA transporter expression is substrate induced and should therefore respond to changes in luminal SCFA concentrations (Sepponen et al, 2007).…”

Section: Discussionmentioning

confidence: 99%

“…2 and 4 (Cuff and Shirazi-Beechey, 2004;Halestrap et al, 2004;Iwanaga et al, 2006;Sepponen et al, 2007). The intestinal SCFA transporter expression is substrate induced and should therefore respond to changes in luminal SCFA concentrations (Sepponen et al, 2007). Only the expression of SMCT was up-regulated in pigs fed the EMS diet which might have indicated a greater SCFA or lactate availability in the upper small intestine.…”

Section: Discussionmentioning

confidence: 99%

Enzymatically modified starch up-regulates expression of incretins and sodium-coupled monocarboxylate transporter in jejunum of growing pigs

Metzler‐Zebeli

Ertl

Grüll³

et al. 2017

Animal

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Dietary effects on the host are mediated via modulation of the intestinal mucosal responses. The present study investigated the effect of an enzymatically modified starch (EMS) product on the mucosal expression of genes related to starch digestion, sugar and short-chain fatty acid (SCFA) absorption and incretins in the jejunum and cecum in growing pigs. Moreover, the impact of the EMS on hepatic expression of genes related to glucose and lipid metabolism, and postprandial serum metabolites were assessed. Barrows (n = 12/diet; initial BW 29 kg) were individually fed three times daily with free access to a diet containing either EMS or waxy corn starch as control (CON) for 10 days. The enzymatic modification led to twice as many α-1,6-glycosidic bonds (~8%) in the amylopectin fraction in the EMS in comparison with the non-modified native waxy corn starch (4% α-1,6-glycosidic bonds). Linear discriminant analysis revealed distinct clustering of mucosal gene expression for EMS and CON diets in jejunum. Compared with the CON diet, the EMS intake up-regulated jejunal expression of sodium-coupled monocarboxylate transporter (SMCT), glucagon-like peptide-1 (GLP1) and gastric inhibitory polypeptide (GIP) (P < 0.05) and intestinal alkaline phosphatase (ALPI) (P = 0.08), which may be related to greater luminal SCFA availability, whereas cecal gene expression was unaffected by diet. Hepatic peroxisome proliferator-activated receptor γ (PPARγ) expression tended (P = 0.07) to be down-regulated in pigs fed the EMS diet compared with pigs fed the CON diet, which may explain the trend (P = 0.08) of 30% decrease in serum triglycerides in pigs fed the EMS diet. Furthermore, pigs fed the EMS diet had a 50% higher (P = 0.03) serum urea concentration than pigs fed the CON diet potentially indicating an increased use of glucogenic amino acids for energy acquisition in these pigs. Present findings suggested the jejunum as the target site to influence the intestinal epithelium and altered lipid and carbohydrate metabolism by EMS feeding.

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“…The presence of MCT1 has been detected by immuno-staining on the surface of adult mouse gastric surface epithelium (Nakai et al, 2006). The distribution of MCT1 was studied in the small intestine and colon of pigs (Ritzhaupt et al, 1998a and1998b;Sepponen et al, 2007), and its presence was enhanced by the luminal production of butyrate after bacteria fermentation. The addition of different OA to the diet can differently affect the morphology and function of the stomach of weaned pigs.…”

Section: Organic Acidsmentioning

confidence: 99%

Impact of bioactive substances on the gastrointestinal tract and performance of weaned piglets: a review

Lallès

Bosi

Janczyk

et al. 2009

Animal

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The EU ban on in-feed antibiotics has stimulated research on weaning diets as a way of reducing post-weaning gut disorders and growth check in pigs. Many bioactive components have been investigated but only few have shown to be effective. Amongst these, organic acids (OA) have been shown to exert a bactericidal action mediated by non-dissociated OA, by lowering gastric pH, increasing gut and pancreas enzyme secretion and improving gut wall morphology. It has been postulated that they may also enhance non-specific immune responses and improve disease resistance. In contrast, relatively little attention has been paid to the impact of OA on the stomach but recent data show they can differently affect gastric histology, acid secretion and gastric emptying. Butyrate and precursors of butyric acid have received special attention and although promising results have been obtained, their effects are dependent upon the dose, treatment duration, initial age of piglets, gastrointestinal site and other factors. The amino acids (AA) like glutamine, tryptophan and arginine are supportive in improving digestion, absorption and retention of nutrients by affecting tissue anabolism, stress and (or) immunity. Glutamine, cysteine and threonine are important for maintaining mucin and permeability of intestinal barrier function. Spray-dried plasma (SDP) positively affects gut morphology, inflammation and reduces acquired specific immune responses via specific and a-specific influences of immunoglobulins and other bioactive components. Effects are more pronounced in early-weaned piglets and under poorer health conditions. Little interaction between plasma protein and antibiotics has been found, suggesting distinct modes of action and additive effects. Bovine colostrum may act more or less similarly to SDP. The composition of essential oils is highly variable, depending on environmental and climatic conditions and distillation methods. These oils differ widely in their antimicrobial activity in vitro and some components of weaning diets may decrease their activity. Results in young pigs are highly variable depending upon the product and doses used. These studies suggest that relatively high concentrations of essential oils are needed for beneficial effects to be observed and it has been assumed that these plant extracts mimic most of the effects of antibiotics active on gut physiology, microbiology and immunology. Often, bioactive substances protective to the gut also stimulate feed intake and growth performance. New insights on the effects of selected OA and AA, protein sources (especially SDP, bovine colostrum) and plant extracts with anti-bacterial activities on the gut are reported in this review.

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Expression of CD147 and monocarboxylate transporters MCT1, MCT2 and MCT4 in porcine small intestine and colon

Cited by 31 publications

References 36 publications

Expression of the monocarboxylate transporter 1 (MCT1) in cells of the porcine intestine

Expression of the monocarboxylate transporter 1 (MCT1) in cells of the porcine intestine

Enzymatically modified starch up-regulates expression of incretins and sodium-coupled monocarboxylate transporter in jejunum of growing pigs

Impact of bioactive substances on the gastrointestinal tract and performance of weaned piglets: a review

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